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Papers In Press, published online ahead of print October 25, 2004
School of Biology, Georgia Institute of Technology, Atlanta, GA 30332-0230
Corresponding Author: nael.mccarty{at}biology.gatech.edu
The magnitudes and distributions of subconductance states were studied in chloride channels formed by the wild-type cystic fibrosis transmembrane conductance regulator (CFTR) and in CFTRs bearing amino acid substitutions in transmembrane segment six (TM6). Within an open burst it was possible to distinguish three, distinct conductance states referred to as the full conductance (f), subconductance 1 (s1) and subconductance 2 (s2) states. Amino acid substitutions in TM6 altered the duration and probability of occurrence of these subconductance states, but did not greatly alter their relative amplitudes. Results from real-time measurements indicated that covalent modification of single R334C-CFTR channels by MTSET+ ([2-(trimethylammonium)ethyl] methanethiosulfonate) resulted in the simultaneous modification of all three conductance levels in what appeared to be a single step, without changing the proportion of time spent in each state. This behavior suggests that at least a portion of the conduction path is common to all three conducting states. The time course for the modification of R334C-CFTR, measured in outside-out macropatches using a rapid perfusion system, was also consistent with a single modification step as if each pore contained only a single copy of the cysteine at position 334. These results are consistent with a model for the CFTR conduction pathway in which a single anion-conducting pore is formed by a single CFTR polypeptide.
J. Biol. Chem, 10.1074/jbc.M409626200
Submitted on August 23, 2004
Revised on October 18, 2004
Accepted on October 25, 2004
Determination of the functional unit of the CFTR chloride channel: One polypeptide forms one pore
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