Potassium/proton antiport system of Escherichia coli

doi:10.1074/jbc.M600333200

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Papers In Press, published online ahead of print May 9, 2006
J. Biol. Chem, 10.1074/jbc.M600333200
Submitted on January 12, 2006
Revised on May 8, 2006
Accepted on May 9, 2006

Potassium/proton antiport system of Escherichia coli

Martha V. Radchenko, Kimihiro Tanaka, Rungaroon Waditee, Sawako Oshimi, Yasutomo Matsuzaki, Masahiro Fukuhara, Hiroshi Kobayashi, Teruhiro Takabe, and Tatsunosuke Nakamura

Microbiology, Niigata University of Pharmacy and Applied Life Sciences, Niigata 956-8603

Corresponding Author: tnak{at}niigata-pharm.ac.jp

The intracellular level of potassium (K+) in E. coli is regulated through multiple K+ transport systems. Recent data indicate that not all K+ extrusion system(s) have been identified (15). Here we report that the E. coli Na+/H+ antiporter ChaA functions as a K+ extrusion system. Cells expressing ChaA mediated K+ efflux against a K+ concentration gradient. E. coli strains lacking the chaA gene were unable to extrude K+ under conditions in which wild type cells extruded K+. The K+/H+ antiporter activity of ChaA was detected by using inverted membrane vesicles produced using a French press. Physiological growth studies indicated that E. coli uses ChaA to discard excessive K+, which is toxic for these cells. These results suggest that ChaA K+/H+ antiporter activity enables E. coli to adapt to K+ salinity stress and to maintain K+ homeostasis.

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