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M301041200v1
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Papers In Press, published online ahead of print July 8, 2003
J. Biol. Chem, 10.1074/jbc.M301041200
Submitted on January 30, 2003
Revised on July 1, 2003
Accepted on July 8, 2003

Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA

Jose Gallego, Jane Greatorex, Hui Zhang, Bin Yang, Shyamala Arunachalam, Jianhua Fang, John Seamons, Susan Lea, Roger Pomerantz, and Andrew M.L. Lever

Medicine, University of Cambridge, Cambridge CB2 2QQ

Corresponding Author: amll1{at}medschl.cam.ac.uk

The leader RNA sequence of human immunodeficiency virus type 1 (HIV-1) consists of a complex series of stem loop structures that are critical for viral replication. Three dimensional structural analysis by NMR of one of these structures, the SL-1 stem loop of the packaging signal region, revealed a highly conserved purine rich loop with near identity in structure to the Rev binding loop of the Rev response element (RRE). Using band shift assays, surface plasmon resonance and further NMR analysis we demonstrate that this loop binds Rev. HIV-1 appears to have a second Rev binding site close to the major splice donor site that may have an additional role in the viral life-cycle.


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