JBC Oz Biosciences

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on November 4, 2005
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
280/44/36545    most recent
M506670200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lingwood, D.
Right arrow Articles by Ballantyne, J. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lingwood, D.
Right arrow Articles by Ballantyne, J. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print August 11, 2005
J. Biol. Chem, 10.1074/jbc.M506670200
Submitted on June 20, 2005
Revised on July 13, 2005
Accepted on August 11, 2005

Regulation of fish gill Na+-K+-ATPase by selective sulfatide-enriched raft partitioning during seawater adaptation

D. Lingwood, G. Harauz, and J. S. Ballantyne

Department of Integrative Biology, University of Guelph, Guelph, ON N1G 2W1

Corresponding Author: dlingwoo{at}uoguelph.ca

Na+-K+-ATPase is arguably the most important enzyme in the animal cell plasma membrane, but the role of the membrane in its regulation is poorly understood. We investigated the relationship between Na+-K+-ATPase and membrane microdomains or ‘lipid rafts’ enriched in sulfatide (sulfogalactosyl ceramide/SGC), a glycosphingolipid implicated as a cofactor for this enzyme, in the basolateral membrane (BLM) of rainbow trout gill epithelium. Our studies demonstrated that when trout adapt to seawater (33ppt), Na+-K+-ATPase relocates to these structures. Arylsulfatase-induced desulfation of BLM SGC prevented this relocation and significantly reduced Na+-K+-ATPase activity in seawater but not freshwater trout. We contend that Na+-K+-ATPase partitions into SGC-enriched rafts to help facilitate the up-regulation of its activity during seawater adaptation. We also suggest that differential partitioning of Na+-K+-ATPase between these novel SGC-enriched regulatory platforms results in two distinct, physiological Na+ transport modes. In addition, we extend the working definition of cholesterol-dependent raft integrity to structural dependence on the sulfate moiety of SGC in this membrane.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
T. Fujii, Y. Takahashi, Y. Itomi, K. Fujita, M. Morii, Y. Tabuchi, S. Asano, K. Tsukada, N. Takeguchi, and H. Sakai
K+-Cl- Cotransporter-3a Up-regulates Na+,K+-ATPase in Lipid Rafts of Gastric Luminal Parietal Cells
J. Biol. Chem., March 14, 2008; 283(11): 6869 - 6877.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. Fuentes, C. Haond, P. M. Guerreiro, N. Silva, D. M. Power, and A. V. M. Canario
Regulation of calcium balance in the sturgeon Acipenser naccarii: a role for PTHrP
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R884 - R893.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P. Welker, B. Geist, J.-H. Fruhauf, M. Salanova, D. A. Groneberg, E. Krause, and S. Bachmann
Role of lipid rafts in membrane delivery of renal epithelial Na+-K+-ATPase, thick ascending limb
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2007; 292(3): R1328 - R1337.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
M. Kyogashima, K. Tamiya-Koizumi, T. Ehara, G. Li, R. Hu, A. Hara, T. Aoyama, and R. Kannagi
Rapid demonstration of diversity of sulfatide molecular species from biological materials by MALDI-TOF MS
Glycobiology, August 1, 2006; 16(8): 719 - 728.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.