| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print March 5, 2003
Physiology and Biophysics, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854
Corresponding Author: fanhu{at}umdnj.edu
Tumor necrosis factor-
J. Biol. Chem, 10.1074/jbc.M300331200
Submitted on January 12, 2003
Revised on March 5, 2003
Accepted on March 4, 2003
Characterization of growth factor-induced serine phosphorylation of tumor necrosis factor-
converting enzyme (TACE) and of an alternatively translated polypeptide
converting enzyme (TACE) is a prototype member of the adamalysin family of transmembrane metalloproteases that effects ectodomain cleavage and release of many transmembrane proteins, including TGF-. Growth factors that act through tyrosine kinase receptors, as well as other stimuli, induce shedding through activation of the Erk MAP kinase pathway without the need of new protein synthesis. How MAP kinase regulates shedding by TACE is not known. We now report that the cytoplasmic domain of TACE is phosphorylated in response to growth factor stimulation. We also identified a naturally expressed smaller polypeptide corresponding to most of the cytoplasmic domain of TACE. This protein, which we named SPRACT, is derived through alternative translation of the TACE coding sequence, and is, similarly to TACE, phosphorylated in response to growth factor and PMA stimulation. Phosphoamino acid analysis revealed that growth factor-induced phosphorylation of TACE occurs only on serine, and not on threonine or tyrosine. Tryptic mapping experiments coupled with site-directed mutagenesis identified Ser819 as the major target of growth factor-induced phosphorylation, whereas Ser791 undergoes dephosphorylation in response to growth factor stimulation. The phosphorylation of Ser819, but not the dephosphorylation of Ser791, depends on activation of the Erk MAP kinase pathway. Increased SPRACT expression or mutation of the TACE cytoplasmic domain to inactivate growth factor-induced phosphorylation did not detectably affect growth factor-induced shedding of transmembrane TGF- by TACE. The roles of SPRACT and the cytoplasmic phosphorylation of TACE remain to be defined.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. E. Wang, B. Xiang, M. Guix, M. G. Olivares, J. Parker, C. H. Chung, A. Pandiella, and C. L. Arteaga Transforming Growth Factor {beta} Engages TACE and ErbB3 To Activate Phosphatidylinositol-3 Kinase/Akt in ErbB2-Overexpressing Breast Cancer and Desensitizes Cells to Trastuzumab Mol. Cell. Biol., September 15, 2008; 28(18): 5605 - 5620. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Montero, R. Rodriguez-Barrueco, A. Ocana, E. Diaz-Rodriguez, A. Esparis-Ogando, and A. Pandiella Neuregulins and Cancer Clin. Cancer Res., June 1, 2008; 14(11): 3237 - 3241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Findley, M. J. Cudmore, A. Ahmed, and C. D. Kontos VEGF Induces Tie2 Shedding via a Phosphoinositide 3-Kinase/Akt Dependent Pathway to Modulate Tie2 Signaling Arterioscler. Thromb. Vasc. Biol., December 1, 2007; 27(12): 2619 - 2626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Keates, X. Han, C. P. Kelly, and A. C. Keates Macrophage-Inflammatory Protein-3{alpha} Mediates Epidermal Growth Factor Receptor Transactivation and ERK1/2 MAPK Signaling in Caco-2 Colonic Epithelial Cells via Metalloproteinase-Dependent Release of Amphiregulin J. Immunol., June 15, 2007; 178(12): 8013 - 8021. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Alfa Cisse, C. Sunyach, B. E. Slack, A. Fisher, B. Vincent, and F. Checler M1 and M3 Muscarinic Receptors Control Physiological Processing of Cellular Prion by Modulating ADAM17 Phosphorylation and Activity J. Neurosci., April 11, 2007; 27(15): 4083 - 4092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. H. Heijink, P. Marcel Kies, A. J. M. van Oosterhout, D. S. Postma, H. F. Kauffman, and E. Vellenga Der p, IL-4, and TGF-beta Cooperatively Induce EGFR-Dependent TARC Expression in Airway Epithelium Am. J. Respir. Cell Mol. Biol., March 1, 2007; 36(3): 351 - 359. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zhan, B. Jin, S.-E. Chen, J. M. Reecy, and Y.-P. Li TACE release of TNF-{alpha} mediates mechanotransduction-induced activation of p38 MAPK and myogenesis J. Cell Sci., February 15, 2007; 120(4): 692 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Horiuchi, S. Le Gall, M. Schulte, T. Yamaguchi, K. Reiss, G. Murphy, Y. Toyama, D. Hartmann, P. Saftig, and C. P. Blobel Substrate Selectivity of Epidermal Growth Factor-Receptor Ligand Sheddases and their Regulation by Phorbol Esters and Calcium Influx Mol. Biol. Cell, January 1, 2007; 18(1): 176 - 188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ohtsu, P. J. Dempsey, G. D. Frank, E. Brailoiu, S. Higuchi, H. Suzuki, H. Nakashima, K. Eguchi, and S. Eguchi ADAM17 Mediates Epidermal Growth Factor Receptor Transactivation and Vascular Smooth Muscle Cell Hypertrophy Induced by Angiotensin II Arterioscler. Thromb. Vasc. Biol., September 1, 2006; 26(9): e133 - e137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ohtsu, P. J. Dempsey, and S. Eguchi ADAMs as mediators of EGF receptor transactivation by G protein-coupled receptors Am J Physiol Cell Physiol, July 1, 2006; 291(1): C1 - C10. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Balakrishnan, B. Patel, S. A. Sieber, D. Chen, N. Pachikara, G. Zhong, B. F. Cravatt, and H. Fan Metalloprotease Inhibitors GM6001 and TAPI-0 Inhibit the Obligate Intracellular Human Pathogen Chlamydia trachomatis by Targeting Peptide Deformylase of the Bacterium J. Biol. Chem., June 16, 2006; 281(24): 16691 - 16699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Zhang, S. M. Thomas, V. W. Y. Lui, S. Xi, J. M. Siegfried, H. Fan, T. E. Smithgall, G. B. Mills, and J. R. Grandis Phosphorylation of TNF-{alpha} converting enzyme by gastrin-releasing peptide induces amphiregulin release and EGF receptor activation PNAS, May 2, 2006; 103(18): 6901 - 6906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Sternlicht, S. W. Sunnarborg, H. Kouros-Mehr, Y. Yu, D. C. Lee, and Z. Werb Mammary ductal morphogenesis requires paracrine activation of stromal EGFR via ADAM17-dependent shedding of epithelial amphiregulin Development, September 1, 2005; 132(17): 3923 - 3933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, R. F. Schwabe, T. DeVries-Seimon, P. M. Yao, M.-C. Gerbod-Giannone, A. R. Tall, R. J. Davis, R. Flavell, D. A. Brenner, and I. Tabas Free Cholesterol-loaded Macrophages Are an Abundant Source of Tumor Necrosis Factor-{alpha} and Interleukin-6: MODEL OF NF-{kappa}B- AND MAP KINASE-DEPENDENT INFLAMMATION IN ADVANCED ATHEROSCLEROSIS J. Biol. Chem., June 10, 2005; 280(23): 21763 - 21772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Soond, B. Everson, D. W. H. Riches, and G. Murphy ERK-mediated phosphorylation of Thr735 in TNF{alpha}-converting enzyme and its potential role in TACE protein trafficking J. Cell Sci., June 1, 2005; 118(11): 2371 - 2380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Levine Mechanisms of Soluble Cytokine Receptor Generation J. Immunol., November 1, 2004; 173(9): 5343 - 5348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hagan, A. Yacoub, and P. Dent Ionizing Radiation Causes a Dose-Dependent Release of Transforming Growth Factor {alpha} In vitro from Irradiated Xenografts and during Palliative Treatment of Hormone-Refractory Prostate Carcinoma Clin. Cancer Res., September 1, 2004; 10(17): 5724 - 5731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li and H. Fan Loss of Ectodomain Shedding Due to Mutations in the Metalloprotease and Cysteine-rich/Disintegrin Domains of the Tumor Necrosis Factor-{alpha} Converting Enzyme (TACE) J. Biol. Chem., June 25, 2004; 279(26): 27365 - 27375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. M. Fischer, S. Hart, A. Gschwind, N. Prenzel, and A. Ullrich Oxidative and Osmotic Stress Signaling in Tumor Cells Is Mediated by ADAM Proteases and Heparin-Binding Epidermal Growth Factor Mol. Cell. Biol., June 15, 2004; 24(12): 5172 - 5183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. W. Park, T. J. Foster, E. Nishi, S. J. Duncan, M. Klagsbrun, and Y. Chen Activation of Syndecan-1 Ectodomain Shedding by Staphylococcus aureus {alpha}-Toxin and {beta}-Toxin J. Biol. Chem., January 2, 2004; 279(1): 251 - 258. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
