Stimulation of DNA strand exchange by the human TBPIP/HOP2-MND1 complex

doi:10.1074/jbc.M506506200

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Papers In Press, published online ahead of print January 9, 2006
J. Biol. Chem, 10.1074/jbc.M506506200
Submitted on June 15, 2005
Revised on December 20, 2005
Accepted on January 9, 2006

Stimulation of DNA strand exchange by the human TBPIP/HOP2-MND1 complex

Rima Enomoto, Takashi Kinebuchi, Makoto Sato, Hideshi Yagi, Hitoshi Kurumizaka, and Shigeyuki Yokoyama

Graduate School of Science and Engineering, Waseda University, Tokyo 169-8555

Corresponding Author: kurumizaka{at}waseda.jp

In Saccharomyces cerevisiae, the Hop2 protein forms a complex with the Mnd1 protein, and is required for the alignment of homologous chromosomes during meiosis, probably through extensive homology matching between them. The Rad51 and Dmc1 proteins, the eukaryotic RecA orthologs, promote strand exchange, and may function in the extensive matching of homology within paired DNA molecules. In the present study, we purified the human TBPIP/Hop2-Mnd1 complex, and found that it significantly stimulates the Dmc1- and Rad51-mediated strand exchange. The human Hop2-Mnd1 complex preferentially binds to a three-stranded DNA branch, which mimics the strand-exchange intermediate. These findings are consistent with genetic data, which showed that the Hop2 and Mnd1 proteins are required for homology matching between homologous chromosomes. Therefore, the human TBPIP/Hop2-Mnd1 complex may ensure proper pairing between homologous chromosomes through its stimulation of strand exchange during meiosis.

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				P. Chi, J. San Filippo, M. G. Sehorn, G. V. Petukhova, and P. Sung Bipartite stimulatory action of the Hop2-Mnd1 complex on the Rad51 recombinase Genes & Dev., July 15, 2007; 21(14): 1747 - 1757. [Abstract] [Full Text] [PDF]

				R. J. Pezza, O. N. Voloshin, F. Vanevski, and R. D. Camerini-Otero Hop2/Mnd1 acts on two critical steps in Dmc1-promoted homologous pairing Genes & Dev., July 15, 2007; 21(14): 1758 - 1766. [Abstract] [Full Text] [PDF]

				M. Ploquin, G. V. Petukhova, D. Morneau, U. Dery, A. Bransi, A. Stasiak, R. D. Camerini-Otero, and J.-Y. Masson Stimulation of fission yeast and mouse Hop2-Mnd1 of the Dmc1 and Rad51 recombinases Nucleic Acids Res., April 10, 2007; (2007) gkm174v1. [Abstract] [Full Text] [PDF]