| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print December 5, 2002
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110
Corresponding Author: sibley{at}borcim.wustl.edu
Host cell invasion by apicomplexan parasites is accompanied by the rapid, polarized secretion of parasite proteins that are involved in cell attachment. The Toxoplasma gondii micronemal protein MIC2 contains several extracellular adhesive domains, a transmembrane domain, and a short cytoplasmic tail. Following apical secretion, MIC2 is transiently present on the parasite surface, before being translocated backward and released by proteolytic cleavage. Mutations in the extracellular domain of MIC2, directly upstream of the transmembrane domain, prevented processing and release of the soluble protein into the supernatant. A conserved basic residue in MIC2 was essential for cleavage, and basic residues are similarly positioned in other micronemes proteins. Following induction of secretion, MIC2 processing mutants were stably expressed on the surface of the parasite. Surface MIC2-expressing mutants showed increased adhesion to host cells, yet were impaired in their capacity to invade. These data demonstrate that proteolysis is essential for releasing cell surface adhesins prior to cell entry by apicomplexan parasites.
J. Biol. Chem, 10.1074/jbc.M209837200
Submitted on September 25, 2002
Revised on December 3, 2002
Accepted on December 5, 2002
C-terminal processing of the Toxoplasma protein MIC2 is essential for invasion into host cells
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Brossier, G. L. Starnes, W. L. Beatty, and L. D. Sibley Microneme Rhomboid Protease TgROM1 Is Required for Efficient Intracellular Growth of Toxoplasma gondii Eukaryot. Cell, April 1, 2008; 7(4): 664 - 674. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kessler, A. Herm-Gotz, S. Hegge, M. Rauch, D. Soldati-Favre, F. Frischknecht, and M. Meissner Microneme protein 8 - a new essential invasion factor in Toxoplasma gondii J. Cell Sci., April 1, 2008; 121(7): 947 - 956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Brydges, X. W. Zhou, M.-H. Huynh, J. M. Harper, J. Mital, K. D. Z. Adjogble, W. Daubener, G. E. Ward, and V. B. Carruthers Targeted Deletion of MIC5 Enhances Trimming Proteolysis of Toxoplasma Invasion Proteins Eukaryot. Cell, December 1, 2006; 5(12): 2174 - 2183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. L. Starnes, T. J. Jewett, V. B. Carruthers, and L. D. Sibley Two Separate, Conserved Acidic Amino Acid Domains within the Toxoplasma gondii MIC2 Cytoplasmic Tail Are Required for Parasite Survival J. Biol. Chem., October 13, 2006; 281(41): 30745 - 30754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Harper, M.-H. Huynh, I. Coppens, F. Parussini, S. Moreno, and V. B. Carruthers A Cleavable Propeptide Influences Toxoplasma Infection by Facilitating the Trafficking and Secretion of the TgMIC2-M2AP Invasion Complex Mol. Biol. Cell, October 1, 2006; 17(10): 4551 - 4563. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. O'Donnell, F. Hackett, S. A. Howell, M. Treeck, N. Struck, Z. Krnajski, C. Withers-Martinez, T. W. Gilberger, and M. J. Blackman Intramembrane proteolysis mediates shedding of a key adhesin during erythrocyte invasion by the malaria parasite J. Cell Biol., September 25, 2006; 174(7): 1023 - 1033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. W. Zhou, B. F. C. Kafsack, R. N. Cole, P. Beckett, R. F. Shen, and V. B. Carruthers The Opportunistic Pathogen Toxoplasma gondii Deploys a Diverse Legion of Invasion and Survival Proteins J. Biol. Chem., October 7, 2005; 280(40): 34233 - 34244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Brossier, T. J. Jewett, L. D. Sibley, and S. Urban A spatially localized rhomboid protease cleaves cell surface adhesins essential for invasion by Toxoplasma PNAS, March 15, 2005; 102(11): 4146 - 4151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Wetzel, L. A. Chen, F. A. Ruiz, S. N. J. Moreno, and L. D. Sibley Calcium-mediated protein secretion potentiates motility in Toxoplasma gondii J. Cell Sci., November 15, 2004; 117(24): 5739 - 5748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. W. Zhou, M. J. Blackman, S. A. Howell, and V. B. Carruthers Proteomic Analysis of Cleavage Events Reveals a Dynamic Two-step Mechanism for Proteolysis of a Key Parasite Adhesive Complex Mol. Cell. Proteomics, June 1, 2004; 3(6): 565 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. D. Sibley Intracellular Parasite Invasion Strategies Science, April 9, 2004; 304(5668): 248 - 253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Jewett and L. D. Sibley The Toxoplasma Proteins MIC2 and M2AP Form a Hexameric Complex Necessary for Intracellular Survival J. Biol. Chem., March 5, 2004; 279(10): 9362 - 9369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Lovett and L. D. Sibley Intracellular calcium stores in Toxoplasma gondii govern invasion of host cells J. Cell Sci., July 15, 2003; 116(14): 3009 - 3016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Howell, I. Well, S. L. Fleck, C. Kettleborough, C. R. Collins, and M. J. Blackman A Single Malaria Merozoite Serine Protease Mediates Shedding of Multiple Surface Proteins by Juxtamembrane Cleavage J. Biol. Chem., June 20, 2003; 278(26): 23890 - 23898. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
