JBC PeproTech; Our Business is Cytokines!

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on June 27, 2003
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
278/27/24600    most recent
M303424200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cheng, H.-C.
Right arrow Articles by Pauli, B. U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, H.-C.
Right arrow Articles by Pauli, B. U.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print April 25, 2003
J. Biol. Chem, 10.1074/jbc.M303424200
Submitted on April 2, 2003
Revised on April 18, 2003
Accepted on April 25, 2003

A novel consensus motif in fibronectin mediates dipeptidyl peptidase IV adhesion and metastasis

Hung-Chi Cheng, Mossaad Abdel-Ghany, and Bendicht U. Pauli

Molecular Medicine, Cancer Biology Laboratories, Cornell University, College of Veterinary Medicine, Ithaca, NY 14853-6401

Corresponding Author: bup1{at}cornell.edu

Lung endothelial dipeptidyl peptidase IV (DPPIV/CD26) is a vascular address for cancer cells decorated with polymeric cell-surface fibronectin (polyFN). Here, we identify the DPPIV-binding sites of FN and examined the effect of binding site-peptides on DPPIV/polyFN adhesion and metastasis. Using proteolytic fragments and maltose-binding protein (MBP)-fusion proteins that together span the full-length of FN, we found DPPIV-binding sites in the 13th, 14th, and 15th type III repeats (FNIII13, FNIII14, FNIII15). DPPIV-binding was mediated by the consensus motif T(I/L)TGLX(P/R)G(T/V)X and was confirmed by swapping this motif in FNIII13, FNIII14, and FNIII15 with the corresponding region in FNIII12, which did not bind DPPIV. DPPIV-binding was lost in swapped FNIII13, FNIII14, and FNIII15 and gained in swapped FNIII12 [FNIII12(14)]. Peptides containing the DPPIV-binding domain of FNIII14 block the DPPIV/polyFN adhesion and impede pulmonary metastasis. This study adds to the classes of cell surface adhesion receptors for FN and will help in a further characterization of the functional implication of the DPPIV/polyFN adhesion in metastasis and, possibly, in cell-mediated immunity involving DPPIV-expressing lymphocytes.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
L. Huang, H.-C. Cheng, R. Isom, C.-S. Chen, R. A. Levine, and B. U. Pauli
Protein Kinase C{epsilon} Mediates Polymeric Fibronectin Assembly on the Surface of Blood-borne Rat Breast Cancer Cells to Promote Pulmonary Metastasis
J. Biol. Chem., March 21, 2008; 283(12): 7616 - 7627.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
T. Inamoto, T. Yamada, K. Ohnuma, S. Kina, N. Takahashi, T. Yamochi, S. Inamoto, Y. Katsuoka, O. Hosono, H. Tanaka, et al.
Humanized Anti-CD26 Monoclonal Antibody as a Treatment for Malignant Mesothelioma Tumors
Clin. Cancer Res., July 15, 2007; 13(14): 4191 - 4200.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
Y. Shimomura, H. Ando, K. Furugori, H. Kajiyama, M. Suzuki, A. Iwase, S. Mizutani, and F. Kikkawa
Possible involvement of crosstalk cell-adhesion mechanism by endometrial CD26/dipeptidyl peptidase IV and embryonal fibronectin in human blastocyst implantation
Mol. Hum. Reprod., August 1, 2006; 12(8): 491 - 495.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
T. Inamoto, T. Yamochi, K. Ohnuma, S. Iwata, S. Kina, S. Inamoto, M. Tachibana, Y. Katsuoka, N. H. Dang, and C. Morimoto
Anti-CD26 Monoclonal Antibody-Mediated G1-S Arrest of Human Renal Clear Cell Carcinoma Caki-2 Is Associated with Retinoblastoma Substrate Dephosphorylation, Cyclin-Dependent Kinase 2 Reduction, p27kip1 Enhancement, and Disruption of Binding to the Extracellular Matrix.
Clin. Cancer Res., June 1, 2006; 12(11): 3470 - 3477.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. Sato, T. Yamochi, T. Yamochi, U. Aytac, K. Ohnuma, K. S. McKee, C. Morimoto, and N. H. Dang
CD26 Regulates p38 Mitogen-Activated Protein Kinase-Dependent Phosphorylation of Integrin {beta}1, Adhesion to Extracellular Matrix, and Tumorigenicity of T-Anaplastic Large Cell Lymphoma Karpas 299
Cancer Res., August 1, 2005; 65(15): 6950 - 6956.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Abdel-Ghany, H.-C. Cheng, R. C. Elble, H. Lin, J. DiBiasio, and B. U. Pauli
The Interacting Binding Domains of the {beta}4 Integrin and Calcium-activated Chloride Channels (CLCAs) in Metastasis
J. Biol. Chem., December 5, 2003; 278(49): 49406 - 49416.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.