| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print October 31, 2003
Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110
Corresponding Author: burgers{at}biochem.wustl.edu
We have carried out a domain analysis of POL32, the third subunit of S. cerevisiae DNA polymerase
J. Biol. Chem, 10.1074/jbc.M310362200
Submitted on September 17, 2003
Revised on October 30, 2003
Accepted on October 30, 2003
The Pol32 subunit of DNA polymerase
contains separable domains for processive replication and PCNA binding
(Pol ). Interactions with POL31, the second subunit of Pol , are specified by the amino terminal 92 amino acids, whereas interactions with the replication clamp PCNA (POL30) reside at the extreme carboxy-terminal region. Pol32 binding, in vivo and in vitro, to the large subunit of DNA polymerase 
, POL1, requires the carboxy-proximal region of Pol32. The amino-terminal region of Pol32 is essential for damage-induced mutagenesis. However, the presence of its carboxy-terminal PCNA-binding domain enhances the efficiency of mutagenesis, particularly at high loads of DNA damage. In vitro, in the absence of effector DNA, the PCNA-binding domain of Pol32 is essential for PCNA-Pol interactions. However, this domain has minimal importance for processive DNA synthesis by the ternary DNA-PCNA-Pol complex. Rather, processivity is determined by PCNA-binding domains located in the Pol3 and/or Pol31 subunits. Using diagnostic PCNA mutants, we showed that during DNA synthesis the carboxy-terminal domain of Pol32 interacts with the carboxy-terminal region of PCNA, whereas interactions of the other subunit(s) of Pol localize largely to a hydrophobic pocket at the interdomain connector loop region of PCNA.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Deem, K. Barker, K. VanHulle, B. Downing, A. Vayl, and A. Malkova Defective Break-Induced Replication Leads to Half-Crossovers in Saccharomyces cerevisiae Genetics, August 1, 2008; 179(4): 1845 - 1860. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. van Welsem, F. Frederiks, K. F. Verzijlbergen, A. W. Faber, Z. W. Nelson, D. A. Egan, D. E. Gottschling, and F. van Leeuwen Synthetic Lethal Screens Identify Gene Silencing Processes in Yeast and Implicate the Acetylated Amino Terminus of Sir3 in Recognition of the Nucleosome Core Mol. Cell. Biol., June 1, 2008; 28(11): 3861 - 3872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Zhuang, R. E. Johnson, L. Haracska, L. Prakash, S. Prakash, and S. J. Benkovic Regulation of polymerase exchange between Pol{eta} and Pol{delta} by monoubiquitination of PCNA and the movement of DNA polymerase holoenzyme PNAS, April 8, 2008; 105(14): 5361 - 5366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Pages, R. E. Johnson, L. Prakash, and S. Prakash Mutational specificity and genetic control of replicative bypass of an abasic site in yeast PNAS, January 29, 2008; 105(4): 1170 - 1175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Masuda, M. Suzuki, J. Piao, Y. Gu, T. Tsurimoto, and K. Kamiya Dynamics of human replication factors in the elongation phase of DNA replication Nucleic Acids Res., November 29, 2007; 35(20): 6904 - 6916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Chilkova, P. Stenlund, I. Isoz, C. M. Stith, P. Grabowski, E.-B. Lundstrom, P. M. Burgers, and E. Johansson The eukaryotic leading and lagging strand DNA polymerases are loaded onto primer-ends via separate mechanisms but have comparable processivity in the presence of PCNA Nucleic Acids Res., October 8, 2007; 35(19): 6588 - 6597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Lee and S. E. Lee Saccharomyces cerevisiae Sae2- and Tel1-Dependent Single-Strand DNA Formation at DNA Break Promotes Microhomology-Mediated End Joining Genetics, August 1, 2007; 176(4): 2003 - 2014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Das-Bradoo, R. M. Ricke, and A.-K. Bielinsky Interaction between PCNA and Diubiquitinated Mcm10 Is Essential for Cell Growth in Budding Yeast. Mol. Cell. Biol., July 1, 2006; 26(13): 4806 - 4817. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Li, B. Xie, Y. Zhou, A. Rahmeh, S. Trusa, S. Zhang, Y. Gao, E. Y. C. Lee, and M. Y. W. T. Lee Functional Roles of p12, the Fourth Subunit of Human DNA Polymerase {delta} J. Biol. Chem., May 26, 2006; 281(21): 14748 - 14755. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Budd, C. C. Reis, S. Smith, K. Myung, and J. L. Campbell Evidence Suggesting that Pif1 Helicase Functions in DNA Replication with the Dna2 Helicase/Nuclease and DNA Polymerase {delta}. Mol. Cell. Biol., April 1, 2006; 26(7): 2490 - 2500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Garg, C. M. Stith, J. Majka, and P. M. J. Burgers Proliferating Cell Nuclear Antigen Promotes Translesion Synthesis by DNA Polymerase {zeta} J. Biol. Chem., June 24, 2005; 280(25): 23446 - 23450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. K. Minesinger and S. Jinks-Robertson Roles of RAD6 Epistasis Group Members in Spontaneous Pol{zeta}-Dependent Translesion Synthesis in Saccharomyces cerevisiae Genetics, April 1, 2005; 169(4): 1939 - 1955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Mitkova, E. E. Biswas-Fiss, and S. B. Biswas Modulation of DNA Synthesis in Saccharomyces cerevisiae Nuclear Extract by DNA Polymerases and the Origin Recognition Complex J. Biol. Chem., February 25, 2005; 280(8): 6285 - 6292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Haracska, N. Acharya, I. Unk, R. E. Johnson, J. Hurwitz, L. Prakash, and S. Prakash A Single Domain in Human DNA Polymerase {iota} Mediates Interaction with PCNA: Implications for Translesion DNA Synthesis Mol. Cell. Biol., February 1, 2005; 25(3): 1183 - 1190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tanaka, G.-H. Ryu, Y.-S. Seo, and S. A. MacNeill Genetics of lagging strand DNA synthesis and maturation in fission yeast: suppression analysis links the Dna2-Cdc24 complex to DNA polymerase {delta} Nucleic Acids Res., December 2, 2004; 32(21): 6367 - 6377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. McCulloch, R. J. Kokoska, O. Chilkova, C. M. Welch, E. Johansson, P. M. J. Burgers, and T. A. Kunkel Enzymatic switching for efficient and accurate translesion DNA replication Nucleic Acids Res., August 27, 2004; 32(15): 4665 - 4675. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
