Distinct regulatory elements mediate similar expression patterns in Caenorhabditis elegans' excretory cell

doi:10.1074/jbc.M505701200

HOME

QUICK SEARCH:

	Author:		Keyword(s):
Year:		Vol:		Page:

A more recent version of this article appeared on November 18, 2005

This Article

	Full Text (Accepted Manuscript)
	Supplemental Data
	All Versions of this Article: 280/46/38787 most recent M505701200v1
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Email this article to a friend
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Request Permissions

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Zhao, Z.
	Articles by Baillie, D. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Zhao, Z.
	Articles by Baillie, D. L.

Social Bookmarking

	What's this?

Papers In Press, published online ahead of print September 13, 2005
J. Biol. Chem, 10.1074/jbc.M505701200
Submitted on May 25, 2005
Accepted on September 13, 2005

Distinct regulatory elements mediate similar expression patterns in Caenorhabditis elegans' excretory cell

Zhongying Zhao, Li Fang, Nansheng Chen, Robert C. Johnsen, Lincoln Stein, and David L. Baillie

Department of Genome Sciences, University of Washington, Seattle, WA 98195-7730

Corresponding Author: zhao{at}gs.washington.edu

Identification of cis-regulatory elements and their binding proteins constitutes an important part of understanding gene function and regulation. It is well accepted that co-expressed genes tend to share transcriptional elements. However, recent findings indicate that co-expression data show poor correlation with co-regulation data even in unicellular yeast. This motivates us to experimentally explore whether it is possible that co-expressed genes are subject to differential regulatory control using C. elegans’ excretory cell as an example. Excretory cell is a functional equivalent of human kidney. Transcriptional regulation of gene expression in the cell is largely unknown. We isolated a 10 bp excretory cell specific cis-element, Ex-1 from a pgp-12 promoter. The significance of the element has been demonstrated by its capacity of converting an intestine specific promoter into an excretory cell-specific one. We also isolated a cDNA encoding an Ex-1 binding transcription factor, DCP-66 using a yeast one-hybrid screen. Role of the factor in regulation of pgp-12 expression has been demonstrated both in vitro and in vivo. Search for occurrence of Ex-1 reveals that only a small portion of excretory cell-specific promoters contain Ex-1. Two other distinct cis-elements isolated from two different promoters can also dictate the excretory cell-specific expression but are independent of regulation by DCP-66. The results indicate that distinct regulatory elements are able to mediate the similar expression patterns.

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

A. K. Mah, K. R. Armstrong, D. S. Chew, J. S. Chu, D. K. Tu, R. C. Johnsen, N. Chen, H. M. Chamberlin, and D. L. Baillie
Transcriptional Regulation of AQP-8, a Caenorhabditis elegans Aquaporin Exclusively Expressed in the Excretory System, by the POU Homeobox Transcription Factor CEH-6
J. Biol. Chem., September 21, 2007; 282(38): 28074 - 28086.
[Abstract] [Full Text] [PDF]

G. Zhao, L. A. Schriefer, and G. D. Stormo
Identification of muscle-specific regulatory modules in Caenorhabditis elegans
Genome Res., March 1, 2007; 17(3): 348 - 357.
[Abstract] [Full Text] [PDF]

Z. Zhao, J. H. Thomas, N. Chen, J. A. Sheps, and D. L. Baillie
Comparative Genomics and Adaptive Selection of the ATP-Binding-Cassette Gene Family in Caenorhabditis Species
Genetics, March 1, 2007; 175(3): 1407 - 1418.
[Abstract] [Full Text] [PDF]