Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on January 27, 2006
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
281/4/1868    most recent
M509465200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McFadden, D. C.
Right arrow Articles by Casadevall, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McFadden, D. C.
Right arrow Articles by Casadevall, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print November 8, 2005
J. Biol. Chem, 10.1074/jbc.M509465200
Submitted on August 26, 2005
Revised on November 3, 2005
Accepted on November 8, 2005

The physical properties of the capsular polysaccharides from cryptococcus neoformans suggest features for capsule construction

Diane C. McFadden, Magdia De Jesus, and Arturo Casadevall

Department of Medicine, Division of Infectious Disease, Albert Einstein College of Medicine, Bronx, NY 10461

Corresponding Author: casadeva{at}aecom.yu.edu

The most distinctive feature of the human pathogenic fungus is a polysaccharide capsule that is essential for virulence and is composed primarily of glucuronoxylomannan (GXM) and galactoxylomannan (GalXM). GXM mediates multiple deleterious effects on host immune function yet relatively little is known about its physical properties. The average mass of C. neoformans GXM from four antigenically different strains ranged from 1.7-7 x 106 daltons as calculated from Zimm plots of light scattering data. GalXM was significantly smaller than GXM with an average mass of 1 x 105 daltons. These molecular weights imply that GalXM is the most numerous polysaccharide in the capsule on a molar basis. The radius of gyration of the capsular polysaccharides ranged between 68 and 208 nm. Viscosity measurements suggest that neither polysaccharide altered fluid dynamics during infection since GXM behaved in solution as a polyelectrolyte and GalXM did not increase solution viscosity. Immunoblot analysis indicated heterogeneity within GXM. In agreement with this, scanning transmission electron microscopy (STEM) of GXM preparations revealed a tangled network of two different types of molecules. Mass per length measurements from light scattering and STEM were consistent and suggested GXM molecules self-associate. A mechanism for capsule construction is proposed based on the extracellular release and entanglement of GXM molecules


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
M. De Jesus, A. M. Nicola, M. L. Rodrigues, G. Janbon, and A. Casadevall
Capsular Localization of the Cryptococcus neoformans Polysaccharide Component Galactoxylomannan
Eukaryot. Cell, January 1, 2009; 8(1): 96 - 103.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. S. Klutts and T. L. Doering
Cryptococcal Xylosyltransferase 1 (Cxt1p) from Cryptococcus neoformans Plays a Direct Role in the Synthesis of Capsule Polysaccharides
J. Biol. Chem., May 23, 2008; 283(21): 14327 - 14334.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. L. Rodrigues, M. Alvarez, F. L. Fonseca, and A. Casadevall
Binding of the Wheat Germ Lectin to Cryptococcus neoformans Suggests an Association of Chitinlike Structures with Yeast Budding and Capsular Glucuronoxylomannan
Eukaryot. Cell, April 1, 2008; 7(4): 602 - 609.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. Yoneda and T. L. Doering
Regulation of Cryptococcus neoformans Capsule Size Is Mediated at the Polymer Level
Eukaryot. Cell, March 1, 2008; 7(3): 546 - 549.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. Frases, L. Nimrichter, N. B. Viana, A. Nakouzi, and A. Casadevall
Cryptococcus neoformans Capsular Polysaccharide and Exopolysaccharide Fractions Manifest Physical, Chemical, and Antigenic Differences
Eukaryot. Cell, February 1, 2008; 7(2): 319 - 327.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. L. Rodrigues, E. S. Nakayasu, D. L. Oliveira, L. Nimrichter, J. D. Nosanchuk, I. C. Almeida, and A. Casadevall
Extracellular Vesicles Produced by Cryptococcus neoformans Contain Protein Components Associated with Virulence
Eukaryot. Cell, January 1, 2008; 7(1): 58 - 67.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
L. Nimrichter, S. Frases, L. P. Cinelli, N. B. Viana, A. Nakouzi, L. R. Travassos, A. Casadevall, and M. L. Rodrigues
Self-Aggregation of Cryptococcus neoformans Capsular Glucuronoxylomannan Is Dependent on Divalent Cations
Eukaryot. Cell, August 1, 2007; 6(8): 1400 - 1410.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. S. Klutts, S. B. Levery, and T. L. Doering
A beta-1,2-Xylosyltransferase from Cryptococcus neoformans Defines a New Family of Glycosyltransferases
J. Biol. Chem., June 15, 2007; 282(24): 17890 - 17899.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
M. De Jesus, E. Hackett, M. Durkin, P. Connolly, A. Casadevall, R. Petraitiene, T. J. Walsh, and L. J. Wheat
Galactoxylomannan Does Not Exhibit Cross-Reactivity in the Platelia Aspergillus Enzyme Immunoassay
Clin. Vaccine Immunol., May 1, 2007; 14(5): 624 - 627.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
O. W. Liu, M. J. S. Kelly, E. D. Chow, and H. D. Madhani
Parallel {beta}-Helix Proteins Required for Accurate Capsule Polysaccharide Synthesis and Virulence in the Yeast Cryptococcus neoformans
Eukaryot. Cell, April 1, 2007; 6(4): 630 - 640.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. L. Rodrigues, L. Nimrichter, D. L. Oliveira, S. Frases, K. Miranda, O. Zaragoza, M. Alvarez, A. Nakouzi, M. Feldmesser, and A. Casadevall
Vesicular Polysaccharide Export in Cryptococcus neoformans Is a Eukaryotic Solution to the Problem of Fungal Trans-Cell Wall Transport
Eukaryot. Cell, January 1, 2007; 6(1): 48 - 59.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. E. Maxson, E. Dadachova, A. Casadevall, and O. Zaragoza
Radial Mass Density, Charge, and Epitope Distribution in the Cryptococcus neoformans Capsule
Eukaryot. Cell, January 1, 2007; 6(1): 95 - 109.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Yoneda and T. L. Doering
A Eukaryotic Capsular Polysaccharide Is Synthesized Intracellularly and Secreted via Exocytosis
Mol. Biol. Cell, December 1, 2006; 17(12): 5131 - 5140.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L.-A. Pirofski
Of Mice and Men, Revisited: New Insights into an Ancient Molecule from Studies of Complement Activation by Cryptococcus neoformans.
Infect. Immun., June 1, 2006; 74(6): 3079 - 3084.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement