Hyaluronan synthesis induces microvillus-like cell surface protrusions

doi:10.1074/jbc.M512840200

HOME

QUICK SEARCH:

	Author:		Keyword(s):
Year:		Vol:		Page:

A more recent version of this article appeared on June 9, 2006

This Article

	Full Text (Accepted Manuscript)
	Supplemental Data
	All Versions of this Article: 281/23/15821 most recent M512840200v1
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Email this article to a friend
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Request Permissions

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Kultti, A.
	Articles by Tammi, M. I.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Kultti, A.
	Articles by Tammi, M. I.

Social Bookmarking

	What's this?

Papers In Press, published online ahead of print April 4, 2006
J. Biol. Chem, 10.1074/jbc.M512840200
Submitted on December 1, 2005
Revised on March 21, 2006
Accepted on March 31, 2006

Hyaluronan synthesis induces microvillus-like cell surface protrusions

Anne Kultti, Kirsi Rilla, Riikka Tiihonen, Andrew P. Spicer, Raija H. Tammi, and Markku I. Tammi

Department of Anatomy, University of Kuopio, Kuopio FIN-70211

Corresponding Author: tammi{at}uku.fi

Hyaluronan synthases (HASs) are plasma membrane enzymes that simultaneously elongate, bind and extrude the growing hyaluronan chain directly into extracellular space. In cells transfected with GFP-tagged Has3, the dorsal surface was decorated by up to 150 slender, 3-20 µm long microvillus-type plasma membrane protrusions, which also contained filamentous actin, the hyaluronan receptor CD44, and lipid raft microdomains. Enzymatic activity of HAS was required for the growth of the microvilli, not present in cells transfected with other GFP proteins or inactive GFP-Has3 mutants, or in cells incubated with exogenous soluble hyaluronan. The microvilli induced by HAS3 gradually withered by introduction of an inhibitor of hyaluronan synthesis, and rapidly retracted by hyaluronidase digestion, while they were not affected by competition with hyaluronan oligosaccharides, and disruption of the CD44 gene, suggesting independence of hyaluronan receptors. The data brings out the novel concept that the glycocalyx created by dense arrays of hyaluronan chains, tethered to HAS during biosynthesis, can induce and maintain prominent microvilli.

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

S. W. Sakr, S. Potter-Perigo, M. G. Kinsella, P. Y. Johnson, K. R. Braun, Y. Goueffic, M. E. Rosenfeld, and T. N. Wight
Hyaluronan Accumulation Is Elevated in Cultures of Low Density Lipoprotein Receptor-deficient Cells and Is Altered by Manipulation of Cell Cholesterol Content
J. Biol. Chem., December 26, 2008; 283(52): 36195 - 36204.
[Abstract] [Full Text] [PDF]

K. Rilla, R. Tiihonen, A. Kultti, M. Tammi, and R. Tammi
Pericellular Hyaluronan Coat Visualized in Live Cells With a Fluorescent Probe Is Scaffolded by Plasma Membrane Protrusions
J. Histochem. Cytochem., October 1, 2008; 56(10): 901 - 910.
[Abstract] [Full Text] [PDF]

T. Fujiwara, T. Kawakatsu, S. Tayama, Y. Kobayashi, N. Sugiura, K. Kimata, and Y. Takai
Hyaluronan-CD44 pathway regulates orientation of mitotic spindle in normal epithelial cells.
Genes Cells, July 1, 2008; 13(7): 759 - 770.
[Abstract] [Full Text] [PDF]

S. Misra, L. M. Obeid, Y. A. Hannun, S. Minamisawa, F. G. Berger, R. R. Markwald, B. P. Toole, and S. Ghatak
Hyaluronan Constitutively Regulates Activation of COX-2-mediated Cell Survival Activity in Intestinal Epithelial and Colon Carcinoma Cells
J. Biol. Chem., May 23, 2008; 283(21): 14335 - 14344.
[Abstract] [Full Text] [PDF]

S. Meran, D. W. Thomas, P. Stephens, S. Enoch, J. Martin, R. Steadman, and A. O. Phillips
Hyaluronan Facilitates Transforming Growth Factor-{beta}1-mediated Fibroblast Proliferation
J. Biol. Chem., March 7, 2008; 283(10): 6530 - 6545.
[Abstract] [Full Text] [PDF]

S. Meran, D. Thomas, P. Stephens, J. Martin, T. Bowen, A. Phillips, and R. Steadman
Involvement of Hyaluronan in Regulation of Fibroblast Phenotype
J. Biol. Chem., August 31, 2007; 282(35): 25687 - 25697.
[Abstract] [Full Text] [PDF]