JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on February 21, 2003
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
278/9/7500    most recent
M206780200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Borgatti, M.
Right arrow Articles by Gambari, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Borgatti, M.
Right arrow Articles by Gambari, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print November 20, 2002
J. Biol. Chem, 10.1074/jbc.M206780200
Submitted on July 8, 2002
Revised on November 19, 2002
Accepted on November 20, 2002

Transcription factor decoy molecules based on a PNA-DNA chimera mimicking Sp1 binding sites

Monica Borgatti, Ilaria Lampronti, Alessandra Romanelli, Carlo Pedone, Michele Saviano, Nicoletta Bianchi, Carlo Mischiati, and Roberto Gambari

Biochemistry and Molecular Biology, University of Ferrara, Ferrara 44100

Corresponding Author: gam{at}unife.it

Peptide nucleic acids (PNAs) are DNA mimicking molecules in which the sugar-phosphate backbone is replaced by a pseudopeptide backbone composed of N-(2-aminoethyl)glycine units. We determined whether double-stranded molecules based on PNAs and PNA-DNA-PNA (PDP) chimeras could be capable of stable interactions with nuclear proteins belonging to Sp1 transcription factor family and, therefore, could act as decoy reagents able to inhibit molecular interactions between Sp1 and DNA. Since the structure of PNA/PNA hybrids is very different from that of DNA/DNA double helix, they could theoretically alter the molecular structure of the double stranded PNA-DNA-PNA chimeras, perturbing interactions with specific transcription factors. We found that PNA-based hybrids do not inhibit Sp1/DNA interactions. By contrast, hybrid molecules based on PNA-DNA-PNA chimeras are very effective decoy molecules, encouraging further experiments focused on possible use of these molecules for the development of potential agents for a decoy approach in gene therapy. In this respect, the finding that PDP-based decoy molecules are more resistant than DNA/DNA hybrids to enzymatic degradation appears to be of great interest. Furthermore, their resistance can be even improved after complexation with cationic liposomes to which PDP/PDP chimeras are able to bind in virtue of their internal DNA structure.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Evid Based Complement Alternat MedHome page
I. Lampronti, M. T.H. Khan, M. Borgatti, N. Bianchi, and R. Gambari
Inhibitory Effects of Bangladeshi Medicinal Plant Extracts on Interactions between Transcription Factors and Target DNA Sequences
Evid. Based Complement. Altern. Med., September 1, 2008; 5(3): 303 - 312.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
V. Bezzerri, M. Borgatti, E. Nicolis, I. Lampronti, M. C. Dechecchi, I. Mancini, P. Rizzotti, R. Gambari, and G. Cabrini
Transcription Factor Oligodeoxynucleotides to NF-{kappa}B Inhibit Transcription of IL-8 in Bronchial Cells
Am. J. Respir. Cell Mol. Biol., July 1, 2008; 39(1): 86 - 96.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. C. Dechecchi, E. Nicolis, V. Bezzerri, A. Vella, M. Colombatti, B. M. Assael, Y. Mettey, M. Borgatti, I. Mancini, R. Gambari, et al.
MPB-07 Reduces the Inflammatory Response to Pseudomonas aeruginosa in Cystic Fibrosis Bronchial Cells
Am. J. Respir. Cell Mol. Biol., May 1, 2007; 36(5): 615 - 624.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
M. Abdelrahim, C. H. Baker, J. L. Abbruzzese, and S. Safe
Tolfenamic acid and pancreatic cancer growth, angiogenesis, and Sp protein degradation.
J Natl Cancer Inst, June 21, 2006; 98(12): 855 - 868.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. Feriotto, A. Finotti, P. Volpe, S. Treves, S. Ferrari, C. Angelelli, F. Zorzato, and R. Gambari
Myocyte Enhancer Factor 2 Activates Promoter Sequences of the Human A{beta}H-J-J Locus, Encoding Aspartyl-{beta}-Hydroxylase, Junctin, and Junctate
Mol. Cell. Biol., April 15, 2005; 25(8): 3261 - 3275.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. Crinelli, M. Bianchi, L. Gentilini, L. Palma, M. D. Sorensen, T. Bryld, R. B. Babu, K. Arar, J. Wengel, and M. Magnani
Transcription factor decoy oligonucleotides modified with locked nucleic acids: an in vitro study to reconcile biostability with binding affinity
Nucleic Acids Res., March 29, 2004; 32(6): 1874 - 1885.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.