Dissection of a circumscribed recombination hot spot in HIV-1 after a single infectious cycle

doi:10.1074/jbc.M505457200

HOME

QUICK SEARCH:

	Author:		Keyword(s):
Year:		Vol:		Page:

A more recent version of this article appeared on February 3, 2006

This Article

	Full Text (Accepted Manuscript)
	Supplemental Data
	All Versions of this Article: 281/5/2711 most recent M505457200v1
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Email this article to a friend
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Request Permissions

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Galetto, R.
	Articles by Negroni, M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Galetto, R.
	Articles by Negroni, M.

Social Bookmarking

	What's this?

Papers In Press, published online ahead of print November 15, 2005
J. Biol. Chem, 10.1074/jbc.M505457200
Submitted on May 18, 2005
Revised on November 14, 2005
Accepted on November 14, 2005

Dissection of a circumscribed recombination hot spot in HIV-1 after a single infectious cycle

Román Galetto, Véronique Giacomoni, Michel Véron, and Matteo Negroni

Chemistry and structural biology, Institut Pasteur, Paris 75724

Corresponding Author: matteo{at}pasteur.fr

Recombination is a major source of genetic heterogeneity in the human immunodeficiency virus type 1 (HIV-1) population. The main mechanism responsible for the generation of recombinant viruses is a process of copy choice between the two copies of genomic RNA during reverse transcription. We previously identified, after a single cycle of infection of cells in culture, a recombination hot spot within the gp120 gene, corresponding to the top portion of a RNA hairpin. Here, we determine that the hot region is circumscribed to 18 nucleotides located in the descending strand of the stem, following the sense of reverse transcription. Three factors appeared to be important, albeit at different extents, for the high rate of recombination observed in this region. The position of the hot sequence in the context of the RNA structure appears crucial, since changing its location within this structure triggered differences in recombination up to 20 fold. Another pivotal factor is the presence of a perfectly identical sequence between donor and acceptor RNA in the region of transfer, since single or double nucleotide differences in the hot spot were sufficient to almost completely abolish recombination in the region. Last, the primary structure of the hot region also influenced recombination, although with effects only in the two- to three- fold range. Altogether, these results provide the first molecular dissection of a hot spot in infected cells, and indicate that several factors contribute to the generation of a site of preferential copy choice in infected cells.

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

A. Galli, A. Lai, S. Corvasce, F. Saladini, C. Riva, L. Deho, I. Caramma, M. Franzetti, L. Romano, M. Galli, et al.
Recombination analysis and structure prediction show correlation between breakpoint clusters and RNA hairpins in the pol gene of human immunodeficiency virus type 1 unique recombinant forms
J. Gen. Virol., December 1, 2008; 89(12): 3119 - 3125.
[Abstract] [Full Text] [PDF]

Q. Wang, I. Barr, F. Guo, and C. Lee
Evidence of a novel RNA secondary structurein the coding region of HIV-1 pol gene
RNA, December 1, 2008; 14(12): 2478 - 2488.
[Abstract] [Full Text] [PDF]

T. Nora, C. Charpentier, O. Tenaillon, C. Hoede, F. Clavel, and A. J. Hance
Contribution of Recombination to the Evolution of Human Immunodeficiency Viruses Expressing Resistance to Antiretroviral Treatment
J. Virol., July 15, 2007; 81(14): 7620 - 7628.
[Abstract] [Full Text] [PDF]

T. Wu, S. L. Heilman-Miller, and J. G. Levin
Effects of nucleic acid local structure and magnesium ions on minus-strand transfer mediated by the nucleic acid chaperone activity of HIV-1 nucleocapsid protein
Nucleic Acids Res., June 9, 2007; 35(12): 3974 - 3987.
[Abstract] [Full Text] [PDF]

L. Gao, M. Balakrishnan, B. P. Roques, and R. A. Bambara
Insights into the Multiple Roles of Pausing in HIV-1 Reverse Transcriptase-promoted Strand Transfers
J. Biol. Chem., March 2, 2007; 282(9): 6222 - 6231.
[Abstract] [Full Text] [PDF]

H. A. Baird, R. Galetto, Y. Gao, E. Simon-Loriere, M. Abreha, J. Archer, J. Fan, D. L. Robertson, E. J. Arts, and M. Negroni
Sequence determinants of breakpoint location during HIV-1 intersubtype recombination
Nucleic Acids Res., October 6, 2006; (2006) gkl669v3.
[Abstract] [Full Text] [PDF]