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Papers In Press, published online ahead of print April 6, 2006
Pharmacology and Physiology, New Jersey Medical School, UMDNJ, Newark, NJ 07103
Corresponding Author: elsayewa{at}umdnj.edu
Previous work has shown that channels formed by both connexin26 (Cx26) and connexin32 (Cx32) (heteromeric Cx26/Cx32 hemichannels) are selectively permeable to cAMP and cGMP. To further investigate differential connexin channel permeability among second messengers, and the influence of connexin channel composition on the selectivity, the permeability of inositol phosphates with one to four phosphate groups through homomeric Cx26, homomeric Cx32 and heteromeric Cx26/Cx32 channels was examined. Connexin channels were purified from transfected HeLa cells and from rat, mouse and guinea pig livers, resulting in channels with a broad range of Cx26/Cx32 aggregate ratios. Permeability to inositol phosphates was assessed by flux through reconstituted channels. Surprisingly, myo-inositol and all inositol phosphates tested were permeable through homomeric Cx32 and homomeric Cx26 channels. Even more surprising, heteromeric Cx26/Cx32 channels showed striking differences in permeability among inositol phosphates with three or four phosphate groups, and among isomers of inositol triphosphate. Thus, heteromeric channels are selectively permeable among inositol phosphates whereas the corresponding homomeric channels are not. There was no discernable difference in the permeability of channels with similar Cx26/Cx32 ratios purified from native and heterologous sources. The molecular selectivity of heteromeric channels among three inositol triphosphates could not be accounted for by simple connexin isoform stoichiometry distributions, and therefore may depend on specific isoform radial arrangements within the hexameric channels. Dynamic regulation of channel composition in vivo may effectively and efficiently modulate intercellular signaling by inositol phosphates.
J. Biol. Chem, 10.1074/jbc.M600136200
Submitted on January 5, 2006
Accepted on April 6, 2006
Heteromeric, but not homomeric, connexin channels are selectively permeable to inositol phosphates
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