p204 protein is required for the differentiation of P19 murine embryonal carcinoma cells to beating cardiac myocytes.  Its expression is activated by the cardiac Gata4, Nkx2.5 and Tbx5 proteins

doi:10.1074/jbc.M511747200

HOME

QUICK SEARCH:

	Author:		Keyword(s):
Year:		Vol:		Page:

A more recent version of this article appeared on May 26, 2006

This Article

	Full Text (Accepted Manuscript)
	Supplemental Data
	All Versions of this Article: 281/21/14882 most recent M511747200v1
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Email this article to a friend
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Request Permissions

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Ding, B.
	Articles by Lengyel, P.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Ding, B.
	Articles by Lengyel, P.

Social Bookmarking

	What's this?

Papers In Press, published online ahead of print March 22, 2006
J. Biol. Chem, 10.1074/jbc.M511747200
Submitted on October 31, 2005
Accepted on March 22, 2006

p204 protein is required for the differentiation of P19 murine embryonal carcinoma cells to beating cardiac myocytes. Its expression is activated by the cardiac Gata4, Nkx2.5 and Tbx5 proteins

Bo Ding, Chuan-ju Liu, Yan Huang, Reed P. Hickey, Jin Yu, Weihua Kong, and Peter Lengyel

MB&B, Yale University School of Medicine, New Haven, CT 06520-8024

Corresponding Author: peter.lengyel{at}yale.edu

Among 10 adult mouse tissues tested, the p204 protein levels were highest in heart and skeletal muscle. We described earlier that the MyoD-inducible p204 protein is required for the differentiation of cultured murine C2C12 skeletal muscle myoblasts to myotubes. Here we report that p204 was also required for the differentiation of cultured P19 murine embryonal carcinoma stem cells to beating cardiac myocytes. As shown by others this process can be triggered by dimethylsulfoxide (DMSO). We established that DMSO induced the formation of 204RNA and p204. Ectopic p204 could partially substitute for DMSO in inducing differentiation, whereas ectopic 204 antisense RNA inhibited the differentiation. Experiments with reporter constructs including regulatory regions from the Ifi204 gene (encoding p204) in P19 cells and in cultured newborn rat cardiac myocytes, as well as chromatin coimmunoprecipitations with transcription factors, revealed that p204 expression was synergistically transactivated by the cardiac Gata4, Nkx2.5 and Tbx5 transcription factors. Furthermore ectopic p204 triggered the expression of Gata4 and Nkx2.5 in P19 cells. p204 contains a Nuclear Export Signal (NES) and was partially translocated to the cytoplasm during the differentiation. p204 from which the NES was deleted was not translocated, and it did not induce differentiation. The various mechanisms by which p204 promoted the differentiation are reported in an accompanying article.

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

A. Rojas, S. W. Kong, P. Agarwal, B. Gilliss, W. T. Pu, and B. L. Black
GATA4 Is a Direct Transcriptional Activator of Cyclin D2 and Cdk4 and Is Required for Cardiomyocyte Proliferation in Anterior Heart Field-Derived Myocardium
Mol. Cell. Biol., September 1, 2008; 28(17): 5420 - 5431.
[Abstract] [Full Text] [PDF]

Y. Luan, X.-P. Yu, N. Yang, S. Frenkel, L. Chen, and C.-j. Liu
p204 Protein Overcomes the Inhibition of Core Binding Factor {alpha}-1-mediated Osteogenic Differentiation by Id Helix-Loop-Helix Proteins
Mol. Biol. Cell, May 1, 2008; 19(5): 2113 - 2126.
[Abstract] [Full Text] [PDF]

B. Ding and P. Lengyel
p204 Protein Is a Novel Modulator of Ras Activity
J. Biol. Chem., February 29, 2008; 283(9): 5831 - 5848.
[Abstract] [Full Text] [PDF]

Y. Luan, X.-P. Yu, K. Xu, B. Ding, J. Yu, Y. Huang, N. Yang, P. Lengyel, P. E. Di Cesare, and C.-j. Liu
The Retinoblastoma Protein Is an Essential Mediator of Osteogenesis That Links the p204 Protein to the Cbfa1 Transcription Factor Thereby Increasing Its Activity
J. Biol. Chem., June 8, 2007; 282(23): 16860 - 16870.
[Abstract] [Full Text] [PDF]

B. Ding, C.-j. Liu, Y. Huang, J. Yu, W. Kong, and P. Lengyel
p204 Protein Overcomes the Inhibition of the Differentiation of P19 Murine Embryonal Carcinoma Cells to Beating Cardiac Myocytes by Id Proteins
J. Biol. Chem., May 26, 2006; 281(21): 14893 - 14906.
[Abstract] [Full Text] [PDF]