| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print June 12, 2006
Donald Danforth Plant Scienc Center, Saint Louis, MO 63132
Corresponding Author: jmarkham{at}danforthcenter.org
Sphingolipids are major components of the plasma membrane, tonoplast and other endomembranes of plant cells. Previous compositional analyses have focused only on individual sphingolipid classes because of the widely differing polarities of plant sphingolipids. Consequently, the total content of sphingolipid classes in plants has yet to be quantified. In addition, the major polar sphingolipid class in the model plant Arabidopsis thaliana has not been previously determined. In this report, we describe the separation and quantification of sphingolipid classes from A. thaliana leaves using hydrolysis of sphingolipids and HPLC analysis of o-phthaldialdehyde derivatives of the released long-chain bases to monitor the separation steps. An extraction solvent that contained substantial proportions of water was used to solubilized >95% of the sphingolipids from leaves. Neutral and charged sphingolipids were then partitioned by anion-exchange solid-phase extraction. HPLC analysis of the charged lipid fraction from A. thaliana revealed only one major anionic sphingolipid class which was identified by mass spectrometry as hexose-hexuronic-inositolphospho-ceramide. The neutral sphingolipids were predominantly composed of monohexosylceramide with lesser amounts of ceramides. Extraction and separation of sphingolipids from soybean and tomato showed that, like A. thaliana, the neutral sphingolipids consisted of ceramide and monohexosylceramides, however the major polar sphingolipid was found to be N-acetyl-hexosamine-hexuronic-inositol-phosphoceramide. In extracts from A. thaliana leaves, hexose-hexuronic-inositolphosphoceramides, monohexosylceramides and ceramides accounted for approximately 64%, 34% and 2% of the total sphingolipids, respectively, suggesting an important role for the anionic sphingolipids in plant membranes.
J. Biol. Chem, 10.1074/jbc.M604050200
Submitted on April 27, 2006
Revised on June 9, 2006
Accepted on June 12, 2006
Plant sphingolipids: separation and identification of major sphingolipid classes from leaves
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Chen, J. E. Markham, C. R. Dietrich, J. G. Jaworski, and E. B. Cahoon Sphingolipid Long-Chain Base Hydroxylation Is Important for Growth and Regulation of Sphingolipid Content and Composition in Arabidopsis PLANT CELL, July 1, 2008; 20(7): 1862 - 1878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Raffaele, F. Vailleau, A. Leger, J. Joubes, O. Miersch, C. Huard, E. Blee, S. Mongrand, F. Domergue, and D. Roby A MYB Transcription Factor Regulates Very-Long-Chain Fatty Acid Biosynthesis for Activation of the Hypersensitive Cell Death Response in Arabidopsis PLANT CELL, March 1, 2008; 20(3): 752 - 767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-M. Qin, C.-Y. Hu, Y. Pang, A. J. Kastaniotis, J. K. Hiltunen, and Y.-X. Zhu Saturated Very-Long-Chain Fatty Acids Promote Cotton Fiber and Arabidopsis Cell Elongation by Activating Ethylene Biosynthesis PLANT CELL, November 1, 2007; 19(11): 3692 - 3704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tsegaye, C. G. Richardson, J. E. Bravo, B. J. Mulcahy, D. V. Lynch, J. E. Markham, J. G. Jaworski, M. Chen, E. B. Cahoon, and T. M. Dunn Arabidopsis Mutants Lacking Long Chain Base Phosphate Lyase Are Fumonisin-sensitive and Accumulate Trihydroxy-18:1 Long Chain Base Phosphate J. Biol. Chem., September 21, 2007; 282(38): 28195 - 28206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Lefebvre, F. Furt, M.-A. Hartmann, L. V. Michaelson, J.-P. Carde, F. Sargueil-Boiron, M. Rossignol, J. A. Napier, J. Cullimore, J.-J. Bessoule, et al. Characterization of Lipid Rafts from Medicago truncatula Root Plasma Membranes: A Proteomic Study Reveals the Presence of a Raft-Associated Redox System Plant Physiology, May 1, 2007; 144(1): 402 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Laloi, A.-M. Perret, L. Chatre, S. Melser, C. Cantrel, M.-N. Vaultier, A. Zachowski, K. Bathany, J.-M. Schmitter, M. Vallet, et al. Insights into the Role of Specific Lipids in the Formation and Delivery of Lipid Microdomains to the Plasma Membrane of Plant Cells Plant Physiology, January 1, 2007; 143(1): 461 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Chen, G. Han, C. R. Dietrich, T. M. Dunn, and E. B. Cahoon The Essential Nature of Sphingolipids in Plants as Revealed by the Functional Identification and Characterization of the Arabidopsis LCB1 Subunit of Serine Palmitoyltransferase PLANT CELL, December 1, 2006; 18(12): 3576 - 3593. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
