J Biol Chem, Vol. 274, Issue 27, 19309-19315, July 2, 1999

Identification of Amino Acids That Modulate Mannose Phosphorylation of Mouse DNase I, a Secretory Glycoprotein^*

Atsushi Nishikawa, Akash Nanda, Walter Gregory, John Frenz^§, and Stuart Kornfeld^¶

From the Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, the  Department of Biochemistry, Okayama University of Science, Okayama 700, Japan, and ^§ Genentech, Inc., South San Francisco, California 94080

	ABSTRACT

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

We have reported that bovine DNase I, a secretory glycoprotein, acquires mannose 6-phosphate residues on 12.6% of its Asn-linked oligosaccharides when expressed in COS-1 cells and that the extent of phosphorylation increases to 79.2% when lysines are placed at positions 27 and 74 of the mature protein (Nishikawa, A., Gregory, W., Frenz, J., Cacia, J., and Kornfeld, S. (1997) J. Biol. Chem. 272, 19408-19412). We now demonstrate that murine DNase I, which contains Lys²⁷ and Lys⁷⁴, is phosphorylated only 20.9% when expressed in the same COS-1 cell system. This difference is mostly due to the absence of three residues present in bovine DNase I (Tyr⁵⁴, Lys¹²⁴, and Ser¹⁹⁰) along with the presence of a valine at position 23 that is absent in the bovine species. We show that Val²³ inhibits phosphorylation at the Asn¹⁸ glycosylation site, whereas Tyr⁵⁴, Lys¹²⁴, and Ser¹⁹⁰ enhance phosphorylation at the Asn¹⁰⁶ glycosylation site. Tyr⁵⁴ and Ser¹⁹⁰ are widely separated from each other and from Asn¹⁰⁶ on the surface of DNase I, indicating that residues present over a broad area influence the interaction with UDP-GlcNAc:lysosomal enzyme N-acetylglucosamine-1-phosphotransferase, which is responsible for the formation of mannose 6-phosphate residues on lysosomal enzymes.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

In a previous report, we demonstrated that bovine DNase I, a secretory glycoprotein of the pancreas and the salivary gland, acquires mannose 6-phosphate moieties on its Asn-linked oligosaccharides (1), confirming the findings of Cacia et al. (2) with human DNase I. However, the level of oligosaccharide phosphorylation (12.6%) was considerably less than that observed with lysosomal hydrolases (usually 50% or greater). This indicated that DNase I is a weak substrate for UDP-GlcNAc:lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (phosphotransferase),¹ the enzyme that synthesizes the Man-6-P recognition marker for targeting to lysosomes. Phosphotransferase is known to recognize a conformation-dependent protein determinant that is present in lysosomal hydrolases, but absent in most secretory glycoproteins (3). This determinant has been shown to involve a broad surface patch that includes critical lysine residues (4-11). The binding of lysosomal hydrolases to phosphotransferase is followed by the transfer of GlcNAc-P from UDP-GlcNAc to selected mannose residues on the Asn-linked high mannose oligosaccharides of the hydrolases. The N-acetylglucosamine is then removed by N-acetylglucosamine-1-phosphodiester--N-acetylglucosaminidase to generate the phosphomannosyl recognition determinant that allows binding to the mannose 6-phosphate receptors in the Golgi and subsequent targeting to lysosomes (12). The basal level of phosphorylation of bovine DNase I was shown to be dependent on three residues (Lys⁵⁰, Lys¹²⁴, and Arg²⁷), consistent with the finding that lysine residues are important components of the phosphotransferase recognition determinant on lysosomal hydrolases (1). When Arg²⁷ was replaced with a lysine, oligosaccharide phosphorylation increased to 54%, demonstrating that phosphotransferase prefers lysine residues over arginines. Furthermore, mutation of Asn⁷⁴ to a lysine also increased phosphorylation to 50.3%, and the double mutant (R27K/N74K) was phosphorylated 79.2%, equivalent to the values observed with authentic lysosomal hydrolases. Interestingly, the lysine at position 27 specifically stimulated phosphorylation of the oligosaccharide at Asn¹⁸, whereas the lysine at position 74 selectively stimulated phosphorylation of the oligosaccharide at Asn¹⁰⁶.

The R27K and N74K bovine constructs were initially prepared because the murine DNase I sequence has lysine residues at these positions, along with a lysine at position 50 (a glutamate is present at position 124). Yet in preliminary experiments we found that the murine DNase I was phosphorylated only 21% when expressed in the standard COS-1 cell system. We reasoned that this relatively poor phosphorylation might indicate that murine DNase I either lacks amino acids present in bovine DNase I that are necessary for efficient phosphorylation or contains amino acids that impair mannose phosphorylation. We also considered the possibility that both situations might hold. Our first clue that particular amino acid residues may impair phosphorylation came from the finding that substitution of Lys¹¹⁷ with an alanine in the bovine DNase I molecule enhanced phosphorylation by greater than 50% (1). In this paper, we identify five amino acids that modulate the phosphorylation of murine DNase I to a large extent. Two residues (Val²³ and Lys¹¹⁷) are present in the murine sequence and impair phosphorylation. The other three residues (Tyr⁵⁴, Lys¹²⁴, and Ser¹⁹⁰) are found in bovine DNase I but not murine DNase I and stimulate phosphorylation. Together these findings provide new insight into the nature of the phosphotransferase recognition domain.

	EXPERIMENTAL PROCEDURES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Materials-- COS-1 cells were obtained from ATCC. [2-³H]Mannose was purchased from NEN Life Science Products. QAE-Sephadex, Concanavalin A-Sepharose, and the pSVK3 expression vector were from Amersham Pharmacia Biotech. Recombinant endoglycosidase H fused to maltose-binding protein and protein A-agarose beads were from New England Biolabs and Repligen, respectively. Bovine calf serum, Lipofectin, and Opti-MEM were from Life Technologies, Inc. Other reagents were purchased from Sigma.

Plasmids-- Bovine DNase I cDNA lacking a signal sequence (13) was kindly provided by Dr. D. Suck (EMBL, Heidelberg, Germany). This cDNA was inserted into the pSVK3 expression vector behind the signal sequence of human DNase I as described (1). The cDNA encoding murine DNase I was obtained from Dr. Susan Spencer (Genentech). The constructs encoding chimeric proteins between bovine and murine DNase I were prepared using the sequential polymerase chain reaction procedure (14). The expression vectors containing the various mutations in the murine DNase I were also constructed by this procedure. All plasmids used for transfections were purified with the Qiagen plasmid kit.

COS-1 Cell Transfections-- COS-1 cells were cultured in Dulbecco's modified Eagle's medium containing 10% calf serum. The cells were transfected with purified plasmid DNA using the Lipofectin reagent and then labeled with [2-³H]mannose in the presence of 10 mM NH₄Cl as described (1).

Immunoprecipitation and Oligosaccharide Analysis-- Rabbit anti-bovine DNase I serum was prepared by immunizing a rabbit with bovine DNase I purchased from Sigma and purified one additional step on a concanavalin A-Sepharose column. Rabbit anti-murine DNase I was provided by Dr. Jerry Cacia (Genentech). The harvested COS-1 culture medium containing the [2-³H]mannose-labeled secreted proteins was incubated with 2 µl of antisera overnight with rotation at 4 °C. Then, 100 µl of a 50% protein A-agarose bead suspension was added. After 1 h of additional rotation, the bead suspension was transferred to a fresh tube. The beads were washed three times with 100 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% Triton X-100, 5 mM EDTA, and 10 trypsin inhibitory units/ml aprotinin. The immunoprecipitated protein was eluted from the beads in 50 µl of 0.5% SDS-10 mM -mercaptoethanol by heating at 78 °C for 10 min and rinsing the beads with 50 µl of water.

Analysis of the oligosaccharides of the immunoprecipitated DNase I and the calculation of the percent of the oligosaccharides phosphorylated with one or two phosphate groups was carried out according to the previously described methods (8).

Determination of Specific Activity-- COS cells were transfected with the various plasmid DNAs as described (1). Forty-eight hours posttransfection, the cells were washed with 2 ml of phosphate-buffered saline and incubated with 1 ml of Dulbecco's modified Eagle's medium without methionine/cysteine containing 10% dialyzed calf serum, 10 mM NH₄Cl, and 0.2 mCi of [³⁵S]methionine/cysteine mixture (Amersham Pharmacia Biotech, Pro-mix [³⁵S]). Four hours later, 0.5 ml of medium containing methionine/cysteine, 10% calf serum, and 10 mM HN₄Cl was added, and the cells were incubated for an additional 4 h at 37 °C. The medium was collected and the DNase I immunoprecipitated as above. One-half of the washed protein A-agarose beads were used for SDS-PAGE. The immunoprecipitated proteins were eluted in 50 µl of 0.1 M Tris-HCl, pH 7.0, 2% SDS, 10% 2-mercaptoethanol, and 20% glycerol by boiling for 5 min. The eluates were subjected to SDS-PAGE using 13% acrylamide SDS gels. Following electrophoresis, the gels were rinsed with 7% acetic acid/10% methanol for 15 min, soaked in Amplify solution (Amersham Pharmacia Biotech) for 15 min, and dried. Autoradiography was then carried out, and the intensity of the bands corresponding to DNase I was quantitated with a scanning densitometer (Molecular Dynamics). The other half of the beads were used to assay DNase I activity following the procedure of Sinicropi et al. (15). Briefly, 500 µl of the reaction mixture was added to the beads and incubated for 4 h at 37 °C. The units of DNase I activity were determined by comparing the values to those obtained with a standardized vial of DNase I (Sigma). Cells transfected with an empty plasmid served as control. The specific activity was obtained by dividing the units of activity by the relative intensity units obtained by scanning the autoradiograms.

	RESULTS

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Basal Phosphorylation of Murine and Bovine DNase I-- The phosphorylation of the two Asn-linked oligosaccharides of bovine and murine DNase I secreted by COS-1 cells is shown in Table I. The bovine DNase I had 12.6% of its oligosaccharides phosphorylated, mostly with a single Man-6-P residue, whereas 20.9% of the oligosaccharides on the murine enzyme were phosphorylated, including 7.7% with two Man-6-P residues. Whereas the murine DNase I is clearly better phosphorylated than the bovine enzyme, its level of phosphorylation is much less than that achieved by a bovine enzyme containing a R27K/N74K double mutation (Table I). Because the murine enzyme contains lysine residues at both of these positions (Fig. 1), it was surprising that it was phosphorylated so much less than the mutant bovine enzyme. We considered two possibilities to explain this difference. The first is that the murine DNase I, but not the bovine enzyme, contains amino acids that impair the interaction with phosphotransferase, thereby decreasing the level of phosphorylation. Alternatively, the bovine enzyme could have amino acids that are required for optimal phosphorylation that are absent in the murine form of the enzyme. The difference in the level of phosphorylation of the two forms of the enzyme could also result from both of these mechanisms being operative. The subsequent experiments were designed to test these possibilities.

View larger version (30K): [in this window] [in a new window]   Fig. 1.   Alignment of amino acid sequences of bovine and murine DNase I. The numbers refer to the sequence of the mature enzyme minus the signal sequence. N-linked glycosylation sites are at positions 18 and 106.

Phosphorylation of Bovine-Murine DNase I Chimeras-- We first constructed a series of plasmids encoding various chimeras between the R27K/N74K mutant bovine enzyme and the murine enzyme. The R27K/N74K double mutant was used so that all the chimeras had these two stimulatory amino acids. These chimeras were expressed in COS-1 cells and their level of oligosaccharide phosphorylation determined. The results of these experiments are summarized in Fig. 2. It is apparent that when murine sequences derived from either the amino, middle, or carboxyl regions of the protein were substituted into the bovine sequence, a lower level of phosphorylation resulted (compare CP1, CP2, and CP3 to bovine DNase I, Fig. 2). When additional murine sequences were introduced, the extent of phosphorylation decreased further (CP4-CP8). These results indicated that multiple amino acids distributed throughout the linear sequence of murine DNase I influenced the level of phosphorylation.

View larger version (28K): [in this window] [in a new window]   Fig. 2.   Oligosaccharide phosphorylation of bovine-murine chimeras. The various constructs tested are shown schematically, with the open bars representing murine DNase I sequence and the solid lines representing bovine DNase I sequence. The asterisks indicate the presence of lysine residues at positions 27 and 74 of the bovine sequence. The various constructs were expressed in COS-1 cells, and the extent of oligosaccharide phosphorylation of the secreted proteins determined as described under "Experimental Procedures." N refers to the number of separate determinations.

Effect of Point Mutations on the Phosphorylation of Murine DNase I-- In the next set of experiments, a number of amino acids in the murine DNase I sequence that differed from those found in the bovine sequence were replaced by the equivalent bovine residues. These were selected on the basis that the two corresponding amino acids differed significantly in structure. The effect of the amino acid substitution on oligosaccharide phosphorylation was then determined using the COS-1 cell expression system. As shown in Fig. 3, six mutations (V23S, E54Y, D61N, K117A, E124K, and P190S) resulted in 30% or more enhanced phosphorylation, whereas four mutations (R64H, I191T, V204A, and H208N) had no significant effect on the level of phosphorylation. Interestingly, the V23S mutation primarily enhanced the formation of oligosaccharides with one phosphate, whereas the E54Y and P190S substitutions stimulated the synthesis of oligosaccharides with two phosphates.

View larger version (34K): [in this window] [in a new window]   Fig. 3.   Effect of single amino acid substitutions on oligosaccharide phosphorylation of murine DNase I. Constructs were prepared that replaced specific amino acids in murine DNase I with the equivalent residue present in bovine DNase I or with an alanine. The various constructs were expressed in COS-1 cells, and the extent of phosphorylation of the secreted proteins was determined as described under "Experimental Procedures." The number of separate determinations is indicated in parentheses.

The substitutions that enhanced phosphorylation could be doing so by the removal of an inhibitory amino acid or the addition of a stimulatory amino acid. To distinguish between these possibilities, Val²³, Glu⁵⁴, and Pro¹⁹⁰ were replaced with alanine residues (the V23A, E54A, and P190A constructs) and analyzed for the level of phosphorylation (Fig. 3). The E54A substitution did not enhance phosphorylation suggesting that Tyr⁵⁴ is a stimulatory residue. The V23A construct was phosphorylated to the same extent as the V23S mutant, indicating that Val²³ inhibits phosphorylation. The P190A construct was phosphorylated somewhat better than wild type murine DNase I, although not nearly as well as the P190S construct. This suggests that Pro¹⁹⁰ may impair phosphorylation to a small extent, whereas Ser¹⁹⁰ has a stimulatory effect as well. Because Lys¹²⁴ has been shown to be a stimulatory residue in bovine DNase I (1), we assume that the enhanced phosphorylation obtained with the E124K substitution is due to the stimulatory effect of the lysine at this position. In addition, the K117A construct showed a markedly enhanced phosphorylation, consistent with Lys¹¹⁷ being an inhibitory residue in the context of the murine sequence, just as it is in the bovine sequence (1).

Effect of Multiple Mutations on Oligosaccharide Phosphorylation-- The effect of combinations of mutations on DNase I oligosaccharide phosphorylation is shown in Table II. Construct 1, which has four mutations (V23S, E54Y, E124K, and P190S) was phosphorylated 70.1% compared with 18.9% for the wild type murine DNase I analyzed in the same experiment. Thus mutation of these four residues brings the level of phosphorylation of the murine DNase I close to that obtained with the bovine enzyme containing the R27K/N74K mutations (79.2%). The extent of phosphorylation increased to 84.2% when a fifth residue (Lys¹¹⁷) was mutated to alanine (construct 2).

Effect of Mutations on Phosphorylation of the Oligosaccharide at Asn¹⁸-- Murine DNase I, like its bovine counterpart, contains two Asn-linked glycosylation sites, one at position 18 and the other at position 106 (Fig. 1). In our previous study of bovine DNase I, we established that the oligosaccharide at Asn¹⁸ primarily acquired a single Man-6-P residue, whereas the oligosaccharide at Asn¹⁰⁶ mostly acquired two Man-6-P residues (1). Furthermore, we showed that a R27K substitution selectively stimulated the phosphorylation of the oligosaccharide at position 18. Because Val²³ is positioned nearby Lys²⁷, and its substitution with either serine or alanine resulted in a selective increase in oligosaccharides with one Man-6-P residue, we postulated that Val²³ was impairing the phosphorylation of the Asn¹⁸ oligosaccharide. To pursue this, a construct was prepared in which the Asn¹⁰⁶ glycosylation site was deleted (construct 106). The Val²³ residue in this construct was then mutated to either a serine or an alanine (the V23S 106 and V23A 106 constructs). In this way, the effect of the Val²³ mutations on the phosphorylation of the oligosaccharide at Asn¹⁸ could be directly examined. As shown in Fig. 4, substitution of Val²³ with either an alanine or a serine residue doubled the phosphorylation of the oligosaccharide at position 18. Almost all of this increase was in oligosaccharides with a single Man-6-P residue. Because the V23A and V23S mutations in the wild type murine DNase I (with both oligosaccharides intact) result in a 26 and a 31% increase in total phosphorylation, respectively, it is likely that the inhibitory effect of Val²³ is selective for the oligosaccharide at position 18. 

View larger version (27K): [in this window] [in a new window]   Fig. 4.   Effect of amino acid substitutions on phosphorylation of the Asn18 oligosaccharide. Constructs were prepared with Asn106 changed to a Gln, and the other mutations were made as noted. The various constructs were expressed in COS-1 cells, and the extent of phosphorylation of the secreted proteins was determined as described under "Experimental Procedures." The number of separate determinations is indicated in parentheses.

The murine and bovine DNase I sequences also differ at positions 22 and 30, which are in the vicinity of Lys²⁷. Therefore, two additional constructs were prepared in which the bovine residue was substituted for the murine residue in the 106 context (constructs S22A 106 and S30R 106). When expressed in COS-1 cells, neither mutation resulted in enhanced phosphorylation of the oligosaccharide at position 18 (Fig. 4). Thus, of the three nonconservative differences in the amino-terminal 34 residues of bovine and murine DNase I, only Val²³ impairs phosphorylation (Fig. 1).

Effect of Mutations on Phosphorylation of the Oligosaccharide at Asn¹⁰⁶-- The E54Y, E124K, and P190S single mutations resulted in a marked increase in oligosaccharides with two Man-6-P residues, consistent with a selective stimulation of the phosphorylation of the Asn¹⁰⁶ oligosaccharide. To test this directly, a construct was prepared in which the Asn¹⁸ glycosylation site was deleted (construct 18). Residues 54, 124, and 190 were then mutated in various combinations, and the different constructs were expressed in COS cells and analyzed for the extent and type of phosphorylation (Table II). The murine 18 DNase I was phosphorylated 36.6%, with slightly more than half of the phosphorylated oligosaccharides containing two Man-6-P residues. Substitution of Glu¹²⁴ with a lysine increased the level of phosphorylation to 45.9% (construct 3), whereas substitution of Glu⁵⁴ with a tyrosine resulted in 60.7% phosphorylation (construct 4). Construct 5, which has two mutations (E54Y and P190S), was phosphorylated 72.4% whereas construct 6 (E54Y, E124K, and P190S) was 90.9% phosphorylated. It is also apparent that the proportion of oligosaccharides with two Man-6-P residues increased along with the extent of phosphorylation. Thus, mutation of only three residues results in almost complete phosphorylation of the oligosaccharide at Asn¹⁰⁶.

In a complementary experiment, Tyr⁵⁴ and Ser¹⁹⁰ were mutated to alanines in a bovine DNase I construct containing Lys⁷⁴ and only Asn¹⁰⁶ (Bwt (18, Lys⁷⁴)). As shown in Table II, mutation of Tyr⁵⁴ to alanine caused phosphorylation at Asn¹⁰⁶ to decrease from 85.9 to 62% (construct 7). Mutation of both Tyr⁵⁴ and Ser¹⁹⁰ to alanines caused a further decline in the level of phosphorylation to 23.1% (construct 8). These mutations also resulted in a decrease in the proportion of oligosaccharides with two Man-6-P residues, from 92.4 to 60%.

A Hydrophobic Residue Is Required at Position 54-- Our initial experiments using single amino acid changes showed that substitution of Glu⁵⁴ with tyrosine stimulated phosphorylation, whereas substitution with alanine had no effect (Fig. 2). To determine whether other bulky hydrophobic residues could substitute for tyrosine, constructs were prepared with Glu⁵⁴ changed to either phenylalanine or methionine. Both residues stimulated phosphorylation at the Asn¹⁰⁶ site, with methionine being equivalent to tyrosine and phenylalanine being somewhat less effective (Table III). These findings suggest that it is the hydrophobic character of the residue at position 54 that is most essential for stimulating phosphorylation at Asn¹⁰⁶.

Mouse DNase I Mutants Retain Enzymatic Activity-- Because phosphotransferase binds to a conformation-dependent protein determinant present on its substrates, the various amino acid substitutions could influence the level of phosphorylation by altering the proper folding of the DNase I. One indication of proper folding is the retention of enzymatic activity. Therefore, we expressed seven of the constructs in COS cells, collected the secreted protein, and determined the specific activity relative to that of the wild type enzyme. As shown in Fig. 5, the proteins encoded by four of the constructs exhibited endonuclease activity equal to or greater than that observed with the wild type enzyme. The construct with four mutations (V23A, E54Y, K117A, and P190A) has the lowest activity (55% of wild type level), but this protein was extremely well phosphorylated (65.2%), indicating that it is an excellent substrate for phosphotransferase. The two other constructs with small decreases in enzymatic activity (K117A and 106V23S) were also well phosphorylated. These data indicate that DNase I can tolerate many amino acid substitutions without major impairment of its endonuclease activity. This is consistent with the findings of Lazarus and co-workers (16, 17), who have reported the preparation of numerous mutants of human DNase I that have wild type or enhanced endonuclease activity.

View larger version (32K): [in this window] [in a new window]   Fig. 5.   Effect of amino acid substitutions on enzymatic activity. COS-1 cells transfected with wild type murine DNaseI and various mutants were labeled with [35S]methionine/cysteine for 4 h, and then the medium was collected after another 4 h. The DNase I in the medium was immunoprecipitated, and one-half of the material was analyzed by SDS-PAGE. The other half was used for determination of DNase I activity. S.A. refers to the specific activity of each construct. 2 is another designation for 106, i.e. missing the oligosaccharide at Asn106, which accounts for the increased mobility of these constructs on SDS-PAGE. Note that there is an excellent correlation between good phosphorylation of a construct and reduced mobility on SDS-PAGE.

	DISCUSSION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Our previous study of bovine DNase I identified four lysine residues (Lys²⁷, Lys⁵⁰, Lys⁷⁴, and Lys¹²⁴) that determine high level phosphorylation (79%) by phosphotransferase (1). Among these residues, Lys²⁷ and Lys⁷⁴ were most important, with Lys²⁷ causing the selective phosphorylation of the oligosaccharide at Asn¹⁸ and Lys⁷⁴ causing the selective phosphorylation of the oligosaccharide at Asn¹⁰⁸. The current study was stimulated by the observation that murine DNase I is phosphorylated only 20.9% despite having Lys²⁷ and Lys⁷⁴ as well as Lys⁵⁰. We reasoned that murine DNase I either lacked other residues present in the bovine enzyme that are essential for high level phosphorylation or contained inhibitory residues not found in the bovine species. Our experiments show that both postulates are correct. Furthermore, these data provide new insight into the nature of the phosphotransferase recognition domain.

Starting with the analysis of chimeric proteins containing elements of both bovine and murine DNase I and then proceeding to amino acid substitution experiments, we have identified four residues at positions 23, 54, 124, and 190 that account for the majority of the difference in the phosphorylation of the two forms of DNase I. The valine in position 23 of the murine sequence impairs phosphorylation because its replacement with either an alanine or a serine (as in the bovine species) enhances phosphorylation about 30%. Replacement of the glutamic acid at position 124 of the murine sequence with a lysine stimulated phosphorylation to about the same extent. This was expected because this residue was known to facilitate phosphorylation of the bovine DNase I. The unexpected and most dramatic effects were observed when the glutamic acid at position 54 and the proline at position 190 of the murine sequence were replaced with tyrosine and serine, respectively. Each of these substitutions stimulated phosphorylation about 75%. Because substitution of alanines at these positions had little (position 190) or no (position 54) effect, we conclude that Tyr⁵⁴ and Ser¹⁹⁰ function as positive elements. When these four changes were combined the resultant murine DNase I was phosphorylated 70%, close to the 79% value obtained with bovine DNase I containing Lys²⁷ and Lys⁷⁴. These values are equivalent to that observed with authentic lysosomal acid hydrolases.

The experiments using a mutant DNase I with the Asn¹⁰⁶ glycosylation site deleted demonstrated that the effect of Val²³ is primarily on the phosphorylation of the oligosaccharide at Asn¹⁸. As shown in the space filling model of DNase I (Fig. 6), Val²³ is located close to Asn¹⁸ and is adjacent to Lys²⁷, which is a key residue in the phosphorylation of the oligosaccharide attached to Asn¹⁸. Thus, we predict that Val²³ inhibits the interaction of Lys²⁷ with phosphotransferase.

View larger version (32K): [in this window] [in a new window]   Fig. 6.   Space filling model of bovine DNase I. Two views are shown to illustrate the locations of Asn18 and Asn106 (yellow): the stimulatory amino acids Lys27, Lys50, Lys74, Lys124, Tyr54, and Pro(Ser)190, and the inhibitory amino acids Val23 and Lys117. The core portion of the oligosaccharide at Asn18 is visible. This model was developed by Suck et al. (18, 19).

In contrast to Val²³, the residues Tyr⁵⁴, Lys¹²⁴, and Ser¹⁹⁰ function primarily to facilitate the phosphorylation of the oligosaccharide attached to Asn¹⁰⁶. Substitution of these three residues increased phosphorylation from 36.6 to 90.9% at this site. In addition, there was a strong enhancement of the formation of oligosaccharides with two Man-6-P residues. The importance of Tyr⁵⁴ and Ser¹⁹⁰ for phosphorylation at the Asn¹⁰⁶ site was confirmed by the finding that replacement of these residues with alanines in bovine DNase I resulted in a decline in phosphorylation from 85.9 to 23.1%. These results provide additional evidence that amino acids other than lysines are involved in the generation of the phosphotransferase recognition domain (5, 8, 11). It is apparent from Fig. 6 that Tyr⁵⁴ and Pro¹⁹⁰ are located a considerable distance from each other and from Asn¹⁰⁶. Tyr⁵⁴ is adjacent to Lys⁵⁰, which has also been shown to stimulate phosphorylation of the oligosaccharide at Asn¹⁰⁶ (1), so these two residues may act together.

When these results with murine DNase I are compared with those previously obtained with bovine DNase I, a number of interesting differences emerge with regard to their phosphotransferase recognition domains. The bovine DNase I is missing the two most potent stimulatory residues (Lys²⁷ and Lys⁷⁴) but also lacks the Val²³ inhibitory residue. Murine DNase I, on the other hand, has Lys²⁷ and Lys⁷⁴, but it also contains Val²³ and is missing the stimulatory residues Tyr⁵⁴, Lys¹²⁴, and Ser¹⁹⁰. Both species have the stimulatory residue Lys⁵⁰, as well as the inhibitory residue Lys¹¹⁷. The net result is that both bovine and murine DNase I are relatively poor substrates for phosphotransferase, but each can be converted into an excellent substrate by changing just a few amino acids. It is curious that these two forms of DNase I should differ so much in the manner in which they attenuate oligosaccharide phosphorylation by phosphotransferase. It is also interesting to speculate whether other secretory glycoproteins might contain potential phosphotransferase recognition elements that are either blocked by strategically located inhibitory amino acids or lack key stimulatory residues. This may have been a mechanism for controlling the compartmentalization of such proteins during evolution.

The finding that the optimal phosphorylation of DNase I requires widely spaced residues is consistent with our previous studies of cathepsin D (6-8), as well as the work of others with arylsulfatase A (20). We found that the minimal components of a phosphotransferase recognition patch in the carboxyl lobe of cathepsin D consisted of two noncontinuous primary sequences (Lys²⁰³ and amino acids 265-293) that come together in three-dimensional space. However, phosphorylation was enhanced by the presence of other regions of cathepsin D, including amino lobe elements, indicating that the optimal recognition domain involves a larger surface area. Consistent with these findings, Lukong et al. (21) recently reported that peptides derived from several regions of cathepsin D, including one located on the side opposite to the phosphorylated oligosaccharide, inhibit lysosomal enzyme phosphorylation by phosphotransferase. Schierau et al. (20) came to a similar conclusion using a totally different approach (20). These investigators prepared monoclonal antibodies that bind to at least six different epitopes at different locations on the surface of arylsulfatase A. All of the antibodies were shown to bind outside the lysine-rich recognition domain, yet Fab fragments of these antibodies inhibited interaction of arylsulfatase A with phosphotransferase. The authors concluded that their results support a model in which phosphotransferase interacts with a large surface area of arylsulfatase A. In the lysosomal enzyme aspartylglucosaminidase, three lysine residues and a tyrosine located in three spatially distinct regions of the structure have been found to be necessary for full phosphorylation (11). Mutagenesis of these residues indicates that they function in a cooperative manner, with all three sites being required for optimal phosphorylation.

If one accepts that phosphotransferase interacts with a large surface area on its target glycoproteins, it is still necessary to explain the striking finding that Lys²⁷ and Lys⁷⁴ selectively stimulate the phosphorylation of different oligosaccharides on DNase I. We have noted previously that Lys²⁷ and Lys⁷⁴ are located relatively close to the oligosaccharide the phosphorylation of which they affect and a considerable distance from the other oligosaccharide (1). Based on this fact, we suggested that these lysines serve to orient phosphotransferase in a manner that facilitates the phosphorylation of the oligosaccharide in the vicinity of the critical lysine. In the context of the current discussion, we would envisage phosphotransferase interacting with a broad surface of the protein with key lysines serving to orient the catalytic site toward the oligosaccharide.

Sahagian and co-workers (22) have suggested a simpler model for the recognition signal. They have proposed that the signal consists of just two lysine residues spaced about 34 Å apart with a particular orientation relative to the oligosaccharide. They also suggest that each lysine may be situated in specific microenvironments. This proposal is based on the finding that two lysines, spaced 34 Å apart, are necessary for full phosphorylation of cathepsin L and cathepsin D. However, in the case of cathepsin D, mutation of both lysines (Lys²⁰³ and Lys²⁹³) to alanines inhibits phosphorylation by only 70%. Because 30% of normal phosphorylation remains, other residues must be involved, consistent with the proposal that a large surface area is required for the generation of an optimal recognition determinant.

Phosphotransferase has been shown to be a very large 540-kDa complex composed of disulfide-linked homodimers of 166 and 51 kDa subunits and two 56-kDa subunits (23). As such, it should have the capability to contact a large surface area on its glycoprotein substrates. The ultimate explanation for how phosphotransferase interacts with its substrate may only come with the determination of the tertiary structure of this enzyme bound to its substrate.

	FOOTNOTES

^* This research was supported in part by United States Public Health Service Grant CA 08759, a grant from Genentech Inc., and a grant to Washington University from the Howard Hughes Medical Institute through the Undergraduate Biological Sciences Education Program.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Dept. of Medicine, Washington University School of Medicine, 660 S. Euclid Ave., Box 8125, St. Louis, MO 63110. Tel.: 314-362-8803; Fax: 314-362-8826.

	ABBREVIATIONS

The abbreviations used are: phosphotransferase, UDP-GlcNAc:lysosomal enzyme N-acetylglucosamine-1-phosphotransferase; Man-6-P, mannose 6-phosphate; PAGE, polyacrylamide gel electrophoresis.

	REFERENCES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

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