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Papers In Press, published online ahead of print January 17, 2006
Molecular Biology and Biotechnology, National University of Mexico, Coyoacan, Mexico City 04510
Corresponding Author: jmh{at}biomedicas.unam.mx
Cyclic diguanylic acid (c-di-GMP; cGpGp) is a global second messenger controlling motility and adhesion in bacterial cells. Intracellular concentrations of c-di-GMP depend on two opposite activities, diguanylate cyclase (DGC) recently assigned to the widespread GGDEF domain and c-di-GMP specific phosphodiesterase (PDE) associated to proteins harboring the EAL domain. To date, little is known about the targets of c-di-GMP in the cell, or if it affects transcriptional regulation of certain genes. In order to expand our knowledge of the effect of this molecule on the bacterial metabolism, here we report on the E. coli transcriptional profile under high levels of c-di-GMP. We show that an important number of genes encoding cell surface and membrane-bound proteins are altered in their transcriptional activity. On the other hand, genes encoding several transcriptional factors such as Fur, RcsA, SoxS and ZraR are upregulated and other such as GadE, GadX, GcvA, and MetR are down-regulated. Transcription of motility and cell division genes were altered, consistently with this was the physiological analysis of cells over expressing yddV, a diguanylate cyclase, these cells displayed an abnormal cell division process when high levels of c-di-GMP were present. We also show evidence that the diguanylate cyclase gene yddV is co-transcribed with dos a heme-base oxygen sensor with c-di-GMP specific phosphodiesterase activity. A
J. Biol. Chem, 10.1074/jbc.M510701200
Submitted on September 30, 2005
Revised on January 4, 2006
Accepted on January 17, 2006
Genome wide transcriptional profile of Escherichia coli in response to high levels of the second messenger c-di-GMP
dos::kan mutation rendered the cells unable to divide properly, suggesting that dos and yddV may be part of a fine-tuning mechanism for regulating the intracellular levels of c-di-GMP.
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