J Biol Chem, Vol. 274, Issue 16, 10677-10680, April 16, 1999

COMMUNICATION
Identification of NSF as a -Arrestin1-binding Protein
IMPLICATIONS FOR ₂-ADRENERGIC RECEPTOR REGULATION^*

Patricia H. McDonald, Nicole L. Cote, Fang-Tsyr Lin, Richard T. Premont, Julie A. Pitcher, and Robert J. Lefkowitz

From the Howard Hughes Medical Institute and the Departments of Medicine (Cardiology) and Biochemistry, Duke University Medical Center, Durham, North Carolina 27710

	ABSTRACT

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS AND DISCUSSION REFERENCES

Previous studies have demonstrated that -arrestin1 serves to target G protein-coupled receptors for internalization via clathrin-coated pits and that its endocytic function is regulated by dephosphorylation at the plasma membrane. Using the yeast two-hybrid system, we have identified a novel -arrestin1-binding protein, NSF (N-ethylmaleimide-sensitive fusion protein), an ATPase essential for many intracellular transport reactions. We demonstrate that purified recombinant -arrestin1 and NSF interact in vitro and that these proteins can be coimmunoprecipitated from cells. -Arrestin1-NSF complex formation exhibits a conformational dependence with -arrestin1 preferentially interacting with the ATP bound form of NSF. In contrast to the -arrestin1-clathrin interaction, however, the phosphorylation state of -arrestin1 does not affect NSF binding. Functionally, overexpression of NSF in HEK 293 cells significantly enhances agonist-mediated ₂-adrenergic receptor (₂-AR) internalization. Furthermore, when coexpressed with a -arrestin1 mutant (arr1S412D) that mimics a constitutively phosphorylated form of -arrestin1 and that acts as a dominant negative with regards to ₂-AR internalization, NSF rescues the arr1S412D-mediated inhibition of ₂-AR internalization. The demonstration of -arrestin1-NSF complex formation and the functional consequences of NSF overexpression suggest a hitherto unappreciated role for NSF in facilitating clathrin coat-mediated G protein-coupled receptor internalization.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS AND DISCUSSION REFERENCES

Many G protein-coupled receptors (GPCRs),¹ such as the ₂-adrenergic receptor (₂-AR), utilize classical clathrin-coated vesicle pathways for internalization. Several lines of evidence indicate that phosphorylation of the receptor by a G protein-coupled receptor kinase (GRK) followed by -arrestin binding are crucial steps in this process (reviewed in Refs. 1-3). The role of GRK-mediated receptor phosphorylation in the internalization of receptors is to facilitate the binding of -arrestins. -Arrestins serve a dual role in the regulation of GPCRs. First, they mediate rapid desensitization by binding to GRK-phosphorylated receptors, sterically interdicting signaling to the G protein (4). Second, they target receptors for internalization via clathrin-coated pits, allowing subsequent dephosphorylation and resensitization of the receptors (5). Thus, a mutated form of the ₂-AR (Y326A) that is a poor substrate for the GRKs fails to undergo agonist-dependent internalization. However, receptor internalization can be rescued by overexpressing GRKs (6). Similarly, overexpressing -arrestin1 with the Y326A mutant also rescues receptor internalization (7). Further confirming the essential role of -arrestins in the internalization process is the finding that dominant negative forms of -arrestin1, namely V53D (8) and arr1S412D (5), dramatically impair agonist-stimulated receptor sequestration.

It has been previously demonstrated that -arrestin1 and 2 bind directly and stoichiometrically to clathrin (9) and that the function of -arrestin1 in GPCR internalization is regulated by phosphorylation/dephosphorylation of the -arrestin1 molecule (5). Cytoplasmic -arrestin1 is constitutively phosphorylated on a carboxyl-terminal serine (Ser-412) and is recruited to the plasma membrane upon agonist stimulation of the receptor, where it becomes rapidly dephosphorylated. Dephosphorylation of -arrestin1 is required for clathrin binding and the subsequent targeting of receptors to clathrin-coated pits but not for receptor binding and receptor desensitization. It is interesting to note that other members of the arrestin family (visual arrestin, -arrestin2, and splice variants) do not possess the Ser-412 residue and are therefore not subject to the same regulation as -arrestin1. Whether the ability of -arrestin1 to promote GPCR internalization arises solely as a consequence of its ability to bind clathrin or whether additional -arrestin1-binding proteins are involved in this process remains to be determined. In an attempt to further define the role of -arrestin1 in the regulation of GPCR function, we used the yeast two-hybrid system to screen for novel -arrestin1-binding proteins.

	EXPERIMENTAL PROCEDURES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS AND DISCUSSION REFERENCES

Yeast Two-hybrid Screen-- The full cDNA of rat -arrestin1 was subcloned into the yeast expression vector pGBT9 using EcoRI and BamHI. Following amplification in Escherichia coli, the pGBT9 -arrestin1 plasmid was transformed into yeast strain HF7c, and a yeast two-hybrid screen was carried out essentially according to the CLONTECH MATCHMAKER protocol using a GAL4 activation domain fusion library in pGAD10 (MATCHMAKER rat whole brain cDNA library, CLONTECH). Interacting proteins were isolated by growth selection on Trp, His, and Leu dropout plates and subsequently by filter -galactosidase assays. Isolated library clones were analyzed by DNA sequencing using an ABI automated DNA sequencer (Howard Hughes Nucleic Acid Facility, Duke University).

Cell Culture and Transfection-- The plasmids of interest were transfected into either HEK 293 cells (for sequestration assays) or COS cells (cellular coimmunoprecipitation) using LipofectAMINE according to the manufacturer's instructions (Life Technologies, Inc). To determine the association of -arrestin1 and NSF, COS cells were cotransfected with either Flag-tagged -arrestin1 (or Flag-tagged arr1S412A and arr1S412D) and/or His₆-NSF-c-Myc, pcDNA3 empty vector was used as a control. Cells were harvested in lysis buffer (LB; 50 mM HEPES, pH 7.4, 0.5% Nonidet P-40, 250 mM NaCl, 10% glycerol, 2 mM EDTA, 0.5 mM ATP, 1 mM dithiothreitol, 1 µM phenylmethylsulfonyl fluoride 25 µg/ml apoprotin, and 1 µM leupeptin). The -arrestins were immunoprecipitated using a polyclonal M2 antibody specific to the Flag epitope. The proteins were resolved by SDS-PAGE. Western blot analysis was performed using a monoclonal antibody specific for NSF (6E6). NSF was visualized using either ECL or quantitated using alkaline phosphatase-conjugated secondary antibody (Amersham) followed by phosphoimager analysis. The nitrocellulose was stripped of immunoglobulin, and -arrestin1 was visualized or quantitated using an polyclonal antibody specific for -arrestin1 (19) to confirm equal expression of the -arrestins.

Determination of Nucleotide Dependence of the -Arrestin1-NSF Interaction-- Cellular coimmunoprecipitations were carried out as described above. COS-1 cells were cotransfected with plasmids encoding either empty vector, Flag--arrestin1, NSF, or Flag--arrestin1 plus NSF. Flag--arrestin1 was immunoprecipitated using an M2 monoclonal antibody specific for the Flag epitope. Immunoprecipitates were washed three times with LB buffer, then equally divided, and transferred to fresh tubes. One-half of each immunoprecipitate was given a final wash with LB buffer containing 0.5 mM ATP and 2 mM EDTA (ATP/EDTA), and the other half was washed with LB buffer containing 2 mM EDTA plus 10 mM MgCl₂ (ATP/Mg²⁺) to allow ATP hydrolysis on the immunoprecipitated NSF. Immunoprecipitates were resolved by SDS-PAGE and transferred to nitrocellulose membranes. NSF was visualized using the 6E6 monoclonal antibody and quantitated by phosphoimager analysis. Immunoblots were stripped of IgG and reprobed for -arrestin1 using an antibody specific to -arrestin1, such that the amount of NSF immunoprecipitated under nonhydrolyzable versus hydrolyzable conditions could be normalized to the amount of -arrestin1 immunoprecipitated.

₂-AR Sequestration as Determined by Flow Cytometry-- Agonist-induced ₂-AR internalization was determined by immunofluorescence flow cytometry analysis as has been described previously (31). HEK 293 cells were transiently transfected with the plasmid encoding a Flag-tagged ₂-AR and either a pcDNA3 control vector, a -arrestin1 expression plasmid (-arrestin1 or arr1S412D), an NSF expression vector, or NSF with arr1S412D. Receptor fluorescence was defined as signal above the fluorescence of untransfected cells. Sequestration is defined as the fraction of total cell surface receptors that are removed from the plasma membrane (and thus are not accessible to antibodies added to the cells) following agonist treatment.

	RESULTS AND DISCUSSION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS AND DISCUSSION REFERENCES

The Yeast Two-hybrid System Identifies NSF as a -Arrestin1-binding Protein-- To identify novel proteins that interact with -arrestin1 we screened a rat brain cDNA library using a full-length -arrestin1 cDNA as bait in the yeast two-hybrid system screen (10). Several clones positive for His cell growth and -galactosidase expression were isolated including a cDNA clone encoding a portion of the amino-terminal region of NSF (N-ethylmaleimide-sensitive fusion protein (NSF) (Fig. 1). This clone was found to require the Gal4 DNA-binding domain--arrestin1 fusion for activity. NSF is an ATPase whose hydrolytic activity has previously been demonstrated to play an essential role in intracellular membrane trafficking (11). Through its interaction with soluble NSF attachment proteins (SNAPs), NSF binds to a group of membrane receptors referred to as SNAP receptors (SNAREs) to form a complex known as the "20 S particle" (12). The ATPase activity of NSF disassembles the 20 S complex. NSF forms a homohexameric protein complex (13, 14) wherein each subunit contains three primary domains: an amino-terminal domain (N-domain) required for SNAP/SNARE binding and two ATPase domains, referred to as D1 and D2, required for fusion complex disassembly and hexamer formation respectively (Fig. 1) (15, 16). The region of NSF found to bind -arrestin1 lies within the N-domain, identified as the binding domain for other proteins known to interact with NSF (16-18).

View larger version (30K): [in this window] [in a new window]   Fig. 1.   Partial amino acid sequence of rat NSF. The amino acid sequence of the yeast two-hybrid cDNA clone isolated as a potential binding partner for -arrestin1 and its location within the NSF molecule is indicated. Each NSF subunit comprises three domains, the N-domain, essential for SNAP/SNARE binding, and two ATP-binding domains (D1 and D2), required for fusion complex disassembly and hexamer formation, respectively.

-Arrestin1 Binds NSF in Vitro and in Intact Cells-- Because the yeast two-hybrid system can detect weak interactions that may not occur under physiological conditions, we sought to confirm the yeast interaction data both in vitro and in a mammalian expression system. Recombinant -arrestin1 and recombinant NSF (both purified from E. coli) were incubated in the presence of ATP/EDTA (nonhydrolyzable conditions). -Arrestin1 was immunoprecipitated using a polyclonal antibody directed against -arrestin1 (19). The immunoprecipitates were subsequently probed for NSF using a monoclonal antibody directed against this protein (Fig. 2A, upper panel). A similar experiment was performed whereby NSF was immunoprecipitated and the immunoprecipitates were immunoblotted for -arrestin1 (Fig. 2A, lower panel). -Arrestin1 and NSF formed a complex in vitro in a manner that was dependent upon the concentration of the two protein components.

View larger version (17K): [in this window] [in a new window]   Fig. 2.   -Arrestin1 binds NSF in vitro and in intact cells. A, NSF (50 pmol) was incubated with increasing concentrations of -arrestin1 (0-50 pmol) in the presence of ATP/EDTA. -Arrestin1 was immunoprecipitated (IP) using a -arrestin1-specific polyclonal antibody. The immunoprecipitates were resolved by SDS-PAGE and immunoblotted (IB) for NSF using a monoclonal antibody specific for NSF. The upper panel shows that as increasing amounts of -arrestin1 are immunoprecipitated, increasing amounts of NSF can be visualized. Similarly, if -arrestin1 (50 pmol) is incubated with increasing amounts of NSF (0-50 pmol) and NSF is immunoprecipitated, increasing amounts of -arrestin1 are recovered (lower panel). B, when NSF and Flag-tagged -arrestin1 (wild type, arr1S412A, or arr1S412D) are coexpressed in COS cells and the -arrestins immunoprecipitated using an antibody specific to the Flag epitope, equal amounts of NSF are coimmunoprecipitated. Results are representative of four independent experiments.

To determine whether NSF and -arrestin1 associate in intact cells, a cDNA encoding NSF was cotransfected into COS cells with cDNA encoding Flag-tagged -arrestin1. The association of NSF with -arrestin1 was examined by immunoprecipitating -arrestin1 and analyzing the isolated immunoprecipitates for NSF content. As shown in Fig. 2B, in the absence of NSF overexpression, a complex of -arrestin1 and endogenous cellular NSF is detected (Fig. 2B, compare no arr1 with arr1 only). Moreover, overexpression of NSF increases the amount of -arrestin1-NSF complex immunoprecipitated (Fig. 2B, compare arr1 only and arr1 + NSF). These results demonstrate that -arrestin1 and NSF interact in cells. Coupled with the data showing an association between these proteins in vitro, this suggests that the -arrestin1-NSF interaction may be of physiological relevance.

It has previously been shown that the phosphorylation state of -arrestin1 regulates its endocytic function; the dephosphorylated form of -arrestin1 preferentially binds to clathrin and promotes GPCR internalization (5). The binding of -arrestin1 to the ₂-AR, however, is independent of its phosphorylation state (5). Therefore, we investigated the effect of -arrestin1 phosphorylation on NSF binding. Two -arrestin1 phosphorylation mutants, where Ser-412 has been replaced with either alanine (arr1S412A) or aspartate (arr1S412D) have been described previously (5). The arr1S412A mutant has been shown to simulate the dephosphorylated form of -arrestin1, whereas the arr1S412D mutant has been shown to mimic its phosphorylated form. The association of NSF with wild type -arrestin1, arr1S412A, or arr1S412D was comparable (Fig. 2B). The observation that -arrestin1-NSF complex formation is not dependent on the phosphorylation state of -arrestin1 suggests that NSF could potentially interact with -arrestin1 either in the cytosol (phosphorylated -arrestin1) and/or at the plasma membrane (dephosphorylated -arrestin1). -Arrestin1 has been shown to translocate from the cytosol to the plasma membrane and colocalize with clathrin-coated vesicles following agonist activation of the ₂-AR (8). Thus, interaction of -arrestin1 with NSF may serve to target NSF to the clathrin-coated vesicles following GPCR activation.

-Arrestin1-NSF Complex Formation Is ATP-dependent-- The association of NSF with its previously identified binding partners (SNAPs, SNAREs, and more recently the GluR2 subunit of the AMPA receptor) (17, 18) exhibits an ATP dependence. NSF has been shown to bind stably to SNARE complexes and does not dissociate from them when locked in the ATP state (13). To determine whether -arrestin1 binding to NSF is also modulated by the nucleotide status of NSF, immunoprecipitations from cells were performed either in the presence of ATP/EDTA or ATP/Mg²⁺ (ATP nonhydrolyzable and hydrolyzable conditions, respectively). As seen in Fig. 3. NSF preferentially coimmunoprecipitated with -arrestin1 in the presence of ATP/EDTA. The binding of NSF and -arrestin1 was significantly impaired under conditions in which ATP is hydrolyzed (ATP/Mg²⁺). Notably, a mutant NSF capable of binding but not hydrolyzing ATP (E329Q; described in Ref. 15) showed no significant difference in -arrestin1 binding in the presence of ATP/EDTA or ATP/Mg²⁺ (data not shown).

View larger version (42K): [in this window] [in a new window]   Fig. 3.   The -arrestin1-NSF complex formation is ATP-dependent. COS cells were transiently transfected with plasmids encoding Flag-tagged -arrestin1 (5 µg) or NSF (5 µg) as controls or Flag-tagged -arrestin1 and NSF (5 µg of each). Cells were harvested in lysis buffer (containing either 0.5 mM ATP/2 mM EDTA or 0.5 mM ATP/4 mM MgCl2) 48 h post-transfection. -Arrestin1 was immunoprecipitated using an antibody specific to the Flag epitope. The precipitates were resolved by SDS-PAGE and immunoblotted for NSF. The amount of NSF bound was normalized to the amount of -arrestin1 immunoprecipitated. Results represent the means ± S.E. for seven independent experiments.

Overexpression of NSF Potentiates ₂-AR Internalization-- -Arrestin1 has been shown to promote internalization (also referred to as sequestration) of agonist-occupied ₂-AR (7). If the -arrestin1-NSF interaction is important in regulating GPCR function, then given the well characterized role of NSF in vesicle trafficking, overexpression of NSF might also regulate ₂-AR internalization. Thus, HEK 293 cells were transfected with Flag-tagged ₂-AR alone or with wild type -arrestin1, arr1S412D, NSF, or NSF and arr1S412D. Consistent with previously published results (20), overexpression of ₂-AR with -arrestin1 in HEK 293 cells results in a modest enhancement of ₂-AR sequestration, and overexpression of the arr1S412D (5) mutant results in a reduction of ₂-AR sequestration compared with cells transfected with ₂-AR only (Fig. 4). Dramatically, NSF overexpression enhances ₂-AR sequestration approximately 2-fold. Furthermore, coexpression of NSF and the arr1S412D mutant in HEK 293 cells appears to rescue the dominant negative effect of arr1S412D, such that sequestration was found to be comparable with that in cells expressing ₂-AR only (Fig. 4). Coexpression of wild type -arrestin1 with NSF did not further enhance NSF-stimulated ₂-AR sequestration (data not shown).

View larger version (45K): [in this window] [in a new window]   Fig. 4.   Overexpression of NSF potentiates 2-AR internalization. Plasmid DNA encoding Flag tagged 2-ARs (1 µg) was transiently transfected into HEK 293 cells together with 10 µg of empty vector pCMV5 (control) or -arrestin1 and pCMV5 (5 µg of each) or arr1S412D and pCMV5 (5 µg each) or NSF and pCMV5 (5 µg of each) or arr1S412D and NSF (5 µg each). Sequestration was determined by flow cytometry analysis as described previously (27). Receptor sequestration is expressed as the percentage of loss of cell surface immunofluorescence following a 20-min exposure to 10 µM isoproterenol at 37 °C. Results are the means ± S.E. of three to five independent experiments.

NSF exists in both cytosolic and membrane-associated forms and was originally purified on the basis of its ability to complement in vitro intra-Golgi transport reactions that had been blocked by treatment with N-ethylmaleimide (21). NSF has a general role in vesicular transport and functions at most if not all vesicular transport events by virtue of its action on SNARE proteins. SNARE proteins have been classified into two families known as v-SNAREs (vesicle membrane receptor) and t-SNAREs (target membrane receptor) (12). The cognate pairing of a v-SNARE with a t-SNARE leads to the formation of a docking complex for membrane fusion to occur. The formation of this complex, termed the 20 S particle, is ATP-dependent. ATP hydrolysis by NSF disassembles the 20 S complex releasing the v-SNARE and t-SNAREs, thereby recycling these components for future fusion events (reviewed in Ref. 11). The observation that the neuronal SNAREs, syntaxin (v-SNARE) and synaptobrevin (t-SNARE), no longer interact with NSF following one round of ATP hydrolysis indicates that some structural modification occurs in one or both of these NSF-binding proteins (22). Taken together, these observations suggest that NSF promotes conformational changes in proteins with which it interacts in an ATP-dependent manner.

That the function of NSF is not restricted to vesicular transport is suggested by the observation that NSF binds to the AMPA receptor (17, 18, 23). AMPA receptors are a class of ionotropic glutamate receptors that mediate fast synaptic transmission and are composed of subunits GluR1-GluR4. Notably, NSF can bind directly to the GluR2 subunit of the AMPA receptor, providing the first example of an NSF cell surface receptor interaction (17, 18, 23). NSF and GluR2 exist in a complex in brain extracts not only with each other but also with SNAPs (18, 23). The GluR2-SNAP-NSF complex resembles the SNARE-SNAP-NSF complex in being disassembled by ATP hydrolysis (18). However, in contrast to the classical 20 S particle, NSF can bind directly to GluR2 independently of SNAPs. The binding site for NSF maps to a short region of the cytoplasmic tail of GluR2 distinct from the very carboxyl-terminal domain that interacts with the PDZ protein, GRIP (24). GRIP is believed to serve as a molecular scaffold on which a large complex of proteins is constructed, including AMPA receptors. It has been suggested that ATP hydrolysis by NSF may disassemble AMPA receptor interaction with GRIP leading to mobilization, insertion or internalization of the receptor (reviewed in Ref. 25).

Identification of an interaction between -arrestin1 and NSF brings together two proteins previously found to be associated with clathrin-coated vesicles. -Arrestin1 has been shown to interact with clathrin and target receptors to clathrin-coated pits for internalization (9). NSF has been shown to be associated with membranes derived from clathrin-coated vesicles (26). Purified clathrin-coated vesicles not only contain significant amounts of NSF but are enriched in the ability to support formation of 20 S fusion complexes. The current model for ₂-AR internalization requires agonist-activated receptor to recruit -arrestin to the plasma membrane, where it binds receptor. Following receptor binding, -arrestin1 targets the receptor to clathrin and promotes receptor internalization.

By analogy with the many proposed roles of NSF in vesicular transport, one can envisage several possible functions of NSF binding to -arrestin1 that might involve the NSF-driven association/disassociation of -arrestin1 with/from receptor and/or other components of the clathrin-coated vesicle. One potential role for the interaction of NSF with -arrestin1 may be to alter the conformation of -arrestin1, thereby regulating the interaction of this protein with other components of the endocytic pathway. One -arrestin1-binding partner whose interaction with -arrestin1 could potentially be facilitated by NSF is clathrin. This may provide a potential explanation for the observation that overexpression of NSF is able to rescue the dominant negative effect of the arr1S412D mutant. arr1S412D is believed to behave as a dominant negative with regards to ₂-AR internalization due to a reduced ability to bind clathrin (5). Because NSF binds equally well to arr1S412D and wild type -arrestin1, NSF could potentially induce conformational changes in arr1S412D, increasing its affinity for clathrin, thus allowing internalization to proceed. Whether or not NSF has effects on regulating the interaction of -arrestin with clathrin (and potentially other coat components) remains to be determined. A possible role of NSF in ₂-AR internalization by mediating vesicle fusion during endocytosis is presently unclear, because any potential SNAREs for this process have yet to be identified.

An alternative role for the NSF--arrestin1 interaction, by analogy to the proposed role of NSF in the AMPA-GRIP interaction, is that NSF binding to -arrestin1 might involve the NSF-driven dissociation of the ₂-AR--arrestin1 complex from elements of the cytoskeleton. NSF ATP hydrolysis might possibly release the receptor from some constraining influence, leading to mobilization and internalization. A detailed investigation of the function of -arrestin1-NSF complex formation may reveal hitherto unsuspected roles for NSF in clathrin coat-mediated endocytosis.

	ACKNOWLEDGEMENTS

We thank Drs. Randy Hall and Thomas Sollner for helpful discussion; Dr. James Rothman for providing purified NSF, NSF antibody (6E6), plasmids encoding His₆-NSF-c-Myc, and His₆-NSF(E329Q)-c-Myc; and W. Carl Stone for providing purified -arrestin1. We also thank Donna Addison and Mary Holben for excellent secretarial assistance.

	FOOTNOTES

^* This work is supported in part by National Institutes of Health Grant HL16037 (to R. J. L.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Investigator with the Howard Hughes Medical Institute. To whom correspondence should be addressed: Howard Hughes Medical Inst., Depts. of Medicine and Biochemistry, Duke University Medical Center, Box 3821, Durham, NC 27710. Tel.: 919-684-2974; Fax: 919-684-8875; E-mail: lefko001{at}mc.duke.edu.

	ABBREVIATIONS

The abbreviations used are: GPCR, G protein-coupled receptor; ₂-AR, ₂-adrenergic receptor; GRK, GPCR kinase; PAGE, polyacrylamide gel electrophoresis; PBS, phosphate-buffered saline; SNAP, soluble NSF attachment protein; SNARE, SNAP receptor; N-domain, amino-terminal domain.

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