| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 268, Issue 26, 19463-19470, 09, 1993
MS Mahendroo, CR Mendelson and ER Simpson
Estrogen biosynthesis is catalyzed by a microsomal enzyme, aromatase
cytochrome P450 (P450arom; the product of the CYP19 gene). The human CYP19
gene comprises nine coding exons, II-X. Additionally, tissue- specific
expression is determined by the use of tissue-specific promoters, which
give rise to P450arom transcripts with unique 5'- noncoding sequences. In
placenta, P450arom transcripts contain one of two 5'-untranslated exons,
I.1 or I.2, while ovarian transcripts instead contain sequence consistent
with the use of a promoter, PII, which is proximal to the start of
translation. To characterize transcripts present in adipose tissue and
adipose stromal cells (ASC) in culture, cDNA libraries were constructed by
the RACE (rapid amplification of cDNA ends) procedure. Four P450arom
transcripts with unique 5' termini were identified, leading to the
characterization of two unique 5'-untranslated exons of the CYP19 gene, I.3
and I.4. Whereas I.3-specific sequence is expressed in adipose tissue as
well as in ACS maintained under all culture conditions, I.4-specific
sequence is apparently present only in breast adipose tissue, and ACS
stimulated with glucocorticoids. On the other hand, PII-specific sequence
is present only in cells stimulated with cAMP analogues and is absent from
cells stimulated with glucocorticoids. We conclude that CYP19 gene
expression in human adipose tissue likely utilizes two novel promoters and,
furthermore, that alternative promoter usage in cultured ASC is a function
of the hormonal environment in which the cells are maintained.
Tissue-specific and hormonally controlled alternative promoters regulate aromatase cytochrome P450 gene expression in human adipose tissue
Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas 75235.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Kijima, J. Ye, C. Glackin, and S. Chen CCAAT/Enhancer Binding Protein {delta} Up-regulates Aromatase Promoters I.3/II in Breast Cancer Epithelial Cells Cancer Res., June 1, 2008; 68(11): 4455 - 4464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Bukulmez, D. B. Hardy, B. R. Carr, R. A. Word, and C. R. Mendelson Inflammatory Status Influences Aromatase and Steroid Receptor Expression in Endometriosis Endocrinology, March 1, 2008; 149(3): 1190 - 1204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Subbaramaiah, C. Hudis, S.-H. Chang, T. Hla, and A. J. Dannenberg EP2 and EP4 Receptors Regulate Aromatase Expression in Human Adipocytes and Breast Cancer Cells: EVIDENCE OF A BRCA1 AND p300 EXCHANGE J. Biol. Chem., February 8, 2008; 283(6): 3433 - 3444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Demura, R. M. Martin, M. Shozu, S. Sebastian, K. Takayama, W.-T. Hsu, R. A. Schultz, K. Neely, M. Bryant, B. B. Mendonca, et al. Regional rearrangements in chromosome 15q21 cause formation of cryptic promoters for the CYP19 (aromatase) gene Hum. Mol. Genet., November 1, 2007; 16(21): 2529 - 2541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Chen, S. Reierstad, Z. Lin, M. Lu, C. Brooks, N. Li, J. Innes, and S. E. Bulun Prostaglandin E2 Induces Breast Cancer Related Aromatase Promoters via Activation of p38 and c-Jun NH2-Terminal Kinase in Adipose Fibroblasts Cancer Res., September 15, 2007; 67(18): 8914 - 8922. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Sanderson The Steroid Hormone Biosynthesis Pathway as a Target for Endocrine-Disrupting Chemicals Toxicol. Sci., November 1, 2006; 94(1): 3 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lu, D. Chen, Z. Lin, S. Reierstad, A. M. Trauernicht, T. G. Boyer, and S. E. Bulun BRCA1 Negatively Regulates the Cancer-Associated Aromatase Promoters I.3 and II in Breast Adipose Fibroblasts and Malignant Epithelial Cells J. Clin. Endocrinol. Metab., November 1, 2006; 91(11): 4514 - 4519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Kijima, S. Phung, G. Hur, S.-L. Kwok, and S. Chen Grape Seed Extract Is an Aromatase Inhibitor and a Suppressor of Aromatase Expression Cancer Res., June 1, 2006; 66(11): 5960 - 5967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Subbaramaiah, L. R. Howe, E. R. Port, E. Brogi, J. Fishman, C. H. Liu, T. Hla, C. Hudis, and A. J. Dannenberg HER-2/neu Status Is a Determinant of Mammary Aromatase Activity In vivo: Evidence for a Cyclooxygenase-2-Dependent Mechanism. Cancer Res., May 15, 2006; 66(10): 5504 - 5511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Deb, J. Zhou, S. A. Amin, A. G. Imir, M. B. Yilmaz, Z. Lin, and S. E. Bulun A Novel Role of Sodium Butyrate in the Regulation of Cancer-associated Aromatase Promoters I.3 and II by Disrupting a Transcriptional Complex in Breast Adipose Fibroblasts J. Biol. Chem., February 3, 2006; 281(5): 2585 - 2597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Attar and S.E. Bulun Aromatase and other steroidogenic genes in endometriosis: translational aspects Hum. Reprod. Update, January 1, 2006; 12(1): 49 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Bouchard, H. Taniguchi, and R. S. Viger Protein Kinase A-Dependent Synergism between GATA Factors and the Nuclear Receptor, Liver Receptor Homolog-1, Regulates Human Aromatase (CYP19) PII Promoter Activity in Breast Cancer Cells Endocrinology, November 1, 2005; 146(11): 4905 - 4916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Bulun, Z. Lin, G. Imir, S. Amin, M. Demura, B. Yilmaz, R. Martin, H. Utsunomiya, S. Thung, B. Gurates, et al. Regulation of Aromatase Expression in Estrogen-Responsive Breast and Uterine Disease: From Bench to Treatment Pharmacol. Rev., September 1, 2005; 57(3): 359 - 383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. R. Simpson, M. Misso, K. N. Hewitt, R. A. Hill, W. C. Boon, M. E. Jones, A. Kovacic, J. Zhou, and C. D. Clyne Estrogen--the Good, the Bad, and the Unexpected Endocr. Rev., May 1, 2005; 26(3): 322 - 330. [Full Text] [PDF]
|
|
|
|
|
|
S. Deb, S. Amin, A. G. Imir, M. B. Yilmaz, T. Suzuki, H. Sasano, and S. E. Bulun Estrogen Regulates Expression of Tumor Necrosis Factor Receptors in Breast Adipose Fibroblasts J. Clin. Endocrinol. Metab., August 1, 2004; 89(8): 4018 - 4024. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Heim, O. Frank, G. Kampmann, N. Sochocky, T. Pennimpede, P. Fuchs, W. Hunziker, P. Weber, I. Martin, and I. Bendik The Phytoestrogen Genistein Enhances Osteogenesis and Represses Adipogenic Differentiation of Human Primary Bone Marrow Stromal Cells Endocrinology, February 1, 2004; 145(2): 848 - 859. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bourguiba, S. Chater, C. Delalande, M. Benahmed, and S. Carreau Regulation of Aromatase Gene Expression in Purified Germ Cells of Adult Male Rats: Effects of Transforming Growth Factor {beta}, Tumor Necrosis Factor {alpha}, and Cyclic Adenosine 3',5'-Monosphosphate Biol Reprod, August 1, 2003; 69(2): 592 - 601. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kinoshita and S. Chen Induction of Aromatase (CYP19) Expression in Breast Cancer Cells through a Nongenomic Action of Estrogen Receptor {alpha} Cancer Res., July 1, 2003; 63(13): 3546 - 3555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Richards and R. W. Brueggemeier Prostaglandin E2 Regulates Aromatase Activity and Expression in Human Adipose Stromal Cells via Two Distinct Receptor Subtypes J. Clin. Endocrinol. Metab., June 1, 2003; 88(6): 2810 - 2816. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Shozu, S. Sebastian, K. Takayama, W.-T. Hsu, R. A. Schultz, K. Neely, M. Bryant, and S. E. Bulun Estrogen Excess Associated with Novel Gain-of-Function Mutations Affecting the Aromatase Gene N. Engl. J. Med., May 8, 2003; 348(19): 1855 - 1865. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Catalano, V. Pezzi, A. Chimento, C. Giordano, A. Carpino, M. Young, M. J. McPhaul, and S. Ando Triiodothyronine Decreases the Activity of the Proximal Promoter (PII) of the Aromatase Gene in the Mouse Sertoli Cell Line, TM4 Mol. Endocrinol., May 1, 2003; 17(5): 923 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Rubin, J. H. Duong, C. D. Clyne, C. J. Speed, Y. Murata, C. Gong, and E. R. Simpson Ligands for the Peroxisomal Proliferator-Activated Receptor {gamma} and the Retinoid X Receptor Inhibit Aromatase Cytochrome P450 (CYP19) Expression Mediated by Promoter II in Human Breast Adipose Endocrinology, August 1, 2002; 143(8): 2863 - 2871. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Davies, L.-A. Martin, N. Sacks, and M. Dowsett Cyclooxygenase-2 (COX-2), aromatase and breast cancer: a possible role for COX-2 inhibitors in breast cancer chemoprevention Ann. Onc., May 1, 2002; 13(5): 669 - 678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Kado, J. Kitawaki, H. Obayashi, H. Ishihara, H. Koshiba, I. Kusuki, K. Tsukamoto, G. Hasegawa, N. Nakamura, T. Yoshikawa, et al. Association of the CYP17 gene and CYP19 gene polymorphisms with risk of endometriosis in Japanese women Hum. Reprod., April 1, 2002; 17(4): 897 - 902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. R. Simpson and M. Dowsett Aromatase and Its Inhibitors: Significance for Breast Cancer Therapy Recent Prog. Horm. Res., January 1, 2002; 57(1): 317 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Simpson and S. R. Davis Minireview: Aromatase and the Regulation of Estrogen Biosynthesis--Some New Perspectives Endocrinology, November 1, 2001; 142(11): 4589 - 4594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. O'Donnell, K. M. Robertson, M. E. Jones, and E. R. Simpson Estrogen and Spermatogenesis Endocr. Rev., June 1, 2001; 22(3): 289 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lanzino, S. Catalano, C. Genissel, S. Ando, S. Carreau, K. Hamra, and M. J. McPhaul Aromatase Messenger RNA Is Derived from the Proximal Promoter of the Aromatase Gene in Leydig, Sertoli, and Germ Cells of the Rat Testis Biol Reprod, May 1, 2001; 64(5): 1439 - 1443. [Abstract] [Full Text]
|
|
|
|
|
|
J. Zhou, B. Gurates, S. Yang, S. Sebastian, and S. E. Bulun Malignant Breast Epithelial Cells Stimulate Aromatase Expression via Promoter II in Human Adipose Fibroblasts: An Epithelial-Stromal Interaction in Breast Tumors Mediated by CCAAT/Enhancer Binding Protein {beta} Cancer Res., March 1, 2001; 61(5): 2328 - 2334. [Abstract] [Full Text]
|
|
|
|
|
|
D. Zhou, K. M. Quach, C. Yang, S. Y. Lee, B. Pohajdak, and S. Chen PNRC: A Proline-Rich Nuclear Receptor Coregulatory Protein That Modulates Transcriptional Activation of Multiple Nuclear Receptors Including Orphan Receptors SF1 (Steroidogenic Factor 1) and ERR{alpha}1 (Estrogen Related Receptor {alpha}-1) Mol. Endocrinol., July 1, 2000; 14(7): 986 - 998. [Abstract] [Full Text]
|
|
|
|
|
|
G. L. Rubin, Y. Zhao, A. M. Kalus, and E. R. Simpson Peroxisome Proliferator-activated Receptor {{gamma}} Ligands Inhibit Estrogen Biosynthesis in Human Breast Adipose Tissue: Possible Implications for Breast Cancer Therapy Cancer Res., March 1, 2000; 60(6): 1604 - 1608. [Abstract] [Full Text]
|
|
|
|
|
|
R. J. Pauley, S. J. Santner, L. R. Tait, R. K. Bright, and R. J. Santen Regulated CYP19 Aromatase Transcription in Breast Stromal Fibroblasts J. Clin. Endocrinol. Metab., February 1, 2000; 85(2): 837 - 846. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. S. Healey, A. M. Dunning, F. Durocher, D. Teare, P. D.P. Pharoah, R. N. Luben, D. F. Easton, and B. A.J. Ponder Polymorphisms in the human aromatase cytochrome P450 gene (CYP19) and breast cancer risk Carcinogenesis, February 1, 2000; 21(2): 189 - 193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Boerboom, A. Kerban, and J. Sirois Dual Regulation of Promoter II- and Promoter 1f-Derived Cytochrome P450 Aromatase Transcripts in Equine Granulosa Cells during Human Chorionic Gonadotropin-Induced Ovulation: A Novel Model for the Study of Aromatase Promoter Switching Endocrinology, September 1, 1999; 140(9): 4133 - 4141. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Kamat, K. H. Graves, M. E. Smith, J. A. Richardson, and C. R. Mendelson A 500-bp region, approx 40 kb upstream of the human CYP19 () gene, mediates placenta-specific expression in transgenic mice PNAS, April 13, 1999; 96(8): 4575 - 4580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Zeitoun, K. Takayama, M. D. Michael, and S. E. Bulun Stimulation of Aromatase P450 Promoter (II) Activity in Endometriosis and Its Inhibition in Endometrium Are Regulated by Competitive Binding of Steroidogenic Factor-1 and Chicken Ovalbumin Upstream Promoter Transcription Factor to the Same cis-Acting Element Mol. Endocrinol., February 1, 1999; 13(2): 239 - 253. [Abstract] [Full Text]
|
|
|
|
|
|
A. Kamat, J. L. Alcorn, C. Kunczt, and C. R. Mendelson Characterization of the Regulatory Regions of the Human Aromatase (P450arom) Gene Involved in Placenta-Specific Expression Mol. Endocrinol., November 1, 1998; 12(11): 1764 - 1777. [Abstract] [Full Text]
|
|
|
|
|
|
L. Dalla Valle, A. Ramina, S. Vianello, A. Fassina, P. Belvedere, and L. Colombo Potential for Estrogen Synthesis and Action in Human Normal and Neoplastic Thyroid Tissues J. Clin. Endocrinol. Metab., October 1, 1998; 83(10): 3702 - 3709. [Abstract] [Full Text]
|
|
|
|
|
|
C. A. Stratakis, A. Vottero, A. Brodie, L. S. Kirschner, D. DeAtkine, Q. Lu, W. Yue, C. S. Mitsiades, A. W. Flor, and G. P. Chrousos The Aromatase Excess Syndrome Is Associated with Feminization of Both Sexes and Autosomal Dominant Transmission of Aberrant P450 Aromatase Gene Transcription J. Clin. Endocrinol. Metab., April 1, 1998; 83(4): 1348 - 1357. [Abstract] [Full Text]
|
|
|
|
|
|
M. Shozu, Y. Zhao, S. E. Bulun, and E. R. Simpson Multiple Splicing Events Involved in Regulation of Human Aromatase Expression by a Novel Promoter, I.6 Endocrinology, April 1, 1998; 139(4): 1610 - 1617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sun, Y. Zhao, D. J. Mangelsdorf, and E. R. Simpson Characterization of a Region Upstream of Exon I.1 of the Human CYP19 (Aromatase) Gene That Mediates Regulation by Retinoids in Human Choriocarcinoma Cells Endocrinology, April 1, 1998; 139(4): 1684 - 1691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shozu, Y. Zhao, and E. R. Simpson Estrogen Biosynthesis in THP1 Cells Is Regulated by Promoter Switching of the Aromatase (CYP19) Gene Endocrinology, December 1, 1997; 138(12): 5125 - 5135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Hinshelwood, M. D. Michael, and E. R. Simpson The 5'-Flanking Region of the Ovarian Promoter of the Bovine CYP19 Gene Contains a Deletion in a Cyclic Adenosine 3',5'-Monophosphate-Like Responsive Sequence Endocrinology, September 1, 1997; 138(9): 3704 - 3710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Furbass, C. Kalbe, and J. Vanselow Tissue-Specific Expression of the Bovine Aromatase-Encoding Gene Uses Multiple Transcriptional Start Sites and Alternative First Exons Endocrinology, July 1, 1997; 138(7): 2813 - 2819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Noble, K. Takayama, K. M. Zeitoun, J. M. Putman, D. A. Johns, M. M. Hinshelwood, V. R. Agarwal, Y. Zhao, B. R. Carr, and S. E. Bulun Prostaglandin E2 Stimulates Aromatase Expression in Endometriosis-Derived Stromal Cells J. Clin. Endocrinol. Metab., February 1, 1997; 82(2): 600 - 606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. R. Agarwal, C. I. Ashanullah, E. R. Simpson, and S. E. Bulun Alternatively Spliced Transcripts of the Aromatase Cytochrome P450 (CYP19) Gene in Adipose Tissue of Women J. Clin. Endocrinol. Metab., January 1, 1997; 82(1): 70 - 74. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zhou, P. Clarke, J. Wang, and S. Chen Identification of a Promoter That Controls Aromatase Expression in Human Breast Cancer and Adipose Stromal Cells J. Biol. Chem., June 21, 1996; 271(25): 15194 - 15202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Hu, L. Zhuang, and M. L. Dufau Multiple and Tissue-specific Promoter Control of Gonadal and Non-gonadal Prolactin Receptor Gene Expression J. Biol. Chem., April 26, 1996; 271(17): 10242 - 10246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhao, J. E. Nichols, S. E. Bulun, C. R. Mendelson, and E. R. Simpson Aromatase P450 Gene Expression in Human Adipose Tissue J. Biol. Chem., July 7, 1995; 270(27): 16449 - 16457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Michael, M. W. Kilgore, K.-i. Morohashi, and E. R. Simpson Ad4BP/SF-1 Regulates Cyclic AMP-induced Transcription from the Proximal Promoter (PII) of the Human Aromatase P450 (CYP19) Gene in the Ovary J. Biol. Chem., June 2, 1995; 270(22): 13561 - 13566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Nichols, S. E. Bulun, and E. R. Simpson Effects of Conditioned Medium from Different Cultured Cell Types on Aromatase Expression in Adipose Stromal Cells Reproductive Sciences, January 1, 1995; 2(1): 45 - 50. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
