The critical roles of polyamines in regulating ColE7 production and restricting ColE7 uptake of the colicin producing Escherichia coli

doi:10.1074/jbc.M511365200

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Papers In Press, published online ahead of print March 20, 2006
J. Biol. Chem, 10.1074/jbc.M511365200
Submitted on October 19, 2005
Revised on February 27, 2006
Accepted on March 20, 2006

The critical roles of polyamines in regulating ColE7 production and restricting ColE7 uptake of the colicin producing Escherichia coli

Yi-Hsuan Pan, Chen-Chung Liao, Chou-Chiang Kuo, Kow-Jen Duan, Po-Huang Liang, Hanna S. Yuan, Shiau-Ting Hu, and Kin-Fu Chak

Biochemistry Dept., National Yang Ming University, Taipei, Taiwan 11221

Corresponding Author: kfchak{at}ym.edu.tw

The ColE7 operon is an SOS response regulon, which encodes bacteriocin ColE7 to kill susceptible E. coli and its related enterobacteria under conditions of stress. We have observed for the first time that polyamines confer limited resistance against ColE7 on E. coli cells. Thus, this study aims to investigate the role of polyamines in modulating the protective effect of the E. coli cells against colicin. In the experiments, we surprisingly found that endogenous polyamines are also essential for ColE7 production and the rate of polyamine synthesis is directly related to the SOS response. Our experimental results further indicated that exogenous polyamines suppress the expression of TolA, BtuB, OmpF and OmpC proteins that are responsible for ColE7 uptake. Moreover, two-dimensional gel electrophoresis revealed that the production of two periplasmic proteins, PotD and OppA, is increased in E. coli cells under ColE7 exposure. Based on these observations, we propose that endogenous polyamines may play a dual role in ColE7 system. Polyamines may participate in initiating the expression of the SOS response of the ColE7 operon, and simultaneously down regulate proteins that are essential for colicin uptake, thus conferring a survival advantage on colicin producing E. coli under stress conditions in the natural environment.

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