Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on March 10, 2006
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
281/10/6246    most recent
M512705200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Senga, T.
Right arrow Articles by Dutta, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Senga, T.
Right arrow Articles by Dutta, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print January 9, 2006
J. Biol. Chem, 10.1074/jbc.M512705200
Submitted on November 28, 2005
Revised on January 9, 2006
Accepted on January 9, 2006

PCNA is a co-factor for Cdt1 degradation by CUL4/DDB1 mediated N-terminal ubiquitination

Takeshi Senga, Umasundari Sivaprasad, Wenge Zhu, Jong Hoon Park, Emily E. Arias, Johannes C. Walter, and Anindya Dutta

Biochemistry and Mol. Gen., University of Virginia Health Sciences System, Charlottesville, VA 22908

Corresponding Author: ad8q{at}virginia.edu

Cdt1, a protein essential in G1 for licensing of origins for DNA replication, is inhibited in S phase both by binding to geminin and degradation by proteasomes. Cdt1 is also degraded after DNA damage in order to stop licensing of new origins until after DNA repair. Phosphorylation of Cdt1 by cyclin dependent kinases promotes its binding to Skp2-SCF E3 ubiquitin ligase, but the Cdk2/Skp2 mediated pathway is not essential for the degradation of Cdt1. Here we show that the N-terminus of Cdt1 contains a second degradation signal that is active after DNA damage and in S-phase and is dependent on the interaction of Cdt1 with PCNA through a PCNA binding motif. The degradation involves N terminal ubiquitination and requires Cul4 and Ddb1 proteins, components of an E3 ubiquitin ligase implicated in protein degradation after DNA damage. Therefore PCNA, the match-maker for many proteins involved in DNA and chromatin metabolism, also serves to promote the targeted degradation of associated proteins in S phase or after DNA damage.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Su'etsugu, K. Nakamura, K. Keyamura, Y. Kudo, and T. Katayama
Hda Monomerization by ADP Binding Promotes Replicase Clamp-mediated DnaA-ATP Hydrolysis
J. Biol. Chem., December 26, 2008; 283(52): 36118 - 36131.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Nishitani, Y. Shiomi, H. Iida, M. Michishita, T. Takami, and T. Tsurimoto
CDK Inhibitor p21 Is Degraded by a Proliferating Cell Nuclear Antigen-coupled Cul4-DDB1Cdt2 Pathway during S Phase and after UV Irradiation
J. Biol. Chem., October 24, 2008; 283(43): 29045 - 29052.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
T. Abbas, U. Sivaprasad, K. Terai, V. Amador, M. Pagano, and A. Dutta
PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex
Genes & Dev., September 15, 2008; 22(18): 2496 - 2506.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Y. Kim, N. G. Starostina, and E. T. Kipreos
The CRL4Cdt2 ubiquitin ligase targets the degradation of p21Cip1 to control replication licensing
Genes & Dev., September 15, 2008; 22(18): 2507 - 2519.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. R. Hall, H. O. Lee, B. D. Bunker, E. S. Dorn, G. C. Rogers, R. J. Duronio, and J. G. Cook
Cdt1 and Cdc6 Are Destabilized by Rereplication-induced DNA Damage
J. Biol. Chem., September 12, 2008; 283(37): 25356 - 25363.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Guerrero-Santoro, M. G. Kapetanaki, C. L. Hsieh, I. Gorbachinsky, A. S. Levine, and V. Rapic-Otrin
The Cullin 4B-Based UV-Damaged DNA-Binding Protein Ligase Binds to UV-Damaged Chromatin and Ubiquitinates Histone H2A
Cancer Res., July 1, 2008; 68(13): 5014 - 5022.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. K. Teer and A. Dutta
Human Cdt1 Lacking the Evolutionarily Conserved Region That Interacts with MCM2-7 Is Capable of Inducing Re-replication
J. Biol. Chem., March 14, 2008; 283(11): 6817 - 6825.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. Sugimoto, I. Kitabayashi, S. Osano, Y. Tatsumi, T. Yugawa, M. Narisawa-Saito, A. Matsukage, T. Kiyono, and M. Fujita
Identification of Novel Human Cdt1-binding Proteins by a Proteomics Approach: Proteolytic Regulation by APC/CCdh1
Mol. Biol. Cell, March 1, 2008; 19(3): 1007 - 1021.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Stoyanova, T. Yoon, D. Kopanja, M. B. Mokyr, and P. Raychaudhuri
The Xeroderma Pigmentosum Group E Gene Product DDB2 Activates Nucleotide Excision Repair by Regulating the Level of p21Waf1/Cip1
Mol. Cell. Biol., January 1, 2008; 28(1): 177 - 187.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. Tardat, R. Murr, Z. Herceg, C. Sardet, and E. Julien
PR-Set7 dependent lysine methylation ensures genome replication and stability through S phase
J. Cell Biol., December 31, 2007; 179(7): 1413 - 1426.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
W. Zhu, C. Ukomadu, S. Jha, T. Senga, S. K. Dhar, J. A. Wohlschlegel, L. K. Nutt, S. Kornbluth, and A. Dutta
Mcm10 and And-1/CTF4 recruit DNA polymerase {alpha} to chromatin for initiation of DNA replication
Genes & Dev., September 15, 2007; 21(18): 2288 - 2299.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Wen, K. M. Duus, T. D. Friedrich, and C. M. C. de Noronha
The HIV1 Protein Vpr Acts to Promote G2 Cell Cycle Arrest by Engaging a DDB1 and Cullin4A-containing Ubiquitin Ligase Complex Using VprBP/DCAF1 as an Adaptor
J. Biol. Chem., September 14, 2007; 282(37): 27046 - 27057.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. R. Hall, E. Kow, K. R. Nevis, C. K. Lu, K. S. Luce, Q. Zhong, and J. G. Cook
Cdc6 Stability Is Regulated by the Huwe1 Ubiquitin Ligase after DNA Damage
Mol. Biol. Cell, September 1, 2007; 18(9): 3340 - 3350.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
E. E. Arias and J. C. Walter
Strength in numbers: preventing rereplication via multiple mechanisms in eukaryotic cells
Genes & Dev., March 1, 2007; 21(5): 497 - 518.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. L. Kerns, S. J. Torke, J. M. Benjamin, and T. J. McGarry
Geminin Prevents Rereplication during Xenopus Development
J. Biol. Chem., February 23, 2007; 282(8): 5514 - 5521.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Kim and E. T. Kipreos
The Caenorhabditis elegans Replication Licensing Factor CDT-1 Is Targeted for Degradation by the CUL-4/DDB-1 Complex
Mol. Cell. Biol., February 15, 2007; 27(4): 1394 - 1406.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. Shibutani, L. M. Swanhart, and R. J. Duronio
Rbf1-independent termination of E2f1-target gene expression during early Drosophila embryogenesis
Development, February 1, 2007; 134(3): 467 - 478.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
C. L. Sansam, J. L. Shepard, K. Lai, A. Ianari, P. S. Danielian, A. Amsterdam, N. Hopkins, and J. A. Lees
DTL/CDT2 is essential for both CDT1 regulation and the early G2/M checkpoint
Genes & Dev., November 15, 2006; 20(22): 3117 - 3129.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. A. Lovejoy, K. Lock, A. Yenamandra, and D. Cortez
DDB1 Maintains Genome Integrity through Regulation of Cdt1
Mol. Cell. Biol., November 1, 2006; 26(21): 7977 - 7990.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
W. A. Braden, J. M. Lenihan, Z. Lan, K. S. Luce, W. Zagorski, E. Bosco, M. F. Reed, J. G. Cook, and E. S. Knudsen
Distinct Action of the Retinoblastoma Pathway on the DNA Replication Machinery Defines Specific Roles for Cyclin-Dependent Kinase Complexes in Prereplication Complex Assembly and S-Phase Progression
Mol. Cell. Biol., October 15, 2006; 26(20): 7667 - 7681.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Y. Tatsumi, N. Sugimoto, T. Yugawa, M. Narisawa-Saito, T. Kiyono, and M. Fujita
Deregulation of Cdt1 induces chromosomal damage without rereplication and leads to chromosomal instability
J. Cell Sci., August 1, 2006; 119(15): 3128 - 3140.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Das-Bradoo, R. M. Ricke, and A.-K. Bielinsky
Interaction between PCNA and Diubiquitinated Mcm10 Is Essential for Cell Growth in Budding Yeast.
Mol. Cell. Biol., July 1, 2006; 26(13): 4806 - 4817.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement