JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on May 14, 2004
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
279/20/21160    most recent
M312199200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Robert, R.
Right arrow Articles by Becq, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Robert, R.
Right arrow Articles by Becq, F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print March 11, 2004
J. Biol. Chem, 10.1074/jbc.M312199200
Submitted on November 7, 2003
Revised on March 8, 2004
Accepted on March 11, 2004

Regulation of the cystic fibrosis transmembrane conductance regulator channel by beta -adrenergic-agonists and VIP in rat smooth muscle cells and its role in vasorelaxation

Renaud Robert, Vincent Thoreau, Caroline Norez, Anne Cantereau, Alain Kitzis, Yvette Mettey, Christian Rogier, and Frederic Becq

Physiology, UMR 6558 University of Poitiers, POITIERS 86022

Corresponding Author: frederic.becq{at}univ-poitiers.fr

The signalling events that regulate vascular tone include voltage-dependent Ca2+-influx and various ionic channels activity which molecular entities and role are still a matter of debate. Here we show expression of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel in rat aortic smooth muscle cells. Immunoprecipitation and in vitro PKA-phosphorylation shows appearance of mature band C of CFTR. Immunohistochemistry study shows CFTR proteins in smooth muscles of aortic rings but not in skeletal muscles. Using iodide efflux method, a combinaison of agonists and pharmacological agents was used to dissect the function of CFTR. Agonists of the cAMP pathway, the b-adrenergic agonist isoproterenol and the neuropeptide VIP activate CFTR-dependent transport from cells maintained in a high but not low extracellular potassium-rich saline suggesting that depolarization of smooth muscle is critical to CFTR activation. Smooth muscle CFTR possesses all the pharmacological attributes of its epithelial homologous: stimulation by the CFTR pharmacological activators MPB-07 (EC50 = 158 µM) and MPB-91 (EC50 = 20 µM); inhibition by glibenclamide, DPC but not by TS-TM calix[4]arene. Contraction measurements on isolated aortic rings were performed to study the contribution of CFTR to vascular tone. With aortic rings (without endothelium) pre-constricted by high K+ saline or by the a-adrenergic agonist norepinephrine, CFTR activators produced a concentration-dependent relaxation. These results identify for the first time expression and function of CFTR in smooth muscle where it plays an unexpected but fundamental role in the autonomic and hormonal regulation of the vascular tone.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Eur Respir JHome page
R. Robert, J-P. Savineau, C. Norez, F. Becq, and C. Guibert
Expression and function of cystic fibrosis transmembrane conductance regulator in rat intrapulmonary arteries
Eur. Respir. J., November 1, 2007; 30(5): 857 - 864.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
R. Robert, C. Norez, and F. Becq
Disruption of CFTR chloride channel alters mechanical properties and cAMP-dependent Cl- transport of mouse aortic smooth muscle cells
J. Physiol., October 15, 2005; 568(2): 483 - 495.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Rene, M. Taulan, F. Iral, J. Doudement, A. L'Honore, C. Gerbon, J. Demaille, M. Claustres, and M.-C. Romey
Binding of serum response factor to cystic fibrosis transmembrane conductance regulator CArG-like elements, as a new potential CFTR transcriptional regulation pathway
Nucleic Acids Res., September 16, 2005; 33(16): 5271 - 5290.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.