JBC Focus on PI3-Kinase with Echelon

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on July 3, 2003
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
278/28/26249    most recent
M304459200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Prunier, C.
Right arrow Articles by Atfi, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Prunier, C.
Right arrow Articles by Atfi, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print May 5, 2003
J. Biol. Chem, 10.1074/jbc.M304459200
Submitted on April 29, 2003
Revised on May 5, 2003
Accepted on May 5, 2003

The oncoprotein Ski acts as an antagonist of TGF-b signaling by suppressing Smad2 phosphorylation

Céline Prunier, Marcia Pessah, Nathalie Ferrand, Su Ryeon Seo, Philip Howe, and Azeddine Atfi

INSERM U482, INSERM, Hôpital St-Antoine, Paris 75571

Corresponding Author: atfi{at}pop.st-antoine.inserm.fr

The phosphorylation of Smad2 and Smad3 by the TGF-b-activated receptor kinases and their subsequent heterodimerization with Smad4 and translocation to the nucleus form the basis for a model how Smad proteins work to transmit TGF-b signals. The transcriptional activity of Smad2/Smad4 or Smad3/Smad4 complexes can be limited by the corepressor Ski, which is believed to interact with Smad complexes on TGF-b-responsive promoters and represses their ability to activate TGF-b target genes by assembling on DNA a repressor complex containing histone deacetylase. Here we show that Ski can block TGF-b signaling by interfering with the phosphorylation of Smad2 and Smad3 by the activated TGF-b type I receptor. Furthermore, we demonstrate that overexpression of Ski induces the assembly of Smad2/Smad4 and Smad3/Smad4 complexes independent of TGF-b signaling. The ability of Ski to engage Smad proteins in non-productive complexes provides new insights into the molecular mechanism used by Ski for disabling TGF-b signaling.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 GENES CELLSHome page
M. Nagata, K. Goto, S. Ehata, N. Kobayashi, M. Saitoh, H. Miyoshi, T. Imamura, K. Miyazawa, and K. Miyazono
Nuclear and cytoplasmic c-Ski differently modulate cellular functions
Genes Cells, November 1, 2006; 11(11): 1267 - 1280.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Chang, A. H. Brivanlou, and R. M. Harland
Function of the Two Xenopus Smad4s in Early Frog Development
J. Biol. Chem., October 13, 2006; 281(41): 30794 - 30803.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
R. Tan, J. Zhang, X. Tan, X. Zhang, J. Yang, and Y. Liu
Downregulation of SnoN Expression in Obstructive Nephropathy Is Mediated by an Enhanced Ubiquitin-Dependent Degradation
J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2781 - 2791.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. G. Denissova and F. Liu
Repression of Endogenous Smad7 by Ski
J. Biol. Chem., July 2, 2004; 279(27): 28143 - 28148.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. C. McKarns, R. H. Schwartz, and N. E. Kaminski
Smad3 Is Essential for TGF-{beta}1 to Suppress IL-2 Production and TCR-Induced Proliferation, but Not IL-2-Induced Proliferation
J. Immunol., April 1, 2004; 172(7): 4275 - 4284.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Takeda, M. Mizuide, M. Oka, T. Watabe, H. Inoue, H. Suzuki, T. Fujita, T. Imamura, K. Miyazono, and K. Miyazawa
Interaction with Smad4 Is Indispensable for Suppression of BMP Signaling by c-Ski
Mol. Biol. Cell, March 1, 2004; 15(3): 963 - 972.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.