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Papers In Press, published online ahead of print February 8, 2005
Pathology, Yale University, New Haven, CT 06510
Corresponding Author: wang.min{at}yale.edu
Apoptosis signal-regulating kinase 1 (ASK1)-JNK/p38 signaling pathway is pivotal component in cell apoptosis and can be activated by a variety of death stimuli including tumor necrosis factor alpha (TNF) and oxidative stress (ROS). However, the mechanism for ASK1 activation is not fully understood. We have recently identified AIP1 as novel signal transducer in TNF-induced ASK1 activation by facilitating dissociation of ASK1 from its inhibitor 14-3-3. In the present study, we employed yeast two-hybrid system using the N-terminal domain of AIP1 as bait and identified HIPK1 as an AIP1-associated protein. Interestingly, we showed that TNF induced HIPK1 de-sumoylation concomitant with a translocation from nucleus to cytoplasm at 15 minutes followed by a return to nucleus by 60 min. The kinetics of HIPK1 translocation correlates with those of stress-induced ASK1-JNK/P38 activation. A specific JNK inhibitor blocked the reverse but the initial translocation of HIPK1, suggesting that the initial translocation is an upstream event of ASK1-JNK/p38 signaling and JNK activation regulates the reverse translocation as a feedback mechanism. Consistently, expression of HIPK1 increased, whereas expression of a kinase-inactive form (HIPK1-D315N) or SiRNA of HIPK1 decreased stress-induced ASK1-JNK/P38 activation without effects on IKK-NF-kB signaling. Moreover, a sumoylation-defective mutant of HIPK1 (KR5) localizes to the cytoplasm and is constitutively active in ASK1-JNK/P38 activation. Furthermore, HIPK1-KR5 induces dissociation of ASK1 from its inhibitors 14-3-3 and thioredoxin, and synergizes with AIP1 to induce ASK1 activation. Our study suggests that TNF-induced desumoylation and cytoplasmic translocation of HIPK1 are critical in TNF-induced ASK1-JNK/p38 activation.
J. Biol. Chem, 10.1074/jbc.M414262200
Submitted on December 20, 2004
Revised on February 1, 2005
Accepted on February 8, 2005
TNF-induced desumoylation and cytoplasmic translocation of HIPK1 are critical for ASK1-JNK/p38 activation
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