|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print February 15, 2006
Department of Microbiology, Institute of Molecular Biological Sciences, Amsterdam 1081 HV
Corresponding Author: luirink{at}bio.vu.nl
Inner membrane proteins (IMPs) of Escherichia coli use different pathways for membrane targeting and integration. YidC plays an essential but poorly defined role in the integration and folding of IMPs both in conjunction with the Sec-translocon and as a Sec-independent insertase. Depletion of YidC only marginally affects the insertion of Sec-dependent IMPs, whereas it blocks the insertion of a subset of Sec-independent IMPs. Substrates of this latter “YidC-only” pathway include the relatively small IMPs M13 procoat, Pf3 coat protein and subunit c of the F1F0 ATPase. Recently, it has been shown that the steady state level of the larger and more complex CyoA subunit of the cytochrome o oxidase is also severely affected upon depletion of YidC. In the present study, we have analyzed the biogenesis of the integral lipoprotein CyoA. Collectively, our data suggest that the first transmembrane segment (TM) of CyoA rather than the signal sequence recruits the SRP for membrane targeting. Membrane integration and assembly appears to occur in two distinct sequential steps. YidC is sufficient to catalyze insertion of the N-terminal domain consisting of the signal sequence, TM1 and the small periplasmic domain in between. Translocation of the large C-terminal periplasmic domain requires the Sec-translocon and SecA suggesting that for this particular IMP, the Sec-translocon might operate downstream of YidC.
J. Biol. Chem, 10.1074/jbc.M511357200
Submitted on October 19, 2005
Revised on January 26, 2006
Accepted on February 15, 2006
Distinct requirements for translocation of the N-tail and C-tail of the Escherichia coli inner membrane protein CYOA
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Yu, G. Koningstein, A. Pop, and J. Luirink The Conserved Third Transmembrane Segment of YidC Contacts Nascent Escherichia coli Inner Membrane Proteins J. Biol. Chem., December 12, 2008; 283(50): 34635 - 34642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kol, N. Nouwen, and A. J. M. Driessen Mechanisms of YidC-mediated Insertion and Assembly of Multimeric Membrane Protein Complexes J. Biol. Chem., November 14, 2008; 283(46): 31269 - 31273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Price and A. J. M. Driessen YidC Is Involved in the Biogenesis of Anaerobic Respiratory Complexes in the Inner Membrane of Escherichia coli J. Biol. Chem., October 3, 2008; 283(40): 26921 - 26927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Baars, S. Wagner, D. Wickstrom, M. Klepsch, A. J. Ytterberg, K. J. van Wijk, and J.-W. de Gier Effects of SecE Depletion on the Inner and Outer Membrane Proteomes of Escherichia coli J. Bacteriol., May 15, 2008; 190(10): 3505 - 3525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ravaud, G. Stjepanovic, K. Wild, and I. Sinning The Crystal Structure of the Periplasmic Domain of the Escherichia coli Membrane Protein Insertase YidC Contains a Substrate Binding Cleft J. Biol. Chem., April 4, 2008; 283(14): 9350 - 9358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Dong, S. R. Palmer, A. Hasona, S. Nagamori, H. R. Kaback, R. E. Dalbey, and L. J. Brady Functional Overlap but Lack of Complete Cross-Complementation of Streptococcus mutans and Escherichia coli YidC Orthologs J. Bacteriol., April 1, 2008; 190(7): 2458 - 2469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-J. Scheffers, C. Robichon, G. J. Haan, T. den Blaauwen, G. Koningstein, E. van Bloois, J. Beckwith, and J. Luirink Contribution of the FtsQ Transmembrane Segment to Localization to the Cell Division Site J. Bacteriol., October 15, 2007; 189(20): 7273 - 7280. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. L. Fiumera, S. A. Broadley, and T. D. Fox Translocation of Mitochondrially Synthesized Cox2 Domains from the Matrix to the Intermembrane Space Mol. Cell. Biol., July 1, 2007; 27(13): 4664 - 4673. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Jia, M. K. Dienhart, and R. A. Stuart Oxa1 Directly Interacts with Atp9 and Mediates Its Assembly into the Mitochondrial F1Fo-ATP Synthase Complex Mol. Biol. Cell, May 1, 2007; 18(5): 1897 - 1908. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Nouet, M. Bourens, O. Hlavacek, S. Marsy, C. Lemaire, and G. Dujardin Rmd9p Controls the Processing/Stability of Mitochondrial mRNAs and Its Overexpression Compensates for a Partial Deficiency of Oxa1p in Saccharomyces cerevisiae Genetics, March 1, 2007; 175(3): 1105 - 1115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-i. Nishiyama, A. Ikegami, M. Moser, E. Schiltz, H. Tokuda, and M. Muller A Derivative of Lipid A Is Involved in Signal Recognition Particle/SecYEG-dependent and -independent Membrane Integrations J. Biol. Chem., November 24, 2006; 281(47): 35667 - 35676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kol, B. R. Turrell, J. de Keyzer, M. van der Laan, N. Nouwen, and A. J. M. Driessen YidC-mediated Membrane Insertion of Assembly Mutants of Subunit c of the F1F0 ATPase J. Biol. Chem., October 6, 2006; 281(40): 29762 - 29768. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |