Plasticity in the composition of the light harvesting antenna of higher plants preserves structural integrity and biological function

doi:10.1074/jbc.M511415200

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Papers In Press, published online ahead of print March 21, 2006
J. Biol. Chem, 10.1074/jbc.M511415200
Submitted on October 20, 2005
Revised on February 22, 2006
Accepted on March 21, 2006

Plasticity in the composition of the light harvesting antenna of higher plants preserves structural integrity and biological function

Alexander V. Ruban, Svetlana Solovieva, Pamela J. Lee, Cristian Ilioaia, Mark Wentworth, Ulrika Ganeteg, Frank Klimmek, Wah Soon Chow, Jan M. Anderson, Stefan Jansson, and Peter Horton

Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN

Corresponding Author: a.ruban{at}sheffield.ac.uk

Arabidopsis plants in which the major trimeric light harvesting complex (LHCIIb) is eliminated by antisense expression still exhibit the typical macrostructure of photosystem II in the granal membranes. Here the detailed analysis of the composition and the functional state of the light harvesting antennae of both photosystem I and II of these plants is presented. Two new populations of trimers were found, both functional in energy transfer to the PSII reaction centre, a homotrimer of CP26 and a heterotrimer of CP26 and Lhcb3. These trimers possess characteristic features thought to be specific for the native LHCIIb trimers they are replacing: the long-wavelength form of lutein and at least 1 extra chlorophyll b, but they were less stable. A new population of loosely bound LHCI was also found, contributing to an increased antenna size for photosystem I, which may in part compensate for the loss of the phosphorylated LHCIIb that can associate with this photosystem. Thus, the loss of LHCIIb has triggered concerted compensatory responses in the composition of antennae of both photosystems. These responses clearly show the importance of LHCIIb in the structure and assembly of the photosynthetic membrane and illustrate the extreme plasticity at the level of the composition of the light harvesting system.

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