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Papers In Press, published online ahead of print August 29, 2005
Monell Chemical Senses Center, Philadelphia, PA 19104
Corresponding Author: LHUANG{at}monell.org
Taste bud cells are epithelial cells with neuronal properties. Voltage-dependent ion channels have been physiologically described in these cells. Here we report the molecular identification and functional characterization of a voltage-gated chloride channel ClC-4 and its novel splice variant ClC-4A from taste bud cells. ClC-4A skips an exon near its 5’-end, which incurs the loss of 60 amino acids at the amino terminus. In situ hybridization and immunohistochemistry localized these two channels´ transcripts and proteins to a subset of taste bud cells. Electrophysiological recordings of the heterologously expressed channels in Xenopus oocytes showed that ClC-4 and ClC-4A had the opposite sensitivity to pH, and unique ion selectivity. The chloride channel blockers niflumic acid (NFA) and 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) had slight or no inhibitory effect on the conductance of ClC-4, but both blockers inhibited ClC-4A, suggesting that ClC-4A is a candidate channel for an acid-induced NPPB-sensitive current. Furthermore, these two channels may play a role in bitter--, sweet-- and umami--mediated taste transmission by regulating transmitter uptake into synaptic vesicles.
J. Biol. Chem, 10.1074/jbc.M507706200
Submitted on July 15, 2005
Revised on August 26, 2005
Accepted on August 29, 2005
Identification and functional characterization of a voltage-gated chloride channel and its novel splice variant in taste bud cells
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