| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print January 10, 2004
Molecular Mechanisms of Mycobacterial Infections, Institut de Pharmacologie et Biologie Structurale, Toulouse 31077
Corresponding Author: daffe{at}ipbs.fr
The attenuated strain of Mycobacterium bovis, Bacille Calmette-Guérin (BCG), used worldwide to prevent tuberculosis and leprosy is also clinically used as an immunotherapeutic agent against superficial bladder cancer. An anti-tumor polysaccharide has been isolated from the boiling-water extract of the Tice substrain of BCG and tentatively characterized as consisting primarily of repeating units of 6-linked-glucosyl residues. M. tuberculosis and other mycobacterial species produce a glycogen-like alpha-glucan, composed of repeating units of 4-linked-glucosyl residues substituted at some positions 6 by short oligoglucosyl units, that also exhibits an anti-tumor activity. Therefore, the impression prevails that mycobacteria synthesize different types of anti-neoplastic glucans or, alternatively, the BCG substrains are singular in producing a unique type of glucan that may confer to them their immunotherapeutic property. The present study addresses this question through the comparative analysis of alpha-glucans purified from the extracellular materials and boiling-water extracts of three vaccine substrains. The polysaccharides were purified and their structural features were established by mono-and two-dimensional NMR spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of the enzymatic and chemical degradation products of the purified compounds. The glucans isolated by the two methods from the three substrains of BCG were shown to exhibit identical structural features shared with the glycogen-like alpha-glucan of M. tuberculosis and other mycobacteria. Incidentally, we observed an occasional release of dextrans from Sephadex columns that may explain the reported occurrence of 6-substituted-alpha-glucans in mycobacteria.
J. Biol. Chem, 10.1074/jbc.M308908200
Submitted on August 12, 2003
Revised on January 9, 2004
Accepted on January 9, 2004
Revisiting the structure of the anti-neoplastic glucans of mycobacterium bovis bacille calmette-gu
rin analysis of the different types of glucans produced by the vaccine substrains
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Dinadayala, T. Sambou, M. Daffe, and A. Lemassu Comparative structural analyses of the {alpha}-glucan and glycogen from Mycobacterium bovis Glycobiology, July 1, 2008; 18(7): 502 - 508. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Stadthagen, T. Sambou, M. Guerin, N. Barilone, F. Boudou, J. Kordulakova, P. Charles, P. M. Alzari, A. Lemassu, M. Daffe, et al. Genetic Basis for the Biosynthesis of Methylglucose Lipopolysaccharides in Mycobacterium tuberculosis J. Biol. Chem., September 14, 2007; 282(37): 27270 - 27276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Berg, D. Kaur, M. Jackson, and P. J Brennan The glycosyltransferases of Mycobacterium tuberculosis--roles in the synthesis of arabinogalactan, lipoarabinomannan, and other glycoconjugates Glycobiology, June 1, 2007; 17(6): 35R - 56R. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yadav and J. S. Schorey The beta-glucan receptor dectin-1 functions together with TLR2 to mediate macrophage activation by mycobacteria Blood, November 1, 2006; 108(9): 3168 - 3175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. C. B. Bittencourt, R. T. Figueiredo, R. B. da Silva, D. S. Mourao-Sa, P. L. Fernandez, G. L. Sassaki, B. Mulloy, M. T. Bozza, and E. Barreto-Bergter An {alpha}-Glucan of Pseudallescheria boydii Is Involved in Fungal Phagocytosis and Toll-like Receptor Activation J. Biol. Chem., August 11, 2006; 281(32): 22614 - 22623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E.B. Lee, W. Li, D. Chatterjee, and R. E. Lee Rapid structural characterization of the arabinogalactan and lipoarabinomannan in live mycobacterial cells using 2D and 3D HR-MAS NMR: structural changes in the arabinan due to ethambutol treatment and gene mutation are observed Glycobiology, February 1, 2005; 15(2): 139 - 151. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
