| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print April 29, 2005
J. Biol. Chem, 10.1074/jbc.M502475200
Submitted on March 7, 2005
Revised on April 26, 2005
Accepted on April 28, 2005
School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853-2501
Corresponding Author: www2{at}cornell.edu
Global analysis of fluorescence and associated anisotropy decays of intrinsic tissue fluorescence offers a sensitive and non-invasive probe of the metabolically critical free/enzyme-bound states of intracellular 
-nicotinamide adenine dinucleotide (NADH) in neural tissue. Using this technique, we demonstrate that the response of NADH to the metabolic transition from normoxia to hypoxia is more complex than a simple increase in NADH concentration. The concentration of free NADH, and that of an enzyme bound form with a relatively low lifetime, increases preferentially over that of other enzyme bound NADH species. Concomitantly, the intracellular viscosity is reduced, likely due to the osmotic swelling of mitochondria. These conformation and environmental changes effectively decrease the tissue fluorescence average lifetime, causing the usual total fluorescence increase measurements to significantly underestimate the calculated concentration increase. This new discrimination of changes in NADH concentration, conformation and environment provides the foundation for quantitative functional imaging of neural energy metabolism.
This article has been cited by other articles:
|
|
 |
|
  M. C. Skala, K. M. Riching, A. Gendron-Fitzpatrick, J. Eickhoff, K. W. Eliceiri, J. G. White, and N. Ramanujam In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia PNAS, December 4, 2007; 104(49): 19494 - 19499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Davey, R. P. Walvick, Y. Liu, A. A. Heikal, and E. D. Sheets Membrane Order and Molecular Dynamics Associated with IgE Receptor Cross-Linking in Mast Cells Biophys. J., January 1, 2007; 92(1): 343 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Yuan, E. Rhoades, X. W. Lou, and L. A. Archer Spontaneous sharp bending of DNA: role of melting bubbles Nucleic Acids Res., September 11, 2006; 34(16): 4554 - 4560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Vlassenko, M. M. Rundle, M. E. Raichle, and M. A. Mintun Regulation of blood flow in activated human brain by cytosolic NADH/NAD+ ratio PNAS, February 7, 2006; 103(6): 1964 - 1969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Yuan, E. Rhoades, D. M. Heuer, and L. A. Archer Mismatch-Induced DNA Unbending upon Duplex Opening Biophys. J., October 1, 2005; 89(4): 2564 - 2573. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
