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Papers In Press, published online ahead of print July 13, 2004
School of Biological Sciences, Seoul National University, Seoul, Seoul 151-742
Corresponding Author: yjseok{at}plaza.snu.ac.kr
Because the phosphoenolpyruvate:sugar phosphotransferase system (PTS) plays multiple regulatory roles in addition to the phosphorylation-coupled transport of many sugars in bacteria, synthesis of its protein components is regulated in a highly sophisticated way. Thus far the cAMP·CRP complex and Mlc are known to be the major regulators of ptsHIcrr and ptsG expression in response to the availability of carbon sources. In this report, we performed ligand-fishing experiments using the promoters of ptsHIcrr and ptsG as baits to find out new factors involved in the transcriptional regulation of PTS in Escherichia coli, and found that the anaerobic regulator ArcA specifically binds to the promoters. Deletion of the arcA gene caused about two-fold increase in the ptsG expression and overexpression of ArcA significantly decreased glucose consumption. In vitro transcription assays showed that phospho-ArcA (ArcA-P) represses ptsG P1 transcription. DNase I footprinting experiment revealed that ArcA-P binds to three sites upstream of the ptsG P1 promoter, two of which overlap the CRP binding sites, and the ArcA-P binding decreases the CRP binding which is essential for the ptsG P1 transcription. These results suggest that the response regulator ArcA regulates expression of enzyme IICBGlc mediating the first step of glucose metabolism in response to the redox conditions of growth in E. coli.
J. Biol. Chem, 10.1074/jbc.M406667200
Submitted on June 15, 2004
Revised on July 7, 2004
Accepted on July 13, 2004
Expression of ptsG encoding the major glucose transporter is regulated by ArcA in Escherichia coli
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