|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print December 28, 2005
Cancer Immunology and AIDS, St. Vincent’s Institute of Medical, Fitzroy, Victoria 02115
Corresponding Author: rik{at}medstv.unimelb.edu.au
Type-I collagen (Col I)-stimulated matrix metalloproteinase-2 (MMP-2) activation via membrane-type 1 MMP (MT1-MMP) involves both a transcriptional increase in MT1-MMP expression, and a non-transcriptional response mediated by pre-existing MT1-MMP. In order to identify which MT1-MMP domains were required for the non-transcriptional response, MCF-7 cells that lack endogenous MT1-MMP were transfected with either wild-type or domain mutant MT1-MMP constructs. We observed that mutant constructs lacking the MT1-MMP cytoplasmic tail (CT) were able to activate MMP-2 in response to Col I, but not a construct lacking the MT1-MMP hemopexin domain. Col I did not alter total MT1-MMP protein levels, nor did it appear to directly induce MT1-MMP oligomerization. Col I did however redistribute pre-existing MT1-MMP to the cell periphery compared to unstimulated cells that displayed a more diffuse staining pattern. In addition, Col I blocked the internalization of MT1-MMP in a dynamin-dependent manner via clathrin-coated pit mediated endocytosis. This mechanism of impaired internalization is different to that reported for concanavalin A as it is not mediated by the CT of MT1-MMP but rather by the hemopexin domain. In summary, upon Col I binding to its cell surface receptor, MT1-MMP internalization via clathrin-coated pit-mediated endocytosis is impaired through interactions with the hemopexin domain, thereby regulating its function and ability to activate MMP-2.
J. Biol. Chem, 10.1074/jbc.M513084200
Submitted on December 7, 2005
Accepted on December 28, 2005
Type-I collagen abrogates the clathrin-mediated internalization of membrane-type-1 matrix metalloproteinase (MT1-MMP) via the MT1-MMP hemopexin domain
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Sakurai-Yageta, C. Recchi, G. Le Dez, J.-B. Sibarita, L. Daviet, J. Camonis, C. D'Souza-Schorey, and P. Chavrier The interaction of IQGAP1 with the exocyst complex is required for tumor cell invasion downstream of Cdc42 and RhoA J. Cell Biol., October 21, 2008; 181(6): 985 - 998. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhong and Y. Chau Antitumor activity of a membrane lytic peptide cyclized with a linker sensitive to membrane type 1-matrix metalloproteinase Mol. Cancer Ther., September 1, 2008; 7(9): 2933 - 2940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-Y. Li, I. Ota, I. Yana, F. Sabeh, and S. J. Weiss Molecular Dissection of the Structural Machinery Underlying the Tissue-invasive Activity of Membrane Type-1 Matrix Metalloproteinase Mol. Biol. Cell, August 1, 2008; 19(8): 3221 - 3233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-A. Cho, P. Osenkowski, H. Zhao, S. Kim, M. Toth, K. Cole, A. Aboukameel, A. Saliganan, L. Schuger, R. D. Bonfil, et al. The Inactive 44-kDa Processed Form of Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) Enhances Proteolytic Activity via Regulation of Endocytosis of Active MT1-MMP J. Biol. Chem., June 20, 2008; 283(25): 17391 - 17405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Fortier, M. Touaibia, S. Lord-Dufour, J. Galipeau, R. Roy, and B. Annabi Tetra- and hexavalent mannosides inhibit the pro-apoptotic, antiproliferative and cell surface clustering effects of concanavalin-A: Impact on MT1-MMP functions in marrow-derived mesenchymal stromal cells Glycobiology, February 1, 2008; 18(2): 195 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. Ramsay, M. D. Keppler, M. Jazayeri, G. J. Thomas, M. Parsons, S. Violette, P. Weinreb, I. R. Hart, and J. F. Marshall HS1-Associated Protein X-1 Regulates Carcinoma Cell Migration and Invasion via Clathrin-Mediated Endocytosis of Integrin {alpha}v{beta}6 Cancer Res., June 1, 2007; 67(11): 5275 - 5284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Yana, H. Sagara, S. Takaki, K. Takatsu, K. Nakamura, K. Nakao, M. Katsuki, S.-i. Taniguchi, T. Aoki, H. Sato, et al. Crosstalk between neovessels and mural cells directs the site-specific expression of MT1-MMP to endothelial tip cells J. Cell Sci., May 1, 2007; 120(9): 1607 - 1614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Piccard, P. E. Van den Steen, and G. Opdenakker Hemopexin domains as multifunctional liganding modules in matrix metalloproteinases and other proteins J. Leukoc. Biol., April 1, 2007; 81(4): 870 - 892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lee, C. M. Overall, C. A. McCulloch, and J. Sodek A Critical Role for the Membrane-type 1 Matrix Metalloproteinase in Collagen Phagocytosis Mol. Biol. Cell, November 1, 2006; 17(11): 4812 - 4826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Morrison and C. M. Overall TIMP Independence of Matrix Metalloproteinase (MMP)-2 Activation by Membrane Type 2 (MT2)-MMP Is Determined by Contributions of Both the MT2-MMP Catalytic and Hemopexin C Domains J. Biol. Chem., September 8, 2006; 281(36): 26528 - 26539. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |