JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on June 22, 2007 Originally published In Press as doi:10.1074/jbc.M700708200 on March 29, 2007
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
282/25/18141    most recent
M700708200v2
M700708200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Furland, N. E.
Right arrow Articles by Maldonado, M. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Furland, N. E.
Right arrow Articles by Maldonado, M. I.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print March 28, 2007
J. Biol. Chem, 10.1074/jbc.M700708200
Submitted on January 24, 2007
Accepted on March 28, 2007

Ceramides and sphingomyelins with high proportions of very long-chain polyunsaturated fatty acids in mammalian germ cells

Natalia Edith Furland, Samanta Romina Zanetti, Gerardo Martín Oresti, Eduardo Néstor Maldonado, and Marta Isabel Maldonado

Instituto de Investigaciones Bioquímicas Bahía Blanca, Bahía Blanca, Buenos Aires 8000

Corresponding Author: avelda{at}criba.edu.ar

Very long-chain polyunsaturated fatty acids (VLCPUFA) have previously been shown to be components of sphingomyelin (SM) of mam-malian testis and spermatozoa. Here we exam-ined the fatty acids of testicular ceramide (Cer) in comparison with those of SM in some mam-mals with a special focus on the rat testis. In bull, cat, dog, rabbit, mouse, and rat, VLCPUFA were found in both testicular lipids, Cer having a higher percentage of VLCPUFA than SM. Rat testis had the highest percentage of VLCPUFA in both lipids, the major ones being 28:4n-6 and 30:5n-6. VLCPUFA-containing SM and Cer occurred in cells lo-cated in the seminiferous tubules, where germ cells had a higher percentage of these species than Sertoli cells. Seminiferous tubule frac-tionation showed that SM and Cer of mito-chondria and lysosomes had mostly saturates and negligible VLCPUFA, the latter being im-portant in the SM and Cer of microsomes and other membrane fractions. VLCPUFA were absent from the SM and Cer of rat prepuberal testis, increased with the onset of spermato-genesis to account for nearly 15% and 40% of the total fatty acids of testicular SM and Cer, respectively, remained at those levels through-out the adult life of fertile rats, and tended to decrease at advanced ages. Four conditions that lead to selective death of germ cells in vivo, namely experimental cryptorchidism, post-ischemic reperfusion, focalized X-ray irradia-tion and treatments with the antineoplasic drug doxorubicin, caused the VLCPUFA to disap-pear from the testicular SM and Cer of adult fertile rats, showing that these lipids are specific traits of spermatogenic cells.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Rabionet, A. C. van der Spoel, C.-C. Chuang, B. von Tumpling-Radosta, M. Litjens, D. Bouwmeester, C. C. Hellbusch, C. Korner, H. Wiegandt, K. Gorgas, et al.
Male Germ Cells Require Polyenoic Sphingolipids with Complex Glycosylation for Completion of Meiosis: A LINK TO CERAMIDE SYNTHASE-3
J. Biol. Chem., May 9, 2008; 283(19): 13357 - 13369.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. M. Walden, R. Sandhoff, C.-C. Chuang, Y. Yildiz, T. D. Butters, R. A. Dwek, F. M. Platt, and A. C. van der Spoel
Accumulation of Glucosylceramide in Murine Testis, Caused by Inhibition of beta-Glucosidase 2: IMPLICATIONS FOR SPERMATOGENESIS
J. Biol. Chem., November 9, 2007; 282(45): 32655 - 32664.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. E. Furland, G. M. Oresti, S. S. Antollini, A. Venturino, E. N. Maldonado, and M. I. Aveldano
Very Long-chain Polyunsaturated Fatty Acids Are the Major Acyl Groups of Sphingomyelins and Ceramides in the Head of Mammalian Spermatozoa
J. Biol. Chem., June 22, 2007; 282(25): 18151 - 18161.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2007 by the American Society for Biochemistry and Molecular Biology.