| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print November 1, 2005
Pharmacological Sciences, SUNY Stony Brook, Stony Brook, NY 11794-8651
Corresponding Author: dan{at}pharm.sunysb.edu
Mitochondrial DNA polymerase
J. Biol. Chem, 10.1074/jbc.M509730200
Submitted on September 2, 2005
Revised on October 28, 2005
Accepted on October 31, 2005
Functional human mitochondrial DNA polymerase
forms a heterotrimer
(pol) is responsible for replication and repair of mtDNA and is mutated in individuals with genetic disorders such as chronic external opthalmoplegia and Alpers syndrome. Pol is also an adventitious target for toxic side effects of several antiviral compounds and mutation of its proofreading exonuclease leads to accelerated aging in mouse models. We have used a variety of physical and functional approaches to study the interaction of the human pol catalytic subunit with both the wild-type accessory factor, polB, and a deletion derivative that is unable to dimerize and consequently is impaired in its ability to stimulate processive DNA synthesis. Our studies clearly show that the functional human holoenzyme contains two subunits of the processivity factor and one catalytic subunit, thereby forming a heterotrimer. The structure of pol. seems to be variable, ranging from a single catalytic subunit in yeast to a heterodimer in Drosophila and a heterotrimer in mammals.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Liu, L. Qian, J.-S. Sung, N. C. de Souza-Pinto, L. Zheng, D. F. Bogenhagen, V. A. Bohr, D. M. Wilson III, B. Shen, and B. Demple Removal of Oxidative DNA Damage via FEN1-Dependent Long-Patch Base Excision Repair in Human Cell Mitochondria Mol. Cell. Biol., August 15, 2008; 28(16): 4975 - 4987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ferraris, S. Clark, E. Garelli, G. Davidzon, S. A. Moore, R. H. Kardon, R. J. Bienstock, M. J. Longley, M. Mancuso, P. Gutierrez Rios, et al. Progressive External Ophthalmoplegia and Vision and Hearing Loss in a Patient With Mutations in POLG2 and OPA1 Arch Neurol, January 1, 2008; 65(1): 125 - 131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Krishnan, L. C. Greaves, A. K. Reeve, and D. Turnbull The ageing mitochondrial genome Nucleic Acids Res., December 3, 2007; 35(22): 7399 - 7405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Loregian, E. Sinigalia, B. Mercorelli, G. Palu, and D. M. Coen Binding parameters and thermodynamics of the interaction of the human cytomegalovirus DNA polymerase accessory protein, UL44, with DNA: implications for the processivity mechanism Nucleic Acids Res., July 9, 2007; 35(14): 4779 - 4791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Farge, X. H. Pham, T. Holmlund, I. Khorostov, and M. Falkenberg The accessory subunit B of DNA polymerase {gamma} is required for mitochondrial replisome function Nucleic Acids Res., February 16, 2007; 35(3): 902 - 911. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S.L. Chan, M. J. Longley, and W. C. Copeland Modulation of the W748S mutation in DNA polymerase {gamma} by the E1143G polymorphismin mitochondrial disorders Hum. Mol. Genet., December 1, 2006; 15(23): 3473 - 3483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. DiMauro, G. Davidzon, and M. Hirano A polymorphic polymerase. Brain, July 1, 2006; 129(Pt 7): 1637 - 1639. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
