| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print July 14, 2003
Institute of Biomedical and Biomolecular Sciences, University of Portsmouth, Portsmouth, Hants PO1 2DT
Corresponding Author: colyn.crane-robinson{at}port.ac.uk
Acetylation of histones H4 and H3 targeted to promoters/enhancers is linked to the activation of transcription, whilst widespread, long range acetylation of the same histones has been linked to the requirement for open chromatin at transcriptionally active loci and at regions of V(D)J recombination. Using affinity-purified polyclonal antibodies to tetra/tri-acetylated histone H2B in chromatin immuno-precipitation (ChIP) assays with mononucleosomes from 15-day chicken embryo erythrocytes, a high resolution distribution of H2B acetylation has been determined and compared to that of H4 and H3 at the same genes/loci. At the
J. Biol. Chem, 10.1074/jbc.M305822200
Submitted on June 3, 2003
Revised on July 14, 2003
Accepted on July 14, 2003
Acetylation of histone H2B mirrors that of H4 and H3 at the chicken
-globin locus but not at housekeeping genes
-globin locus the H2B acetylation is high throughout and in general mirrors that of H3 and H4, consistent with the observation of co-precipitation of hyperacetylated H4 together with the hyperacetylated H2B. In contrast, at the weakly expressed genes GAPDH and Gas41 (housekeeping) and carbonic anhydrase (tissue specific), very little or no hyperacetylated H2B was found despite the presence of acetylated H4 and H3 at their promoters and proximal transcribed sequences. At the inactive lysozyme and ovalbumin genes essentially no acetylation of H2B, H3 or H4 was observed. Acetylation of H2B appears to be principally a feature of only the most actively transcribed genes/loci.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J.-M. Sun, H. Y. Chen, P. S. Espino, and J. R. Davie Phosphorylated serine 28 of histone H3 is associated with destabilized nucleosomes in transcribed chromatin Nucleic Acids Res., October 8, 2007; 35(19): 6640 - 6647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. Myers, P. Lefevre, E. Mantouvalou, K. Bruce, C. Lacroix, C. Bonifer, A. W. Thorne, and C. Crane-Robinson Developmental activation of the lysozyme gene in chicken macrophage cells is linked to core histone acetylation at its enhancer elements Nucleic Acids Res., September 1, 2006; 34(14): 4025 - 4035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Thambirajah, D. Dryhurst, T. Ishibashi, A. Li, A. H. Maffey, and J. Ausio H2A.Z Stabilizes Chromatin in a Way That Is Dependent on Core Histone Acetylation J. Biol. Chem., July 21, 2006; 281(29): 20036 - 20044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Bonenfant, M. Coulot, H. Towbin, P. Schindler, and J. van Oostrum Characterization of Histone H2A and H2B Variants and Their Post-translational Modifications by Mass Spectrometry Mol. Cell. Proteomics, March 1, 2006; 5(3): 541 - 552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bruce, F. A. Myers, E. Mantouvalou, P. Lefevre, I. Greaves, C. Bonifer, D. J. Tremethick, A. W. Thorne, and C. Crane-Robinson The replacement histone H2A.Z in a hyperacetylated form is a feature of active genes in the chicken Nucleic Acids Res., October 4, 2005; 33(17): 5633 - 5639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Fu, R. A. McKnight, X. Yu, L. Wang, C. W. Callaway, and R. H. Lane Uteroplacental insufficiency induces site-specific changes in histone H3 covalent modifications and affects DNA-histone H3 positioning in day 0 IUGR rat liver Physiol Genomics, December 15, 2004; 20(1): 108 - 116. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
