| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print September 15, 2004
Division of Biology, California Institute of Technology, Pasadena, CA 91125
Corresponding Author: dunphy{at}cco.caltech.edu
Claspin is necessary for the ATR-dependent activation of Chk1 in Xenopus egg extracts containing incompletely replicated DNA. ATR possesses a regulatory partner called ATRIP. We have studied the respective roles of ATR-ATRIP and Claspin in the activation of Chk1. ATR-ATRIP binds well to various DNA templates in Xenopus egg extracts. ATR-ATRIP bound to a single-stranded DNA template is weakly active. By contrast, the ATR-ATRIP complex on a DNA template containing both single-stranded and double-stranded regions displays a large increase in kinase activity. This observation suggests that ATR-ATRIP normally undergoes activation upon association with specific nucleic acid structures at DNA replication forks. Without Claspin, activated ATR-ATRIP phosphorylates Chk1 weakly in a cell-free reaction. Addition of Claspin to this reaction strongly stimulates the phosphorylation of Chk1 by ATR-ATRIP. Claspin also induces significant autophosphorylation of Chk1 in the absence of ATR-ATRIP. Taken together, these results indicate that the checkpoint-dependent phosphorylation of Chk1 is a multi-step process involving activation of the ATR-ATRIP complex at replication forks and presentation of Chk1 to this complex by Claspin.
J. Biol. Chem, 10.1074/jbc.M408353200
Submitted on July 23, 2004
Revised on September 13, 2004
Accepted on September 15, 2004
Claspin and the activated form of ATR-ATRIP collaborate in the activation of Chk1
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Lee, A. Kumagai, and W. G. Dunphy The Rad9-Hus1-Rad1 Checkpoint Clamp Regulates Interaction of TopBP1 with ATR J. Biol. Chem., September 21, 2007; 282(38): 28036 - 28044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Errico, V. Costanzo, and T. Hunt Tipin is required for stalled replication forks to resume DNA replication after removal of aphidicolin in Xenopus egg extracts PNAS, September 18, 2007; 104(38): 14929 - 14934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Verlinden, I. Vanden Bempt, G. Eelen, M. Drijkoningen, I. Verlinden, K. Marchal, C. De Wolf-Peeters, M.-R. Christiaens, L. Michiels, R. Bouillon, et al. The E2F-Regulated Gene Chk1 Is Highly Expressed in Triple-Negative Estrogen Receptor /Progesterone Receptor /HER-2 Breast Carcinomas Cancer Res., July 15, 2007; 67(14): 6574 - 6581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Y. Yoo, A. Kumagai, A. Shevchenko, A. Shevchenko, and W. G. Dunphy Ataxia-telangiectasia Mutated (ATM)-dependent Activation of ATR Occurs through Phosphorylation of TopBP1 by ATM J. Biol. Chem., June 15, 2007; 282(24): 17501 - 17506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zou Single- and double-stranded DNA: building a trigger of ATR-mediated DNA damage response Genes & Dev., April 15, 2007; 21(8): 879 - 885. [Full Text] [PDF]
|
|
|
|
|
|
H. Ishii, K. Mimori, H. Inoue, T. Inageta, K. Ishikawa, S. Semba, T. Druck, F. Trapasso, K. Tani, A. Vecchione, et al. Fhit Modulates the DNA Damage Checkpoint Response Cancer Res., December 1, 2006; 66(23): 11287 - 11292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. Gastwirt, D. A. Slavin, C. W. McAndrew, and D. J. Donoghue Spy1 Expression Prevents Normal Cellular Responses to DNA Damage: INHIBITION OF APOPTOSIS AND CHECKPOINT ACTIVATION J. Biol. Chem., November 17, 2006; 281(46): 35425 - 35435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. S. Chini and J. Chen Repeated Phosphopeptide Motifs in Human Claspin Are Phosphorylated by Chk1 and Mediate Claspin Function J. Biol. Chem., November 3, 2006; 281(44): 33276 - 33282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Chang, P. J. Lupardus, and K. A. Cimprich Monoubiquitination of Proliferating Cell Nuclear Antigen Induced by Stalled Replication Requires Uncoupling of DNA Polymerase and Mini-chromosome Maintenance Helicase Activities J. Biol. Chem., October 27, 2006; 281(43): 32081 - 32088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Colton, X. S. Xu, Y. A. Wang, and G. Wang The Involvement of Ataxia-telangiectasia Mutated Protein Activation in Nucleotide Excision Repair-facilitated Cell Survival with Cisplatin Treatment J. Biol. Chem., September 15, 2006; 281(37): 27117 - 27125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Rodriguez-Bravo, S. Guaita-Esteruelas, R. Florensa, O. Bachs, and N. Agell Chk1- and Claspin-Dependent but ATR/ATM- and Rad17-Independent DNA Replication Checkpoint Response in HeLa Cells. Cancer Res., September 1, 2006; 66(17): 8672 - 8679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hashimoto, T. Tsujimura, A. Sugino, and H. Takisawa The phosphorylated C-terminal domain of Xenopus Cut5 directly mediates ATR-dependent activation of Chk1. Genes Cells, September 1, 2006; 11(9): 993 - 1007. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Liu, S. Bekker-Jensen, N. Mailand, C. Lukas, J. Bartek, and J. Lukas Claspin Operates Downstream of TopBP1 To Direct ATR Signaling towards Chk1 Activation. Mol. Cell. Biol., August 1, 2006; 26(16): 6056 - 6064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Y. Yoo, S.-Y. Jeong, and W. G. Dunphy Site-specific phosphorylation of a checkpoint mediator protein controls its responses to different DNA structures Genes & Dev., April 1, 2006; 20(7): 772 - 783. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Lupardus and K. A. Cimprich Phosphorylation of Xenopus Rad1 and Hus1 Defines a Readout for ATR Activation That Is Independent of Claspin and the Rad9 Carboxy Terminus Mol. Biol. Cell, April 1, 2006; 17(4): 1559 - 1569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Namiki and L. Zou ATRIP associates with replication protein A-coated ssDNA through multiple interactions PNAS, January 17, 2006; 103(3): 580 - 585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Itakura, I. Sawada, and A. Matsuura Dimerization of the ATRIP Protein through the Coiled-Coil Motif and Its Implication to the Maintenance of Stalled Replication Forks Mol. Biol. Cell, December 1, 2005; 16(12): 5551 - 5562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-M. Kim, A. Kumagai, J. Lee, and W. G. Dunphy Phosphorylation of Chk1 by ATM- and Rad3-related (ATR) in Xenopus Egg Extracts Requires Binding of ATRIP to ATR but Not the Stable DNA-binding or Coiled-coil Domains of ATRIP J. Biol. Chem., November 18, 2005; 280(46): 38355 - 38364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, S. Bao, R. Furumai, K. S. Kucera, A. Ali, N. M. Dean, and X.-F. Wang Protein Phosphatase 5 Is Required for ATR-Mediated Checkpoint Activation Mol. Cell. Biol., November 15, 2005; 25(22): 9910 - 9919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lee, D. A. Gold, A. Shevchenko, A. Shevchenko, and W. G. Dunphy Roles of Replication Fork-interacting and Chk1-activating Domains from Claspin in a DNA Replication Checkpoint Response Mol. Biol. Cell, November 1, 2005; 16(11): 5269 - 5282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. L. Clarke, L. N. Bennett, and P. R. Clarke Cleavage of Claspin by Caspase-7 during Apoptosis Inhibits the Chk1 Pathway J. Biol. Chem., October 21, 2005; 280(42): 35337 - 35345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cortez Unwind and slow down: checkpoint activation by helicase and polymerase uncoupling Genes & Dev., May 1, 2005; 19(9): 1007 - 1012. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
