Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element

doi:10.1074/jbc.M510306200

HOME

QUICK SEARCH:

	Author:		Keyword(s):
Year:		Vol:		Page:

A more recent version of this article appeared on April 21, 2006

This Article

	Full Text (Accepted Manuscript)
	Supplemental Data
	All Versions of this Article: 281/16/10912 most recent M510306200v1
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Email this article to a friend
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Request Permissions

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Prechtel, A. T.
	Articles by Hauber, J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Prechtel, A. T.
	Articles by Hauber, J.

Social Bookmarking

	What's this?

Papers In Press, published online ahead of print February 16, 2006
J. Biol. Chem, 10.1074/jbc.M510306200
Submitted on September 20, 2005
Accepted on February 16, 2006

Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element

Alexander T. Prechtel, Jan Chemnitz, Susann Schirmer, Christina Ehlers, Ines Langbein-Detsch, Jörg Stülke, Marie-Christine Dabauvalle, Ralph H. Kehlenbach, and Joachim Hauber

Cell Biology and Virology, Heinrich-Pette-Institute for Experimental Virology and Immunology, Hamburg D-20251

Corresponding Author: joachim.hauber{at}hpi.uni-hamburg.de

Dendritic cells (DC), the most potent of the antigen-presenting cells are characterized by surface expression of CD83. Here we show that the coding region of CD83 mRNA contains a novel, cis-acting, structured RNA element that binds to HuR, a member of the ELAV family of AU-rich element (ARE) RNA binding proteins. Transient transfection of mammalian cells demonstrates that this CD83 mRNA-derived element acts as a posttranscriptional response element (PRE) in cells that overexpress HuR. Importantly, binding of HuR to the CD83 PRE does not affect mRNA stability. By using RNA interference (RNAi) it is shown that HuR mediates efficient expression of CD83. In particular, HuR is required for cytoplasmic accumulation of CD83 transcripts. Likewise, inhibition of the CRM1 nuclear export pathway by leptomycin B or overexpression of a defective form of the nucleoporin Nup214/CAN diminishes cytoplasmic CD83 mRNA levels. In summary, the presented data demonstrate that the HuR-CRM1 axis affects the nucleocytoplasmic translocation of CD83 mRNA under regular physiological conditions.

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

D. W. Shineman, B. Zhang, S. N. Leight, D. Pratico, and V. M.-Y. Lee
Thromboxane Receptor Activation Mediates Isoprostane-Induced Increases in Amyloid Pathology in Tg2576 Mice
J. Neurosci., April 30, 2008; 28(18): 4785 - 4794.
[Abstract] [Full Text] [PDF]

Z. Zhang, I. Borecki, L. Nguyen, D. Ma, K. Smith, P. C. Huettner, D. G. Mutch, T. J. Herzog, R. K. Gibb, M. A. Powell, et al.
CD83 Gene Polymorphisms Increase Susceptibility to Human Invasive Cervical Cancer
Cancer Res., December 1, 2007; 67(23): 11202 - 11208.
[Abstract] [Full Text] [PDF]

P. S. David, R. Tanveer, and J. D. Port
FRET-detectable interactions between the ARE binding proteins, HuR and p37AUF1
RNA, September 1, 2007; 13(9): 1453 - 1468.
[Abstract] [Full Text] [PDF]

Y. Kuwano, C. M. Prazma, N. Yazawa, R. Watanabe, N. Ishiura, A. Kumanogoh, H. Okochi, K. Tamaki, M. Fujimoto, and T. F. Tedder
CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells
Int. Immunol., August 1, 2007; 19(8): 977 - 992.
[Abstract] [Full Text] [PDF]

K. S. A. Khabar
Rapid transit in the immune cells: the role of mRNA turnover regulation
J. Leukoc. Biol., June 1, 2007; 81(6): 1335 - 1344.
[Abstract] [Full Text] [PDF]

B. Fries, J. Heukeshoven, I. Hauber, C. Gruttner, C. Stocking, R. H. Kehlenbach, J. Hauber, and J. Chemnitz
Analysis of Nucleocytoplasmic Trafficking of the HuR Ligand APRIL and Its Influence on CD83 Expression
J. Biol. Chem., February 16, 2007; 282(7): 4504 - 4515.
[Abstract] [Full Text] [PDF]