Functional expression of thermo-transient receptor potential channels in dental primary afferent neurons: Implication for tooth pain

doi:10.1074/jbc.M511072200

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Papers In Press, published online ahead of print April 4, 2006
J. Biol. Chem, 10.1074/jbc.M511072200
Submitted on October 12, 2005
Accepted on April 3, 2006

Functional expression of thermo-transient receptor potential channels in dental primary afferent neurons: Implication for tooth pain

Chul-Kyu Park, Mi Sun Kim, Zhi Fang, Hai Ying Li, Sung Jun Jung, Se-Young Choi, Sung Joong Lee, Kyungpyo Park, Joong Soo Kim, and Seog Bae Oh

Department of Physiology, College of Dentistry and Dental Research Institute, Seoul 110-749

Corresponding Author: odolbae{at}snu.ac.kr

Temperature signaling can be initiated by members of transient receptor potential family (thermo-TRP) channels. Hot and cold substances applied to teeth usually elicit pain sensation. This study investigated the expression of thermo-TRP channels in dental primary afferent neurons of the rat identified by retrograde labeling with a fluorescent dye in maxillary molars. Single-cell RT-PCR and immuno- histochemistry revealed expression of TRPV1, TRPM8 and TRPA1 in subsets of such neurons. Capsaicin (a TRPV1 agonist), menthol (a TRPM8 agonist) and icilin (a TRPM8 and TRPA1 agonist) increased intracellular calcium and evoked cationic currents in subsets of neurons, as did the appropriate temperature changes (>43oC, <25oC and <17oC respectively). Some neurons expressed more than one TRP channel and responded to two or three corresponding stimuli (ligands or thermal stimuli). Immunohistochemistry and single-cell RT-PCR following whole-cell recordings provided direct evidence on the association between the responsiveness to thermo-TRP ligands and expression of thermo-TRP channels. The results suggest that activation of thermo-TRP channels expressed by dental afferent neurons contributes to tooth pain evoked by temperature stimuli. Accordingly, blockade of thermo-TRP channels will provide a novel therapeutic intervention for the treatment of tooth pain.

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