Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on April 21, 2006
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
281/16/11332    most recent
M512199200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marinissen, M. J.
Right arrow Articles by Cuadrado, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marinissen, M. J.
Right arrow Articles by Cuadrado, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print February 13, 2006
J. Biol. Chem, 10.1074/jbc.M512199200
Submitted on November 14, 2005
Accepted on February 13, 2006

Inhibition of heme oxygenase-1 interferes with the transforming activity of the Kaposi's sarcoma herpesvirus-encoded G protein-coupled receptor

Maria Julia Marinissen, Tamara T. Tanos, Marta Bolós, Maria Rosa de Sagarra, Omar A. Coso, and Antonio Cuadrado

Instituto de Investigaciones Biomédicas A. Sols UAM-CSIC, Departamento de Bioquímica, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Madrid 28029

Corresponding Author: mjmarinissen{at}iib.uam.es

Heme oxygenase-1 (HO-1), the inducible enzyme responsible for the rate-limiting step in the heme catabolism, is expressed in AIDS-Kaposi’s sarcoma (KS) lesions. Its expression is upregulated by the Kaposi’s sarcoma-associated herpesvirus (KSHV) in endothelial cells but the mechanisms underlying KSHV-induced HO-1 expression are still unknown. In this study we investigated whether the oncogenic G protein-coupled receptor (KSHV-GPCR or vGPCR), one of the key KSHV genes involved in KS development, activated HO-1 expression. Here we show that vGPCR induces HO-1 mRNA and protein levels in fibroblasts and endothelial cells. Moreover, targeted knock-down gene expression of HO-1 by shRNA and chemical inhibition of HO-1 enzymatic activity by Sn protoporphyrin IX (SnPP), impaired vGPCR-induced survival, proliferation, transformation, and VEGF-A expression. vGPCR-expressing cells implanted in the dorsal flank of nude mice developed tumors with elevated HO-1 expression and activity. Chronic administration of SnPP to the implanted mice, under conditions that effectively blocked HO-1 activity and VEGF-A expression in the transplanted cells, strikingly reduced tumor growth, without apparent side effects. On the contrary, administration of the HO-1 inducer Co protoporphyrin (CoPP), further enhanced vGPCR-induced tumor growth. These data postulate HO-1 as an important mediator of vGPCR-induced tumor growth and suggest that inhibition of intratumoral HO-1 activity by SnPP may be a potential therapeutic strategy.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
C. I. Schwer, A. M. Guerrero, M. Humar, M. Roesslein, U. Goebel, P. Stoll, K. K. Geiger, B. H. J. Pannen, A. Hoetzel, and R. Schmidt
Heme Oxygenase-1 Inhibits the Proliferation of Pancreatic Stellate Cells by Repression of the Extracellular Signal-Regulated Kinase1/2 Pathway
J. Pharmacol. Exp. Ther., December 1, 2008; 327(3): 863 - 871.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. G. Innamorato, A. I. Rojo, A. J. Garcia-Yague, M. Yamamoto, M. L. de Ceballos, and A. Cuadrado
The Transcription Factor Nrf2 Is a Therapeutic Target against Brain Inflammation
J. Immunol., July 1, 2008; 181(1): 680 - 689.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
J. Dulak, J. Deshane, A. Jozkowicz, and A. Agarwal
Heme Oxygenase-1 and Carbon Monoxide in Vascular Pathobiology: Focus on Angiogenesis
Circulation, January 15, 2008; 117(2): 231 - 241.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
N. Canas, T. Valero, M. Villarroya, E. Montell, J. Verges, A. G. Garcia, and M. G. Lopez
Chondroitin Sulfate Protects SH-SY5Y Cells from Oxidative Stress by Inducing Heme Oxygenase-1 via Phosphatidylinositol 3-Kinase/Akt
J. Pharmacol. Exp. Ther., December 1, 2007; 323(3): 946 - 953.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. L. Skalsky, M. A. Samols, K. B. Plaisance, I. W. Boss, A. Riva, M. C. Lopez, H. V. Baker, and R. Renne
Kaposi's Sarcoma-Associated Herpesvirus Encodes an Ortholog of miR-155
J. Virol., December 1, 2007; 81(23): 12836 - 12845.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. J. Martin, T. Tanos, A. B. Garcia, D. Martin, J. S. Gutkind, O. A. Coso, and M. J. Marinissen
The G{alpha}12/13 Family of Heterotrimeric G Proteins and the Small GTPase RhoA Link the Kaposi Sarcoma-associated Herpes Virus G Protein-coupled Receptor to Heme Oxygenase-1 Expression and Tumorigenesis
J. Biol. Chem., November 23, 2007; 282(47): 34510 - 34524.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
P. Larghero, R. Vene, S. Minghelli, G. Travaini, M. Morini, N. Ferrari, U. Pfeffer, D. M. Noonan, A. Albini, and R. Benelli
Biological assays and genomic analysis reveal lipoic acid modulation of endothelial cell behavior and gene expression
Carcinogenesis, May 1, 2007; 28(5): 1008 - 1020.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
H. Was, T. Cichon, R. Smolarczyk, D. Rudnicka, M. Stopa, C. Chevalier, J. J. Leger, B. Lackowska, A. Grochot, K. Bojkowska, et al.
Overexpression of Heme Oxygenase-1 in Murine Melanoma: Increased Proliferation and Viability of Tumor Cells, Decreased Survival of Mice
Am. J. Pathol., December 1, 2006; 169(6): 2181 - 2198.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement