| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print June 8, 2004
Medicine Dept., David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1786
Corresponding Author: erozengurt{at}mednet.ucla.edu
Protein kinase D (PKD) is a serine/threonine protein kinase activated by G protein-coupled receptor (GPCR) agonists through an incompletely characterized mechanism that includes its reversible plasma membrane translocation and activation loop phosphorylation via a protein kinase C (PKC)-dependent pathway. In order to gain a better understanding of the mechanism regulating the activation of PKD in response to GPCR stimulation, we investigated the role of its rapid membrane translocation on its activation loop phosphorylation and identified the endogenous PKC isozyme that mediates that event in vivo. We had found that the activation loop of a PKD mutant, with reduced affinity for diacylglycerol and phorbol esters, was only phosphorylated upon its plasma membrane association. We also found that the activation loop phosphorylation and rapid plasma membrane dissociation of PKD were inhibited either by preventing the plasma membrane translocation of PKCepsilon, through abolition of its interaction with receptor for activated C kinase (RACK), or by suppressing the expression of PKCepsilon via specific small interfering RNAs. Thus, this study demonstrates that the plasma membrane translocation of PKD, in response to GPCR stimulation, is necessary for the PKCepsilon-mediated phosphorylation of the activation loop of PKD and that this event requires the translocation of both kinases to the plasma membrane. Based on these and previous results, we propose a model of GPCR-mediated PKD regulation that integrates its changes in distribution, catalytic activity and multi-site phosphorylation.
J. Biol. Chem, 10.1074/jbc.M403265200
Submitted on March 24, 2004
Revised on June 8, 2004
Accepted on June 8, 2004
G protein-coupled receptor-mediated phosphorylation of the activation loop of protein kinase D: Dependence on plasma membrane translocation and protein kinase c epsilon
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Jacamo, J. Sinnett-Smith, O. Rey, R. T. Waldron, and E. Rozengurt Sequential Protein Kinase C (PKC)-dependent and PKC-independent Protein Kinase D Catalytic Activation via Gq-coupled Receptors: DIFFERENTIAL REGULATION OF ACTIVATION LOOP SER744 AND SER748 PHOSPHORYLATION J. Biol. Chem., May 9, 2008; 283(19): 12877 - 12887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. Poole, S. Amadesi, E. Rozengurt, M. Thacker, N. W. Bunnett, and J. B. Furness Stimulation of the neurokinin 3 receptor activates protein kinase C{varepsilon} and protein kinase D in enteric neurons Am J Physiol Gastrointest Liver Physiol, May 1, 2008; 294(5): G1245 - G1256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. McEneaney, B. J. Harvey, and W. Thomas Aldosterone Regulates Rapid Trafficking of Epithelial Sodium Channel Subunits in Renal Cortical Collecting Duct Cells via Protein Kinase D Activation Mol. Endocrinol., April 1, 2008; 22(4): 881 - 892. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Avkiran, A. J. Rowland, F. Cuello, and R. S. Haworth Protein Kinase D in the Cardiovascular System: Emerging Roles in Health and Disease Circ. Res., February 1, 2008; 102(2): 157 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. R. Murphy, H. J. Legere III, and H. R. Katz Activation of Protein Kinase D1 in Mast Cells in Response to Innate, Adaptive, and Growth Factor Signals J. Immunol., December 1, 2007; 179(11): 7876 - 7882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Romero, B. L. Welsh, E. P. Gomez-Sanchez, L. L. Yanes, S. Rilli, and C. E. Gomez-Sanchez Angiotensin II-Mediated Protein Kinase D Activation Stimulates Aldosterone and Cortisol Secretion in H295R Human Adrenocortical Cells Endocrinology, December 1, 2006; 147(12): 6046 - 6055. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Amadesi, G. S. Cottrell, L. Divino, K. Chapman, E. F. Grady, F. Bautista, R. Karanjia, C. Barajas-Lopez, S. Vanner, N. Vergnolle, et al. Protease-activated receptor 2 sensitizes TRPV1 by protein kinase C{varepsilon}- and A-dependent mechanisms in rats and mice J. Physiol., September 1, 2006; 575(2): 555 - 571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Rey, R. Papazyan, R. T. Waldron, S. H. Young, J. Lippincott-Schwartz, R. Jacamo, and E. Rozengurt The Nuclear Import of Protein Kinase D3 Requires Its Catalytic Activity J. Biol. Chem., February 24, 2006; 281(8): 5149 - 5157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Rey, S. H. Young, J. Yuan, L. Slice, and E. Rozengurt Amino Acid-stimulated Ca2+ Oscillations Produced by the Ca2+-sensing Receptor Are Mediated by a Phospholipase C/Inositol 1,4,5-Trisphosphate-independent Pathway That Requires G12, Rho, Filamin-A, and the Actin Cytoskeleton J. Biol. Chem., June 17, 2005; 280(24): 22875 - 22882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. D. Anel and V. Malhotra PKC{eta} is required for {beta}1{gamma}2/{beta}3{gamma}2- and PKD-mediated transport to the cell surface and the organization of the Golgi apparatus J. Cell Biol., April 11, 2005; 169(1): 83 - 91. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Rozengurt, O. Rey, and R. T. Waldron Protein Kinase D Signaling J. Biol. Chem., April 8, 2005; 280(14): 13205 - 13208. [Full Text] [PDF]
|
|
|
|
|
|
C. D. Wood, U. Marklund, and D. A. Cantrell Dual Phospholipase C/Diacylglycerol Requirement for Protein Kinase D1 Activation in Lymphocytes J. Biol. Chem., February 18, 2005; 280(7): 6245 - 6251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. B. Vega, B. C. Harrison, E. Meadows, C. R. Roberts, P. J. Papst, E. N. Olson, and T. A. McKinsey Protein Kinases C and D Mediate Agonist-Dependent Cardiac Hypertrophy through Nuclear Export of Histone Deacetylase 5 Mol. Cell. Biol., October 1, 2004; 24(19): 8374 - 8385. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
