| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print April 24, 2004
Department of Biochemistry, University of Oxford, Oxford OX1 3QU
Corresponding Author: nicholas.kent{at}bioch.ox.ac.uk
Cbf1p is a basic-helix-loop-helix-zipper protein of Saccharomyces cerevisiae required for the function of centromeres and MET gene promoters where it binds DNA via the consensus core motif CACRTG (R=A or G). At MET genes Cbf1p appears to function in both activator recruitment and chromatin-remodeling. Cbf1p has been implicated in the regulation of other genes and CACRTG motifs are common in potential gene-regulatory DNA. A recent genome-wide location analysis showed that the majority of intergenic CACGTG palindromes are bound by Cbf1p. Here we tested whether all potential Cbf1p-binding motifs in the yeast genome are likely to be bound by Cbf1p using chromatin immunoprecipitation. We also tested which of the motifs are actually functional by assaying for Cbf1p-dependent chromatin remodeling. We show that Cbf1p binding and activity is restricted to palindromic CACGTG motifs in promoter-proximal regions. Cbf1p does not function through CACGTG motifs which occur in promoter-distal locations within coding regions, nor where CACATG motifs occur alone, except at centromeres. Cbf1p can be made to function at promoter-distal CACGTG motifs by over-expression, suggesting that the concentration of Cbf1p is normally limiting for binding and is biased to gene-regulatory DNA by interactions with other factors. We conclude that Cbf1p is required for normal nucleosome positioning wherever the CACGTG motif occurs in gene-regulatory DNA. Cbf1p has been shown to interact with the chromatin-remodeling ATPase Isw1p. Here we show that recruitment of Isw1p by Cbf1p is likely to be general, but that Isw1p is only partially required for Cbf1p-dependent chromatin structures.
J. Biol. Chem, 10.1074/jbc.M403818200
Submitted on April 6, 2004
Revised on April 23, 2004
Accepted on April 23, 2004
Cbf1p is required for chromatin remodeling at promoter-proximal CACGTG motifs in yeast
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J.-N. Park, M. J. Sohn, D.-B. Oh, O. Kwon, S. K. Rhee, C.-G. Hur, S. Y. Lee, G. Gellissen, and H. A. Kang Identification of the Cadmium-Inducible Hansenula polymorpha SEO1 Gene Promoter by Transcriptome Analysis and Its Application to Whole-Cell Heavy-Metal Detection Systems Appl. Envir. Microbiol., October 1, 2007; 73(19): 5990 - 6000. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Chen and J. M. Lopes Multiple Basic Helix-Loop-Helix Proteins Regulate Expression of the ENO1 Gene of Saccharomyces cerevisiae Eukaryot. Cell, May 1, 2007; 6(5): 786 - 796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. B. Murray, M. Beckmann, and H. Kitano Regulation of yeast oscillatory dynamics PNAS, February 13, 2007; 104(7): 2241 - 2246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Maerkl and S. R. Quake A Systems Approach to Measuring the Binding Energy Landscapes of Transcription Factors Science, January 12, 2007; 315(5809): 233 - 237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-C. Lai, A. L. Kosorukoff, P. V. Burke, and K. E. Kwast Metabolic-State-Dependent Remodeling of the Transcriptome in Response to Anoxia and Subsequent Reoxygenation in Saccharomyces cerevisiae. Eukaryot. Cell, September 1, 2006; 5(9): 1468 - 1489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Menant, P. Baudouin-Cornu, C. Peyraud, M. Tyers, and D. Thomas Determinants of the Ubiquitin-mediated Degradation of the Met4 Transcription Factor J. Biol. Chem., April 28, 2006; 281(17): 11744 - 11754. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
