JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on March 21, 2003
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
278/13/11480    most recent
M300080200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kumar, A.
Right arrow Articles by Reddy, E. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kumar, A.
Right arrow Articles by Reddy, E. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print January 13, 2003
J. Biol. Chem, 10.1074/jbc.M300080200
Submitted on January 5, 2003
Revised on January 13, 2003
Accepted on January 13, 2003

C-Myc is essential but not sufficient for c-Myb-mediated block of granulocytic differentiation

Atul Kumar, Clement M. Lee, and E. Premkumar Reddy

Fels Research Institute, Temple University School of Medicine, Philadelphia, Pennsylvania 19140

Corresponding Author: reddy{at}unix.temple.edu

The c-myb proto-oncogene plays a central role in hematopoiesis and encodes a major translational product of 75 kDa. c-Myb is highly expressed in immature hematopoietic cells and its expression is down-regulated during terminal differentiation. Deregulated expression of c-Myb has been shown to block terminal differentiation of hematopoietic cells. Here we have studied the mechanism of action and the nature of target genes through which c-Myb mediates the block in differentiation of 32Dcl3 murine myeloid cells. We show that the ectopic over-expression of c-Myb in 32Dcl3 cells results in the over-expression of c-Myc. However, enforced expression of c-Myc in 32Dcl3 cells did not alter the normal pattern of differentiation. In addition, expression of dominant negative mutants of c-Myc relieved c-Myb-mediated block in differentiation. These results lead us to conclude that c-myc is a target gene of c-Myb and activation of the c-myc gene is a necessary event in Myb-mediated transformation. However, c-Myc expression alone is inadequate to elicit the phenotypic effects seen with Myb-mediated block in differentiation of myeloid cells, suggesting that activation of additional transcriptional targets by c-Myb plays a critical role in this process.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Stem CellsHome page
K. O. Gudmundsson, L. Thorsteinsson, O. E. Sigurjonsson, J. R. Keller, K. Olafsson, T. Egeland, S. Gudmundsson, and T. Rafnar
Gene Expression Analysis of Hematopoietic Progenitor Cells Identifies Dlg7 as a Potential Stem Cell Gene
Stem Cells, June 1, 2007; 25(6): 1498 - 1506.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Kumar, S. J. Baker, C. M. Lee, and E. P. Reddy
Molecular Mechanisms Associated with the Regulation of Apoptosis by the Two Alternatively Spliced Products of c-Myb
Mol. Cell. Biol., September 15, 2003; 23(18): 6631 - 6645.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.