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Papers In Press, published online ahead of print August 26, 2002
Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, Kentucky 40536
Corresponding Author: waechte{at}pop.uky.edu
The CWH8 gene in Saccharomyces cerevisiae has recently been shown to encode a dolichyl pyrophosphate (Dol-P-P) phosphatase associated with crude microsomal fractions (Fernandez et al. (2001) J. Biol. Chem. 276, 41455-41464). Mutations in CWH8 result in the accumulation of Dol-P-P, deficiency in lipid intermediate synthesis, defective protein N-glycosylation and a reduced growth rate. A cDNA (DOLPP1, accession number: AB030189) from mouse brain encoding a homologue of the yeast CWH8 gene, is now shown to complement the defects in growth and protein N-glycosylation, and to correct the accumulation of Dol-P-P in the cwh8D yeast mutant. Northern blot analyses demonstrate a wide distribution of the DOLPP1 mRNA in mouse tissues. Overexpression of Dolpp1p in yeast, COS and Sf9 cells produces substantial increases in Dol-P-P phosphatase activity, but not in dolichyl monophosphate (Dol-P) or phosphatidic acid (PA) phosphatase activities, in microsomal fractions. Subcellular fractionation and immunofluorescence studies localize the enzyme encoded by DOLPP1 to the endoplasmic reticulum (ER) of COS cells. The results of protease-sensitivity studies with microsomal vesicles from the lpp1delta/dpp1delta yeast mutant expressing DOLPP1 are consistent with Dolpp1p having a lumenally-oriented active site. The sequence of the DOLPP1 cDNA predicts a polypeptide with 238 amino acids, and a new polypeptide corresponding to 27 kDa is observed when DOLPP1 is expressed in yeast, COS and Sf9 cells. This study is the first identification and characterization of a cDNA clone encoding an essential component of a mammalian lipid pyrophosphate phosphatase that is highly specific for Dol-P-P and a resident of the ER. The specificity, subcellular location and topological orientation of the active site described in the current study strongly support a role for Dolpp1p in the recycling of Dol-P-P discharged during protein N-glycosylation reactions on the lumenal leaflet of the ER in mammalian cells.
J. Biol. Chem, 10.1074/jbc.M207076200
Submitted on July 15, 2002
Revised on August 26, 2002
Accepted on August 26, 2002
Identification and characterization of a cDNA encoding a dolichyl pyrophosphate phosphatase located in the ER of mammalian cells
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