| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print March 6, 2003
Institut de Microbiologie Fondamentale, Université de Lausanne, Lausanne, Vaud 1015
Corresponding Author: Dieter.Haas{at}imf.unil.ch
In Pseudomonas aeruginosa, the extracellular metabolite and siderophore pyochelin is synthesized from two major precursors, chorismate and L-cysteine, via salicylate as an intermediate. The regulatory role of isochorismate synthase, the first enzyme in the pyochelin biosynthetic pathway, was studied. This enzyme is encoded by pchA, the last gene in the pchDCBA operon. The PchA protein was purified to apparent electrophoretic homogeneity from a PchA-overexpressing P. aeruginosa strain. The native enzyme was a 52 kDa monomer in solution and its activity strictly depended on Mgm. At pH 7.0, the optimum, a Km = 4.5
J. Biol. Chem, 10.1074/jbc.M212324200
Submitted on December 4, 2002
Revised on February 28, 2003
Accepted on March 6, 2003
Isochorismate synthase (PchA), the first and rate-limiting enzyme in salicylate biosynthesis of Pseudomonas aeruginosa
M and a kcat = 43.1 min-1 were determined for chorismate. No feedback inhibitors or other allosteric effectors were found. The intracellular PchA concentration critically determined the rate of salicylate formation both in vitro and in vivo. In cultures grown in iron-limiting media to high cell densities, overexpression of the pchA gene resulted in overproduction of salicylate as well as in enhanced pyochelin formation. From this work and earlier studies it is proposed that one important factor influencing the flux through the pyochelin biosynthetic pathway is the PchA concentration, which is determined at a transcriptional level, with pyochelin acting as a positive signal and iron as a negative signal.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. G. Van Lanen, S. Lin, and B. Shen Biosynthesis of the enediyne antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism PNAS, January 15, 2008; 105(2): 494 - 499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. A. Youard, G. L. A. Mislin, P. A. Majcherczyk, I. J. Schalk, and C. Reimmann Pseudomonas fluorescens CHA0 Produces Enantio-pyochelin, the Optical Antipode of the Pseudomonas aeruginosa Siderophore Pyochelin J. Biol. Chem., December 7, 2007; 282(49): 35546 - 35553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Michel, A. Bachelard, and C. Reimmann Ferripyochelin uptake genes are involved in pyochelin-mediated signalling in Pseudomonas aeruginosa Microbiology, May 1, 2007; 153(5): 1508 - 1518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Strawn, S. K. Marr, K. Inoue, N. Inada, C. Zubieta, and M. C. Wildermuth Arabidopsis Isochorismate Synthase Functional in Pathogen-induced Salicylate Biosynthesis Exhibits Properties Consistent with a Role in Diverse Stress Responses J. Biol. Chem., February 23, 2007; 282(8): 5919 - 5933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zaitseva, J. Lu, K. L. Olechoski, and A. L. Lamb Two Crystal Structures of the Isochorismate Pyruvate Lyase from Pseudomonas aeruginosa J. Biol. Chem., November 3, 2006; 281(44): 33441 - 33449. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Harrison, M. Yu, T. Gardenborg, M. Middleditch, R. J. Ramsay, E. N. Baker, and J. S. Lott The Structure of MbtI from Mycobacterium tuberculosis, the First Enzyme in the Biosynthesis of the Siderophore Mycobactin, Reveals It To Be a Salicylate Synthase. J. Bacteriol., September 1, 2006; 188(17): 6081 - 6091. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bultreys, I. Gheysen, and E. de Hoffmann Yersiniabactin Production by Pseudomonas syringae and Escherichia coli, and Description of a Second Yersiniabactin Locus Evolutionary Group. Appl. Envir. Microbiol., June 1, 2006; 72(6): 3814 - 3825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Kerbarh, A. Ciulli, N. I. Howard, and C. Abell Salicylate Biosynthesis: Overexpression, Purification, and Characterization of Irp9, a Bifunctional Salicylate Synthase from Yersinia enterocolitica J. Bacteriol., August 1, 2005; 187(15): 5061 - 5066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Ogawa, N. Nakajima, T. Sano, M. Tamaoki, M. Aono, A. Kubo, M. Kanna, M. Ioki, H. Kamada, and H. Saji Salicylic Acid Accumulation Under O3 Exposure is Regulated by Ethylene in Tobacco Plants Plant Cell Physiol., July 1, 2005; 46(7): 1062 - 1072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Reimmann, H. M. Patel, C. T. Walsh, and D. Haas PchC Thioesterase Optimizes Nonribosomal Biosynthesis of the Peptide Siderophore Pyochelin in Pseudomonas aeruginosa J. Bacteriol., October 1, 2004; 186(19): 6367 - 6373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Firoved, S. R. Wood, W. Ornatowski, V. Deretic, and G. S. Timmins Microarray Analysis and Functional Characterization of the Nitrosative Stress Response in Nonmucoid and Mucoid Pseudomonas aeruginosa J. Bacteriol., June 15, 2004; 186(12): 4046 - 4050. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
