Calcium modulates promoter occupancy by the entamoeba histolytica  Ca2+-binding transcription factor URE3-BP

doi:10.1074/jbc.M211271200

QUICK SEARCH:

	Author:		Keyword(s):
Year:		Vol:		Page:

A more recent version of this article appeared on February 7, 2003

This Article

	Full Text (Accepted Manuscript)
	All Versions of this Article: 278/7/4646 most recent M211271200v1
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Email this article to a friend
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager


Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Gilchrist, C. A.
	Articles by Petri, W. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Gilchrist, C. A.
	Articles by Petri, W. A., Jr

Social Bookmarking

	What's this?

Papers In Press, published online ahead of print December 3, 2002
J. Biol. Chem, 10.1074/jbc.M211271200
Submitted on November 4, 2002
Revised on December 2, 2002
Accepted on December 3, 2002

Calcium modulates promoter occupancy by the entamoeba histolytica Ca2+-binding transcription factor URE3-BP

Carol A. Gilchrist, Megan Leo, C. Genghis Line, Barbara J. Mann, and William A. Petri, Jr

Department of Internal Medicine, University of Virginia, Charlottesville, VA 22908

Corresponding Author: wap3g{at}virginia.edu

The Entamoeba histolytica URE3-BP protein binds to the upstream regulatory element 3 (URE3)i sequence of the Gal/GalNAc lectin hgl5 and ferredoxin 1 (fdx) gene promoters. This binding can be inhibited in vitro by addition of calcium. Two EF-hand motifs, which are associated with the ability to bind calcium, are present in the amino acid sequence of URE3-BP. Mutation of the second EF hand motif in URE3-BP resulted in the loss of calcium inhibition of DNA binding as monitored by electrophoretic mobility shift assay. Chromatin immunoprecipitation assays revealed that URE3-BP was physically bound to the hgl5 and fdx promoters in vivo. Parasite intracellular calcium concentrations were altered by changes in extracellular calcium. Promoter occupancy was lost when intracellular calcium levels were increased by coordinate increases in extracellular calcium. Increased intracellular calcium also resulted in decreased levels of URE3BP mRNA. Together these results demonstrate that changes in extracellular calcium result in changes in URE3BP mRNA and in the ability of URE3-BP to bind to URE3-containing promoters. Modulation of URE3-BP by calcium may represent an important mechanism of control of gene expression in E. histolytica.

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

J. A. Hackney, G. M. Ehrenkaufer, and U. Singh
Identification of putative transcriptional regulatory networks in Entamoeba histolytica using Bayesian inference
Nucleic Acids Res., April 1, 2007; 35(7): 2141 - 2152.
[Abstract] [Full Text] [PDF]

A. Mehra, J. Fredrick, W. A. Petri Jr., S. Bhattacharya, and A. Bhattacharya
Expression and Function of a Family of Transmembrane Kinases from the Protozoan Parasite Entamoeba histolytica
Infect. Immun., September 1, 2006; 74(9): 5341 - 5351.
[Abstract] [Full Text] [PDF]

P. Chakrabarty, D. K. Sethi, N. Padhan, K. J. Kaur, D. M. Salunke, S. Bhattacharya, and A. Bhattacharya
Identification and Characterization of EhCaBP2: A SECOND MEMBER OF THE CALCIUM-BINDING PROTEIN FAMILY OF THE PROTOZOAN PARASITE ENTAMOEBA HISTOLYTICA
J. Biol. Chem., March 26, 2004; 279(13): 12898 - 12908.
[Abstract] [Full Text] [PDF]

All ASBMB Journals	Molecular and Cellular Proteomics
Journal of Lipid Research	ASBMB Today

				A. Mehra, J. Fredrick, W. A. Petri Jr., S. Bhattacharya, and A. Bhattacharya Expression and Function of a Family of Transmembrane Kinases from the Protozoan Parasite Entamoeba histolytica Infect. Immun., September 1, 2006; 74(9): 5341 - 5351. [Abstract] [Full Text] [PDF]

				P. Chakrabarty, D. K. Sethi, N. Padhan, K. J. Kaur, D. M. Salunke, S. Bhattacharya, and A. Bhattacharya Identification and Characterization of EhCaBP2: A SECOND MEMBER OF THE CALCIUM-BINDING PROTEIN FAMILY OF THE PROTOZOAN PARASITE ENTAMOEBA HISTOLYTICA J. Biol. Chem., March 26, 2004; 279(13): 12898 - 12908. [Abstract] [Full Text] [PDF]