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J Biol Chem, Vol. 274, Issue 27, 19246-19253, July 2, 1999


Transient Effect of Platelet-derived Growth Factor on GLUT4 Translocation in 3T3-L1 Adipocytes*

Lihong Wang, Hideki Hayashi, and Yousuke EbinaDagger

From the Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan

    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

We earlier developed a novel method to detect translocation of the glucose transporter (GLUT) directly and simply using c-MYC epitope-tagged GLUT (GLUTMYC). To define the effect of platelet-derived growth factor (PDGF) on glucose transport in 3T3-L1 adipocytes, we investigated the PDGF- and insulin-induced glucose uptake, translocation of glucose transporters, and phosphatidylinositol (PI) 3-kinase activity in 3T3-L1, 3T3-L1GLUT4MYC, and 3T3-L1GLUT1MYC adipocytes. Insulin and PDGF stimulated glucose uptake by 9-10- and 5.5-6.5-fold, respectively, in both 3T3-L1 and 3T3-L1GLUT4MYC adipocytes. Exogenous GLUT4MYC expression led to enhanced PDGF-induced glucose transport. In 3T3-L1GLUT4MYC adipocytes, insulin and PDGF induced an 8- and 5-fold increase in GLUT4MYC translocation, respectively, determined in a cell-surface anti-c-MYC antibody binding assay. This PDGF-induced GLUT4MYC translocation was further demonstrated with fluorescent detection. In contrast, PDGF stimulated a 2-fold increase of GLUT1MYC translocation and 2.5-fold increase of glucose uptake in 3T3-L1GLUT1MYC adipocytes. The PDGF-induced GLUT4MYC translocation, glucose uptake, and PI 3-kinase activity were maximal (100%) at 5-10 min and thereafter rapidly declined to 40, 30, and 12%, respectively, within 60 min, a time when effects of insulin were maximal. Wortmannin (0.1 µM) abolished PDGF-induced GLUT4MYC translocation and glucose uptake in 3T3-L1GLUT4MYC adipocytes. These results suggest that PDGF can transiently trigger the translocation of GLUT4 and stimulate glucose uptake by translocation of both GLUT4 and GLUT1 in a PI 3-kinase-dependent signaling pathway in 3T3-L1 adipocytes.

    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

The insulin signaling pathway mediating the glucose transport is not fully understood. Translocation of GLUT41 from an intracellular pool to the plasma membrane is thought to be a major mechanism of glucose uptake in response to insulin in insulin-sensitive tissues (1-3). We (4) and others (5-8) found that PI 3-kinase activation is essential for insulin-stimulated glucose uptake. Platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) activate many of the same signaling cascades as does insulin. One of the most prominent shared pathways is phosphatidylinositol 3-kinase. Therefore, the question arose as to whether PDGF or EGF would trigger GLUT4 translocation by the activation of PI 3-kinase. We developed a sensitive immunological method that can detect c-MYC epitope-tagged GLUT4 (GLUT4MYC) on the cell surface, directly and quantitatively (9). By using this method, we have found that PDGF and EGF did trigger the GLUT4 translocation to the plasma membrane in CHO and 3T3-L1 adipocytes by a signaling pathway involving phosphatidylinositol 3-kinase (PI 3-kinase, p85/p110 heterodimer type) (10, 11). We considered that PDGF and EGF as well as insulin may have latent potential to trigger GLUT4 translocation by activation of PI 3-kinase in cultured cells. PDGF- or EGF-triggered GLUT4 translocation has been reported by other research groups (12, 13). However, there is a great deal of debate on the effect of PDGF on glucose transport in 3T3-L1 adipocytes. It was reported that PDGF has no significant effect or only a minimal effect on glucose transport in 3T3-L1 adipocytes (14, 15), and other workers (16-18) suggested that PDGF can stimulate glucose uptake by the translocation of GLUT1 instead of GLUT4, as based on findings that PDGF had no detectable effect on GLUT4 translocation in 3T3-L1 adipocytes, determined using fractionation procedures or photoaffinity labeling. They found that PDGF did not stimulate GLUT4 translocation even though PDGF did stimulate PI 3-kinase to an extent similar to that seen with insulin in 3T3-L1 adipocytes; they proposed that activation of PI 3-kinase alone is not sufficient to stimulate GLUT4 translocation to the plasma membrane (14, 18).

To understand better the pathophysiology of insulin-resistant states, it is crucial to identify key molecular components necessary to mediate insulin signaling. Since insulin and PDGF are growth factors, there are overlaps between signal transduction pathways. Understanding the effect of PDGF on glucose transport may aid in identifying insulin signaling pathway mediating the glucose transport. Murine 3T3-L1 cells express both insulin and PDGF receptors and represent an in vitro model for insulin-induced glucose transport (19). To extend our previous study on the effect of PDGF on glucose transport and the role of PI 3-kinase in the PDGF-induced glucose transport, we compared the effects of insulin and PDGF on the stimulation of glucose transport, translocations of glucose transporters and PI 3-kinase activity in 3T3-L1, 3T3-L1GLUT4MYC, and 3T3-L1GLUT1MYC adipocytes. We found that 1) PDGF stimulated glucose uptake in both 3T3-L1 fibroblasts and adipocytes, 2) PDGF-induced glucose uptake was shared by GLUT4 and GLUT1, and 3) PDGF stimulated the translocation of GLUT4 to the plasma membrane in 3T3-L1 adipocytes transiently by a PI 3-kinase-dependent pathway.

    EXPERIMENTAL PROCEDURES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Materials-- Insulin was purchased from Sigma and PDGF-BB was purchased from PeproTech Ltd. (London, UK). Dulbecco's modified Eagle's medium was obtained from Nissui Pharmaceutical Co., Ltd. (Tokyo, Japan). Calf bovine serum was obtained from ICN Biomedicals, Inc. (Costa Mesa, CA), and fetal bovine serum was obtained from Life Technologies, Inc. A hybridoma of monoclonal antibody (9E10) against human c-MYC was obtained from the American Type Culture Collection. Antibodies to C terminus of GLUT4 were prepared by immunizing rabbit with C-terminal 30 amino acids of rat GLUT4. Antibodies to C terminus of GLUT1 were purchased from Chemicon International Inc. (Temecula, CA). Secondary horseradish peroxidase-conjugated goat anti-mouse IgG and anti-rabbit IgG were obtained from BioSource (Camarillo, CA). An anti-phosphotyrosine antibody (PY20) was purchased from Transduction Laboratories (Lexington, KY). Tyramide signal amplification kit (green) for immunohistochemistry was purchased from NEN Life Science Products. 2-Deoxy-[1,2-3H]D-glucose, was obtained from Moravek Biochemicals. Inc. (Brea, CA). All other biochemicals were obtained from Sigma.

Cell Culture-- 3T3-L1 murine fibroblasts were obtained from the American Type Culture Collection (Rockville, MD). The cell lines used in this study were 3T3-L1 fibroblasts, 3T3-L1GLUT4MYC, a 3T3-L1 fibroblast line stably overexpressing GLUT4MYC, constructed by inserting a human c-MYC epitope (14 amino acids) into the first ectodomain of GLUT4, and 3T3-L1-GLUT1MYC, a 3T3-L1 fibroblast line stably overexpressing GLUT1MYC (9, 20). The 3T3-L1, 3T3-L1-GLUT4MYC, and 3T3-L1-GLUT1MYC fibroblasts were cultured in Dulbecco's modified Eagle's medium, 10% calf bovine serum and induced to differentiate into adipocytes, as described (21). The adipocytes were used between 7 and 9 days after the initiation of differentiation, a time when more than 90% of the cells had an adipocyte phenotype.

Western Blot Analyses of GLUTMYC Protein-- Subcellular fractions from 3T3-L1, 3T3-L1GLUT4MYC, and 3T3-L1GLUT1MYC adipocytes were prepared as described (20). Equal amounts of protein from the same fraction number of different cells were resolved by 10% SDS-polyacrylamide gel electrophoresis and transferred by electroblotting onto nitrocellulose membrane (Schleicher & Schuell). Blots were probed with mouse monoclonal antibodies to the human c-MYC epitope of 14 amino acids (9E10), rabbit polyclonal antibodies to C terminus of GLUT4, or rabbit polyclonal antibodies to C terminus of GLUT1. Proteins were visualized using enhanced chemiluminescence (ECL, Amersham Pharmacia Biotech).

Cell Surface Anti-c-MYC Antibody Binding Assay (GLUTMYC Translocation Assay)-- The 3T3-L1GLUT4MYC or 3T3-L1GLUT1MYC cells in 24-well plates were incubated in 500 µl of Krebs-Ringer/Hepes (KRH) buffer (136 mM NaCl, 4.7 mM KCl, 1.25 mM CaCl2, 1.25 mM MgSO4, 20 mM Hepes, pH 7.4, and 2 mg/ml bovine serum albumin) for 30 min at 37 °C and then with indicated concentrations of ligands for indicated periods at 37 °C. GLUTMYC translocation was measured, as described (9).

2-Deoxyglucose Uptake Measurement-- Cells cultured in Dulbecco's modified Eagle's medium, 10% fetal calf serum medium with 24-well plates were washed once with KRH buffer and incubated for 30 min in KRH buffer and then treated with indicated concentrations of ligands for 10 min at 37 °C. 2-Deoxy[3H]glucose uptake was measured by incubating cells with the deoxy[3H]glucose for 10 min, as described (22, 23).

Sudan III Stain-- Cells in a 6-well plate were incubated with 5 ml of 50% ethanol for 2 min at room temperature and then stained with 1 ml of 2% Sudan III in 70% ethanol for 30 min at 37 °C. After washing the cells once with 5 ml of 50% ethanol and twice with 5 ml of H2O at room temperature, photographs were taken.

Immunostaining and Fluorescence Microscopy-- GLUTMYC protein staining was done using the tyramide signal amplification-direct procedure following the manufacturer's instructions (NEN Life Science Products). Briefly, 3T3-L1, 3T3-L1GLUT4MYC, and 3T3-L1GLUT1MYC cells were grown in a 6-well plate and converted to adipocytes. The adipocytes were incubated in 1 ml of KRH buffer for 30 min at 37 °C, and then indicated concentrations of ligands were added for 10 min at 37 °C. After fixation with 2% paraformaldehyde/phosphate-buffered saline (137 mM NaCl, 8.1 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) at room temperature for 20 min, cells were washed three times with phosphate-buffered saline, treated with 100 mM glycine/phosphate-buffered saline at room temperature for 15 min, and incubated with TNB blocking buffer (0.1 M Tris-HCl, pH 7.5, 0.15 M NaCl, 0.5% Blocking Reagent supplied in a kit) at room temperature for 30 min. They were then incubated with the anti-c-MYC antibodies (1:20 dilution with TNB blocking buffer) at 37 °C for 30 min and then further incubated with horseradish peroxidase-conjugated goat anti-mouse IgG (1:20 dilution with TNB blocking buffer) at 37 °C for 30 min. Each antibody incubation was followed by three washes for 5 min each with TNT buffer (0.1 M Tris-HCl, pH 7.5, 0.15 M NaCl, 0.05% Tween 20), at room temperature. Cells then were incubated with fluorophore tyramide (1:50 dilution with amplification buffer) for 7 min at room temperature in a dark, moist chamber, washed three times for 10 min each in TNT buffer at room temperature and visualized under a fluorescence microscope (Olympus BX50).

PI 3-Kinase Assay-- Preparation of cell lysates and PI 3-kinase assay were done as described (24). Briefly, total cell lysates were prepared with buffer containing 1% Nonidet P-40 after treatment with the indicated concentrations of insulin or PDGF and precipitated with the PY20 antibodies and protein A-Sepharose CL-4B (Amersham Pharmacia Biotech). The immunoprecipitates were subjected to PI 3-kinase assay. The radioactive spots on TLC plates were quantified using a Bio-image analyzer BAS1500 (Fuji Film Institution).

    RESULTS
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Effects of PDGF and Insulin on Glucose Uptake in 3T3-L1 Fibroblasts and Adipocytes-- To determine whether PDGF has any effect on glucose uptake, PDGF-induced glucose uptake was investigated using both 3T3-L1 fibroblasts and 3T3-L1 adipocytes. The upper panel of Fig. 1A shows the result of Sudan III staining. Few lipid droplets was seen in fibroblasts, and many lipid droplets appeared after induction, suggesting the cells were well differentiated into adipocytes. The lower panel of Fig. 1A shows the effects of PDGF and insulin on glucose uptake. In 3T3-L1 fibroblasts, a similar stimulation of glucose uptake was induced by PDGF (about 2-fold) and insulin (about 2-fold), an effect due to the translocation of GLUT1 from an intracellular pool to the plasma membrane (25, 26). In 3T3-L1 adipocytes, however, PDGF exerted a 6-fold stimulation of glucose uptake, even though this stimulation reached only 60% of the maximal effect of insulin (10-fold). This suggested that another glucose transporter, probably GLUT4, may be involved in the PDGF-induced glucose uptake, in addition to GLUT1, since the translocation of GLUT1 only caused about 2-fold stimulation of glucose uptake (27). To eliminate the possibility that the 3T3-L1 cells from different origins give different responses to PDGF, we also determined the PDGF-induced glucose uptake in another 3T3-L1 cell line (kindly provided by Dr. Morris Birnbaum, University of Pennsylvania). This 3T3-L1 cell line (named 3T3-L1-B) responded similarly to our 3T3-L1 cells (Fig. 1, A and B).


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  		Fig. 1.   Effect of PDGF and insulin on glucose uptake in 3T3-L1 fibroblasts and adipocytes. 3T3-L1 cells (used in our laboratory, A) and 3T3-L1-B cells (obtained from Professor Birnbaum of the University of Pennsylvania, B) were used in glucose uptake. Upper panels of A and B show the result of Sudan III staining performed as described under "Experimental Procedures." The lower panels shows the effects of PDGF and insulin on glucose uptake. Cells were stimulated with 100 nM insulin or 50 ng/ml PDGF-BB for 10 min at 37 °C. The 2-deoxyglucose uptake was measured as described under "Experimental Procedures." Values represent means ± S.E. of three separate experiments done in triplicate.

To confirm whether GLUT4 was responsible for the PDGF-induced glucose uptake, 3T3-L1 adipocytes stably overexpressing either GLUT4MYC or GLUT1MYC were evaluated.

GLUTMYC Translocation and Glucose Uptake by PDGF and Insulin-- To examine which glucose transporter is responsible for the PDGF-induced glucose uptake, 3T3-L1 cell lines stably overexpressing either GLUT4MYC or GLUT1MYC were established and cell-surface anti-c-MYC antibody binding assay was done. As shown in Fig. 2A, the overexpression of GLUT4MYC or GLUT1MYC in 3T3-L1 cells was confirmed by immunoblotting with anti-c-MYC antibodies. In 3T3-L1GLUT4MYC adipocytes, the total expression of GLUT4 (endogenous GLUT4 plus GLUT4MYC) was approximately 2.5-fold over control (3T3-L1 adipocytes) as detected with antibodies C-terminal to GLUT4. In 3T3-L1GLUT1MYC adipocytes, the total GLUT1 (endogenous GLUT1 plus GLUT1MYC) was increased by 7-10-fold compared with endogenous GLUT1 as determined with antibodies C-terminal to GLUT1. After transfection with GLUT4MYC or GLUT1MYC, the adipocytes showed an enhanced increase in insulin- or PDGF-induced glucose transport compared with parent 3T3-L1 cells (Fig. 2B). As shown in Fig. 2C, PDGF led to about a 5-fold increase of GLUT4MYC translocation in 3T3-L1 GLUT4MYC adipocytes and a 2-fold increase of GLUT1MYC translocation in 3T3-L1 GLUT1MYC adipocytes, whereas the parent 3T3-L1 adipocytes showed no response to either PDGF or insulin in the anti-c-MYC binding assay. These results suggest that PDGF can trigger the translocation of both GLUT4 and GLUT1 to the plasma membrane and exert the stimulation of glucose uptake in 3T3-L1 adipocytes. Also, the PDGF-stimulated GLUT4MYC translocation and glucose uptake were in a dose-dependent manner in 3T3-L1 GLUT4MYC adipocytes. However, PDGF only induced about 60% maximal effect of insulin on glucose transport with 10 min stimulation (Fig. 3).


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  		Fig. 2.   Expression of GLUT4MYC and GLUT1MYC and effects of PDGF and insulin on both transporter translocation and glucose uptake in 3T3-L1GLUT1MYC and 3T3-L1GLUT4 MYC adipocytes. A, immunoblot of microsomal fractions from 3T3-L1 adipocytes and 3T3-L1-adipocytes stably expressing GLUT4MYC or GLUT1MYC were performed with the anti-c-MYC monoclonal antibodies (Anti-c-MYC) and polyclonal antibodies C-terminal to GLUT4 or GLUT1 as described under "Experimental Procedures." The locations of glucose transporter (GLUT, around Mr 55,000) are indicated. 3T3-L1 adipocytes, 3T3-L1GLUT4MYC, or 3T3-L1GLUT1MYC adipocytes were stimulated with 100 nM insulin or 50 ng/ml PDGF-BB for 10 min at 37 °C. The 2-deoxyglucose uptake (B) and GLUTMYC translocation (C) were measured as described under "Experimental Procedures." Values represent means ± S.E. of three separate experiments done in triplicate.


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  		Fig. 3.   Dose-dependent GLUTMYC translocation and glucose uptake in response to insulin or PDGF in 3T3-L1GLUT4MYC and 3T3-L1GLUT1MYC adipocytes. Adipocytes were incubated with various concentrations of insulin or PDGF-BB for 10 min at 37 °C. The 2-deoxyglucose uptake (A and C) and GLUTMYC translocation (B and D) were measured as described under "Experimental Procedures." Values represent means ± S.E. of three separate experiments done in triplicate.

Immunostaining and fluorescence microscopy were also used to determine further the effect of PDGF on translocation of glucose transporters in 3T3-L1GLUT4MYC and 3T3-L1GLUT1MYC adipocytes. As shown in Fig. 4, an increase in plasma membrane-associated fluorescence staining, as visualized as a ring around the cell, was induced by PDGF and insulin in both 3T3-L1GLUT4MYC and 3T3-L1GLUT1MYC adipocytes, whereas the controls showed only nonspecific staining without a enhanced staining in plasma membrane, indicating that PDGF stimulated translocations of GLUT4MYC and GLUT1MYC to the plasma membrane in 3T3-L1 adipocytes. As shown in Fig. 4, F and I, insulin triggered GLUT4MYC translocation in almost all the adipocytes. However, approximately 60% of cells showed positive staining with PDGF treatment, compared with findings in the case of insulin stimulation (Fig. 4, D-I) (see "Discussion"). These results were consistent with findings that PDGF induced approximately 60% of maximal effects of insulin on glucose uptake and translocation (Figs. 2 and 3).


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  		Fig. 4.   Immunofluorescence microscopy of GLUTMYC translocation in 3T3-L1-GLUT1MYC and 3T3-L1-GLUT4MYC adipocytes. The cells were grown in a 6-well plate and processed for immunofluorescence microscopy as described under "Experimental Procedures." 3T3-L1 adipocytes (A, B, and C, × 40 lens, 40 s), 3T3-L1-GLUT4MYC adipocytes (D, E, and F, × 10 lens, 2 min; G, H, and I, × 40 lens, 1 min), and 3T3-L1-GLUT1MYC adipocytes (J, K, and L, × 40 lens, 2 s) were stimulated with 50 ng/ml PDGF-BB (B, E, H, and K) or 100 nM insulin (C, F, I, and L) or buffer alone (A, D, G, and J) for 10 min at 37 °C and stained with anti-c-MYC antibody followed by incubation with horseradish peroxidase-conjugated goat anti-mouse IgG antibody. Cells positive for GLUT4MYC or GLUT1MYC translocation show an increase in plasma membrane-associated fluorescein staining that is visualized as a ring around the cell. Control cells (3T3-L1 adipocytes) showed only a nonspecific staining without a enhanced staining in plasma membrane.

Taken together, these results suggested that PDGF does exert stimulation of the 2-deoxyglucose uptake, and the PDGF-induced glucose uptake was shared by GLUT4 and GLUT1 transporters in 3T3-L1 adipocytes.

Transient Effect of PDGF on GLUT4MYC Translocation and PI 3-Kinase Activation in 3T3-L1 Adipocytes-- It was reported that PDGF transiently activated PI 3-kinase in 3T3-L1 adipocytes (28). We reported that PDGF triggered GLUT4 translocation by a PI 3-kinase-dependent signaling pathway in CHO cells (11). To investigate the relationship between glucose transport and PI 3-kinase activity stimulated by insulin and PDGF in 3T3-L1 adipocytes, the time courses for insulin- and PDGF-induced glucose uptake, translocations of transporters, and PI 3-kinase activity were studied using 3T3-L1GLUT4MYC and 3T3-L1GLUT1MYC adipocytes. In 3T3-L1GLUT4MYC adipocytes, as shown in Fig. 5, A, B and E, PDGF-induced glucose uptake, GLUT4MYC translocation, and PI 3-kinase activity were maximal (100% of maximal effects of PDGF) at 5-10 min and thereafter rapidly declined to 30, 40, and 12%, respectively, within 60 min, a time when maximal effects of insulin on glucose uptake, GLUT4MYC translocation, and PI 3-kinase activity were observed. On the other hand, even though the maximal activity of PI 3-kinase induced by PDGF was about 2.1-fold higher than that stimulated by insulin (Fig. 5E), the PDGF stimulated less glucose uptake and GLUT4MYC translocation compared with that stimulated by insulin in 3T3-L1GLUT4MYC adipocytes (Figs. 2 and 3). This result suggested that the different ability of insulin and PDGF to activate PI 3-kinase and PI 3-kinase activation only is not sufficient to mediate the full effect of insulin on glucose transport (see "Discussion").


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  		Fig. 5.   Time-dependent GLUTMYC translocation, glucose uptake, and PI 3-kinase activation in response to insulin or PDGF in 3T3-L1-GLUT1MYC and 3T3-L1-GLUT4MYC adipocytes. Adipocytes were incubated with 100 nM insulin or 50 ng/ml PDGF-BB for the indicated periods at 37 °C. The 2-deoxyglucose uptake (A and C) and GLUTMYC translocation (B and D) were measured as described under "Experimental Procedures." Values represent means ± S.E. of three separate experiments done in triplicate. E, the cell lysates were immunoprecipitated with an anti-Tyr(P) antibody (PY20) and subjected to PI 3-kinase assay as described under "Experimental Procedures." Values represent means ± S.E. of three separate experiments. The percent of maximal effects refers to percent of maximal effects of PDGF or insulin, respectively.

Some differences in GLUT1MYC translocation and glucose uptake were observed in case of 3T3-L1GLUT1MYC adipocytes. As shown in Fig. 5, C and D, PDGF-induced GLUT1MYC translocation and glucose uptake reached maximal levels (100%) at 5-10 min and then declined slowly to 85 and 65%, respectively, within 60 min. In the insulin-stimulated state, the maximal levels of GLUT1MYC translocation and glucose uptake were observed to occur within 60 min, similar to findings in case of 3T3-L1GLUT4MYC adipocytes. Therefore, there are some differences between insulin-induced and PDGF-induced translocations of glucose transporters and different regulations in recycling of GLUT4 and GLUT1 glucose transporters.

PDGF stimulated the parallel translocations of glucose transporters and activation of PI 3-kinase in time courses, thereby suggesting the involvement of PI 3-kinase in PDGF-induced glucose transport. To confirm this, wortmannin was used in the experiments.

Effects of Wortmannin on PDGF-induced GLUT4MYC Translocation and Glucose Uptake-- Wortmannin is a specific and cell-permeable inhibitor of PI 3-kinase activity and is used widely in PI 3-kinase-related research (29). To determine if PI 3-kinase is involved in PDGF-induced GLUT4 translocation, wortmannin treatment was given prior to PDGF stimulation. As shown in Fig. 6, pretreatment with 0.1 µM wortmannin abolished PDGF-induced GLUT4MYC translocation and glucose uptake in 3T3-L1GLUT4MYC adipocytes. Therefore, effects of PDGF on GLUT4 translocation and glucose uptake depend on PI 3-kinase activity in 3T3-L1 adipocytes.


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  		Fig. 6.   Effects of wortmannin on insulin- or PDGF-stimulated GLUT4MYC translocation and glucose uptake in 3T3-L1-GLUT4MYC adipocytes. 3T3-L1GLUT4MYC adipocytes were preincubated with buffer or 0.1 µM wortmannin for 10 min at 37 °C and then were stimulated with 100 nM insulin, 50 ng/ml PDGF-BB, or buffer alone for 10 min at 37 °C. The 2-deoxyglucose uptake (A) and GLUT4MYC translocation (B) were measured as described under "Experimental Procedures." Values represent means ± S.E. of three separate experiments done in triplicate.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
REFERENCES

Insulin stimulates GLUT4 translocation and glucose uptake by a PI 3-kinase-dependent signaling pathway. We and others (10-13) have demonstrated that PDGF and EGF can also stimulate GLUT4 translocation and glucose uptake by a wortmannin-sensitive pathway in CHO cells and adipocytes. To investigate further the effect of PDGF on glucose transport, we examined glucose uptake and translocation of glucose transporters stimulated by PDGF in 3T3-L1 adipocytes that stably overexpressed either GLUT4MYC or GLUT1MYC.

Our observations strongly support the conclusion that PDGF can stimulate the translocation of GLUT4 and glucose uptake in 3T3-L1 adipocytes. First, when 3T3-L1 adipocytes were stimulated with PDGF, a 6.5-fold stimulation of the 2-deoxyglucose uptake was observed, whereas the translocation of GLUT1 caused only a 2-3-fold increase of glucose uptake (27). In addition, we also found that only a 2.5-fold increase of the 2-deoxyglucose uptake was obtained by PDGF treatment in 3T3-L1GLUT1MYC adipocytes (Fig. 2B). To eliminate the possibility that 3T3-L1 cells from different origins give different responses to PDGF and PDGF obtained from different origins causes different reactions, the 3T3-L1 cells and PDGF-BB from other origins also were used in experiments. Similar results of glucose uptake induced by PDGF were obtained using our cells (3T3-L1) and those of other investigators (3T3-L1-B) (Fig. 1, A and B). A 5-fold stimulation of the glucose uptake was also induced by PDGF-BB obtained from another provider (Austral Biological) in our 3T3-L1 adipocytes (data not shown). These results confirmed that there was no problem in the 3T3-L1 cell line and the PDGF-BB product used in present study. Thus, it is reasonable to assume that GLUT4 is involved in PDGF-induced glucose uptake. Second, exogenous GLUT4MYC expression led to enhanced PDGF-induced glucose transport. About double the amount of glucose uptake was stimulated by PDGF in 3T3-L1GLUT4MYC adipocytes, compared with that observed in parent 3T3-L1 adipocytes(Fig. 2B). However, overexpressing GLUT4MYC did not increase the fold stimulation induced by PDGF or insulin. In 3T3-L1GLUT4MYC adipocytes, PDGF stimulated a 5.7-fold increase in glucose uptake similar to that observed in parent 3T3-L1 adipocytes, which meant that the exogenous GLUT4MYC was translocated through the same mechanism by which the endogenous GLUT4 transporters were translocated. Furthermore, cell-surface anti-c-MYC antibody binding assay showed that PDGF induced a 5-fold increase of GLUT4MYC translocation in 3T3-L1GLUT4MYC adipocytes and a 2-fold increase of GLUT1MYC translocation in 3T3-L1GLUT1MYC adipocytes (Fig. 2C). Finally, immunostaining and fluorescence microscopy also showed an increase in plasma membrane-associated fluorescence staining after treatment with PDGF in 3T3-L1GLUT4MYC adipocytes (Fig. 4). These results strongly indicate that PDGF can stimulate the translocation of GLUT4 transporter and glucose uptake in 3T3-L1 adipocytes.

The present study suggested that the effect of PDGF on glucose uptake is transient and reaches the peak between 5 and 10 min after exposure to PDGF in 3T3-L1 adipocytes. It was reported that PDGF had only a limited effect on glucose uptake based on the experiments done with 15 min exposure to PDGF after 3 h incubation of KRBH/BSA buffer (14) or 20 min exposure to PDGF after overnight starvation of serum-free medium (18). It was possible to obtain more glucose uptake induced by PDGF in their cells if a shorter exposure to PDGF was performed. Furthermore, we only incubated the adipocytes with KRH buffer for 30 min before PDGF stimulation. The differences in the condition of the cell and the experimental procedure may contribute to the discrepancy in addition to the length of exposure to PDGF.

Our present results are inconsistent with data that showed that PDGF had no detectable effect on GLUT4 translocation and stimulated glucose uptake only by activation of GLUT1 transporter in 3T3-L1 adipocytes (16-18), determined using the sucrose density gradient method. With this method, we also detected no significant increase of GLUT4MYC translocation in 3T3-L1GLUT4MYC adipocytes; there was only a significant increase in GLUT1MYC translocation after treatment with PDGF in 3T3-L1GLUT1MYC adipocytes (data not shown). By using MYC-binding assay, however, we found that PDGF can transiently trigger the translocation of GLUT4MYC transporter and glucose uptake in 3T3-L1GLUT4MYC adipocytes. This transient effect of PDGF on glucose transport may be due to transient autophosphorylation of PDGFR and transient activation of PI 3-kinase induced by PDGF (28). The time courses for PDGF-induced translocations of GLUT4MYC and GLUT1MYC showed that PDGF had a longer effect on the translocation of GLUT1 transporter (Fig. 5), which meant that there were some differences between the mechanisms by which PDGF stimulated the GLUT4 and GLUT1 redistribution. The GLUT1 transporters may have a slower endocytosis rate. Because fractionation procedures are time-consuming and relatively insensitive, it seems difficult to detect the transient GLUT4 translocation induced by PDGF by using this method. But PDGF-induced GLUT1 translocation can be detected, even using this method, for the reason mentioned above. Differences in time after PDGF treatment, detection methods of GLUT4 translocation, and the days after differentiation of adipocytes may account for the discrepancy between the results.

Evidence suggests that PI 3-kinase is both necessary and sufficient for the metabolic effects of insulin (30, 31). PI 3-kinase also is strongly activated by PDGF. In the present study, PDGF led to similar time courses of glucose uptake, GLUT4 translocation, and the activation of PI 3-kinase in 3T3-L1 adipocytes (Fig. 5). Wortmannin, a specific inhibitor of PI 3-kinase, abolished the PDGF-induced GLUT4 translocation and glucose uptake in 3T3-L1 adipocytes (Fig. 6). These results confirmed that PDGF-induced GLUT4 translocation and glucose uptake are mediated by a PI 3-kinase-dependent signaling pathway in 3T3-L1 adipocytes. It was suggested that PDGF-induced translocation of GLUT1 is also PI 3-kinase-dependent, based on the result that PDGF-induced glucose uptake was completely inhibited by wortmannin in 3T3-L1 adipocytes. Our previous study indicated that PDGF-stimulated GLUT4MYC translocation was mediated by PI 3-kinase and phospholipase Cgamma pathways and that the PDGF-stimulated GLUT4MYC translocation remained a little after treatment with wortmannin in CHO-GLUT4MYC-PDGFR cells. The expression level of PDGFR is very high in the CHO-GLUT4MYC-PDGFR cells and markedly decreased in the 3T3-L1 adipocytes (32, 33). So the discrepancy between present and previous results may be accounted for by different expression levels of PDGFR in CHO-PDGFR cells and 3T3-L1 adipocytes.

As to why PDGF only induced about 60% of the maximal effect of insulin on glucose transport in 3T3-L1 adipocytes, we think there are at least two possible explanations for this discrepancy. One possibility is that PI 3-kinase activation only is not sufficient to mediate the full effect of insulin on glucose transport, a notion supported by findings that a thiophosphotyrosine peptide treatment (34) or adenovirus-mediated expression of constitutively active PI 3-kinase in 3T3-L1 adipocytes (35) stimulated glucose uptake less than the treatment with insulin, even though the same levels of PI 3-kinase activation were induced. The other possibility is that the decreased expression of PDGF receptors is responsible for the inability of PDGF to exert a full insulin effect on glucose uptake in 3T3-L1 adipocytes. Evidence showed that levels of mRNA and protein of PDGF receptors are markedly decreased after differentiation of 3T3-L1 fibroblasts into adipocytes (32, 33). On the other hand, insulin receptor number was increased after differentiation (36). We considered that 3T3-L1 adipocytes are heterogeneous concerning the number of PDGF receptors on the cell surface; approximately 40% of the adipocytes have few PDGF receptors and do not visibly respond to PDGF. This may explain why PDGF triggered GLUT4MYC translocation only in 60% of adipocytes (Fig. 4, E and H). To confirm this further, we wanted to detect the expression levels of PDGF receptors on the surface of single 3T3-L1 adipocyte with immunostaining and fluorescence microscopy. However, this approach was not feasible as antibody to the extracellular domain of mouse PDGFR was not available. One or both of the above possibilities may account for the different stimulations of glucose transport induced by PDGF and insulin in 3T3-L1 adipocytes.

It was demonstrated that PDGF or EGF can activate Akt and PKClambda through phosphatidylinositol 3-kinase (37-39). Subsequently, studies indicated that activation of Akt and PKClambda was involved in insulin-stimulated glucose uptake and GLUT4 translocation in 3T3-L1 adipocytes (40, 41). These results also support the possibility that PDGF can stimulate glucose uptake and GLUT4 translocation through activation of Akt or PKClambda in 3T3-L1 adipocytes. Growth factors, such as insulin, PDGF, and EGF, may exert effects on glucose transport in cells through a common signaling pathway.

Both insulin and PDGF can stimulate GLUT4 translocation through a wortmannin-sensitive pathway, without the involvement of insulin receptor substrate-1 (42, 43), and activate many of the same signaling cascades. Cross-talk between the signaling pathways stimulated by PDGF and insulin have been reported (44, 45). Clarifying effects of PDGF on glucose transport should lead to a better understanding of effects of insulin on glucose transport in physiological situations and in the presence of diabetes.

    ACKNOWLEDGEMENTS

We thank Drs. T. Ohnishi and T. Imanaka for technical assistance and M. Ohara for reading the manuscript.

    FOOTNOTES

* This work was supported by research grants from the Ministry of Education, Science, Sports and Culture of Japan, for diabetes research from the Ministry of Health and Welfare and from Otsuka Pharmaceutical, Tokushima, "The Mitsubishi Foundation" (to Y. E.), the Japan Diabetes Foundation (to H. H.), "The Kato Memorial Bioscience Foundation" (to H. H.), and "The Meiji Life Foundation of Health and Welfare" (to H. H.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Division of Molecular Genetics, Institute for Enzyme Research, The University of Tokushima, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan. Tel.: 81-88-633-7435; Fax: 81-88-633-7437; E-mail: ebina{at}ier.tokushimau.ac.jp.

    ABBREVIATIONS

The abbreviations used are: GLUT4, glucose transporter type 4; GLUT1, glucose transporter type 1; PI, phosphatidylinositol; CHO, Chinese hamster ovary; PDGF, platelet-derived growth factor; PDGFR, PDGF receptor; EGF, epidermal growth factor.

    REFERENCES
TOP
ABSTRACT
INTRODUCTION
EXPERIMENTAL PROCEDURES
RESULTS
DISCUSSION
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