Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on October 21, 2005
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
280/42/35209    most recent
M505815200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, K.
Right arrow Articles by Kim, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, K.
Right arrow Articles by Kim, N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print August 18, 2005
J. Biol. Chem, 10.1074/jbc.M505815200
Submitted on May 27, 2005
Revised on August 18, 2005
Accepted on August 18, 2005

NFATc1 induces OSCAR gene expression during TRANCE-mediated osteoclastogenesis

Kabsun Kim, Jung Ha Kim, Junwon Lee, Hye-Mi Jin, Seoung-Hoon Lee, David E. Fisher, Hyun Kook, Kyung Keun Kim, Yongwon Choi, and Nacksung Kim

Medical Research Center for Gene Regulation, Chonnam National University Medical School, Gwangju 501-746

Corresponding Author: nacksung{at}jnu.ac.kr or nacksungkim@hotmail.com

Osteoclast differentiation from hematopoietic precursors is controlled by the TNF family member TRANCE via induction of various transcription factors, including nuclear factor of activated T cells (NFAT) c1. During osteoclast differentiation, NFATc1 is further activated via calcium signaling when costimulatory receptors expressed on osteoclast precursors, such as osteoclast-associated receptor (OSCAR), are stimulated. Here we show that NFATc1 expression precedes that of OSCAR during TRANCE-mediated osteoclastogenesis, and that inhibition of NFATc1 by cyclosporin A abolishes TRANCE-induced OSCAR expression and subsequent osteoclast differentiation. Moreover, we show that the 1.0-Kb promoter region of the OSCAR gene contains three potential NFATc1-binding sites. Induction of an OSCAR promoter-luciferase reporter is significantly increased when transiently transfected into 293T cells in combination with NFATc1 expression plasmid. Deletion and site-directed mutant constructs confirmed that NFATc1-binding sites are both functional and NFATc1-specific. Furthermore, NFATc1 synergistically activates an OSCAR reporter construct together with microphthalmia transcription factor (Mitf) and PU.1, transcription factors previously shown to be critical for osteoclast differentiation. In addition, a plasmid expressing constitutively active MKK6 enhances the transactivation activity of NFATc1/Mitf/PU.1 on the OSCAR promoter. Taken together, our results indicate that NFATc1 is an important transcription factor in the induction of OSCAR during osteoclastogenesis. Elucidation of NFATc1 as a transcription factor for OSCAR expression implies the presence of a positive feedback circuit of TRANCE-induced activation of NFATc1, involving NFATc1-mediated OSCAR expression, and its subsequent activation of NFATc1, necessary for efficient differentiation of osteoclasts.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
E.-J. Chang, J. Ha, H. Huang, H. J. Kim, J. H. Woo, Y. Lee, Z. H. Lee, J. H. Kim, and H.-H. Kim
The JNK-dependent CaMK pathway restrains the reversion of committed cells during osteoclast differentiation
J. Cell Sci., August 1, 2008; 121(15): 2555 - 2564.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
K. Kim, S.-H. Lee, J. Ha Kim, Y. Choi, and N. Kim
NFATc1 Induces Osteoclast Fusion Via Up-Regulation of Atp6v0d2 and the Dendritic Cell-Specific Transmembrane Protein (DC-STAMP)
Mol. Endocrinol., January 1, 2008; 22(1): 176 - 185.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. M. Sharma, A. Bronisz, R. Hu, K. Patel, K. C. Mansky, S. Sif, and M. C. Ostrowski
MITF and PU.1 Recruit p38 MAPK and NFATc1 to Target Genes during Osteoclast Differentiation
J. Biol. Chem., May 25, 2007; 282(21): 15921 - 15929.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Kim, J. Lee, J. H. Kim, H. M. Jin, B. Zhou, S. Y. Lee, and N. Kim
Protein Inhibitor of Activated STAT 3 Modulates Osteoclastogenesis by Down-Regulation of NFATc1 and Osteoclast-Associated Receptor
J. Immunol., May 1, 2007; 178(9): 5588 - 5594.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. Kim, J. H. Kim, J. Lee, H. M. Jin, H. Kook, K. K. Kim, S. Y. Lee, and N. Kim
MafB negatively regulates RANKL-mediated osteoclast differentiation
Blood, April 15, 2007; 109(8): 3253 - 3259.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. Lee, K. Kim, J. H. Kim, H. M. Jin, H. K. Choi, S.-H. Lee, H. Kook, K. K. Kim, Y. Yokota, S. Y. Lee, et al.
Id helix-loop-helix proteins negatively regulate TRANCE-mediated osteoclast differentiation
Blood, April 1, 2006; 107(7): 2686 - 2693.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. S. Kim, C. J. Day, C. I. Selinger, C. L. Magno, S. R. J. Stephens, and N. A. Morrison
MCP-1-induced Human Osteoclast-like Cells Are Tartrate-resistant Acid Phosphatase, NFATc1, and Calcitonin Receptor-positive but Require Receptor Activator of NF{kappa}B Ligand for Bone Resorption
J. Biol. Chem., January 13, 2006; 281(2): 1274 - 1285.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement