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J Biol Chem, Vol. 274, Issue 36, 25933-25944, September 3, 1999

Eosinophil Peroxidase Nitrates Protein Tyrosyl Residues
IMPLICATIONS FOR OXIDATIVE DAMAGE BY NITRATING INTERMEDIATES IN EOSINOPHILIC INFLAMMATORY DISORDERS^*

Weijia Wu^§, Yonghong Chen^§, and Stanley L. Hazen^§¶

From the Departments of  Cell Biology and ^¶ Cardiology, Cleveland Clinic Foundation, Cleveland, Ohio 44195 and the ^§ Chemistry Department, Cleveland State University, Cleveland, Ohio 63119

	ABSTRACT

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Eosinophil peroxidase (EPO) has been implicated in promoting oxidative tissue injury in conditions ranging from asthma and other allergic inflammatory disorders to cancer and parasitic/helminthic infections. Studies thus far on this unique peroxidase have primarily focused on its unusual substrate preference for bromide (Br) and the pseudohalide thiocyanate (SCN) forming potent hypohalous acids as cytotoxic oxidants. However, the ability of EPO to generate reactive nitrogen species has not yet been reported. We now demonstrate that EPO readily uses nitrite (NO₂), a major end-product of nitric oxide (^·NO) metabolism, as substrate to generate a reactive intermediate that nitrates protein tyrosyl residues in high yield. EPO-catalyzed nitration of tyrosine occurred more readily than bromination at neutral pH, plasma levels of halides, and pathophysiologically relevant concentrations of NO₂. Furthermore, EPO was significantly more effective than MPO at promoting tyrosine nitration in the presence of plasma levels of halides. Whereas recent studies suggest that MPO can also promote protein nitration through indirect oxidation of NO₂ with HOCl, we found no evidence that EPO can indirectly mediate protein nitration by a similar reaction between HOBr and NO₂. EPO-dependent nitration of tyrosine was modulated over a physiologically relevant range of SCN concentrations and was accompanied by formation of tyrosyl radical addition products (e.g. o,o'-dityrosine, pulcherosine, trityrosine). The potential role of specific antioxidants and nucleophilic scavengers on yields of tyrosine nitration and bromination by EPO are examined. Thus, EPO may contribute to nitrotyrosine formation in inflammatory conditions characterized by recruitment and activation of eosinophils.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Eosinophils play a central role in host defenses against a variety of cancers and both parasitic and helminthic infections (1-3). Increased levels of circulating and tissue eosinophils are also implicated in promoting cellular injury during asthma and other allergic inflammatory disorders (1-6). Eosinophils are thought to mediate many of their cytotoxic and tissue-destroying effects through their exceptional ability to generate oxidizing species (1-4, 7-9). Indeed, the respiratory burst of eosinophils generates several times as much superoxide (O₂) and hydrogen peroxide (H₂O₂) as a corresponding number of neutrophils (9, 10). Despite their known proclivity for producing reactive oxygen species and a wealth of clinical evidence linking eosinophils to host defenses and inflammatory tissue injury, structural identification of specific oxidation products formed by these leukocytes is lacking.

Eosinophil peroxidase (EPO),¹ an abundant heme protein secreted from activated eosinophils, plays a central role in oxidant production by eosinophils (1-4, 8, 11, 12). EPO amplifies the oxidizing potential of H₂O₂ produced during the respiratory burst by using it as a co-substrate to generate cytotoxic oxidants. Studies thus far have focused on the unusual substrate preference of EPO for physiological levels of bromide (Br) (8, 13-17) and the pseudohalide thiocyanate (SCN) (18-20), even in the presence of a vast molar excess of chloride (Cl), as is seen in vivo. Studies with proteins incubated in the presence of radioactive Br or SCN salts and either activated eosinophils or the EPO-H₂O₂ system have demonstrated covalent incorporation of radiolabel into target proteins (13, 14). However, structural identification of the oxidation products formed on proteins following exposure to EPO-generated oxidants is essentially unexplored. We recently identified 3-bromotyrosine and 3,5-dibromotyrosine as major products of protein oxidation by the EPO-H₂O₂-Br system (21). The role of brominating oxidants in promoting tissue injury in eosinophilic inflammatory disorders has not yet been established.

Another potential pathway for oxidative tissue damage by eosinophils may involve formation of nitrating intermediates by EPO. Immunohistochemical studies using antibodies raised against nitrotyrosine, a global marker for protein damage by reactive nitrogen species, intensely stain eosinophils present in the inflamed lung tissues of asthmatics (22). Klebanoff (23) demonstrated that the antimicrobicidal properties of eosinophil peroxidase-H₂O₂ systems are enhanced in the presence of nitrite (NO₂), the autoxidation product of ^·NO. Moreover, recent studies have shown that myeloperoxidase (MPO), a neutrophil- and monocyte-specific peroxidase, can use NO₂ and H₂O₂ as substrates to generate a reactive intermediate capable of nitrating phenolic compounds and protein tyrosyl residues (25-28). However, MPO and EPO are distinct gene products with divergent physical properties. Although both peroxidases contain heme prosthetic groups, structural studies have established that MPO contains a six-coordinate, high spin chlorin, while EPO possesses a high spin, six-coordinate protoporphyrin (24). Moreover, although human MPO is tetrameric and comprised of two heavy and two light chains, EPO is dimeric, having only one heavy and one light chain (7, 24). Whether these structural differences underlie the distinct halide and substrate specificities observed between MPO and EPO remains unknown. Furthermore, although increased levels of NO₂ and eosinophils have been reported in a variety of conditions, the ability of EPO to generate nitrating intermediates has not yet been explored.

The biological roles and potential targets of mammalian peroxidases are defined by their unique and often nonoverlapping substrate preferences (29, 30). We therefore sought to determine whether the EPO-H₂O₂ system of eosinophils could promote protein oxidative damage through formation of reactive nitrogen species. We now show that isolated EPO readily uses NO₂ as substrate to generate a reactive intermediate that nitrates protein tyrosyl residues in high yield. We demonstrate that EPO is more efficient than MPO at nitrating tyrosine and that EPO-dependent protein nitration is a preferred activity of the enzyme. Finally, we provide evidence that EPO also generates tyrosyl radical and that tyrosine nitration by EPO may involve a tyrosyl radical intermediate.

	EXPERIMENTAL PROCEDURES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Materials-- Organic solvents, H₃PO₄, NaH₂PO₄, Na₂HPO₄, and H₂O₂ were obtained from Fisher. Methane sulfonic acid and bromine were purchased from Fluka Chemical Co. (Ronkonkoma, NY). Constant boiling HCl was obtained from Pierce. L-[¹³C₆]Tyrosine and L-[²H₄]tyrosine were purchased from Cambridge Isotopes, Inc. (Andover, MA). All other reagents were purchased from Sigma unless otherwise indicated.

Isolation and Characterization of EPO and MPO-- Porcine EPO was isolated according to the method of Jorg (31) employing guaiacol oxidation as the assay (32). Purity of EPO preparations was assured before use by demonstrating a RZ of >0.9 (A₄₁₅/A₂₈₀), SDS-polyacrylamide gel electrophoresis analysis with Coomassie Blue staining, and in-gel tetramethylbenzidine peroxidase staining to confirm no contaminating MPO activity (33). MPO was initially purified from detergent extracts of human leukocytes by sequential lectin affinity and gel filtration chromatography as described (34). Trace levels of contaminating EPO were then removed by passage over a CM-52 ion exchange column (20). Purity of isolated MPO was established by demonstrating a RZ of 0.87 (A₄₃₀/A₂₈₀), SDS-polyacrylamide gel electrophoresis analysis with Coomassie Blue staining, and in-gel tetramethylbenzidine peroxidase staining to confirm no contaminating EPO activity (33). Enzyme concentrations were determined spectrophotometrically utilizing extinction coefficients of 89,000 and 112,000 M¹ cm¹/heme of MPO (35) and EPO (36, 37), respectively. The concentration of the MPO dimer was calculated as half of the indicated concentration of hemelike chromophore.

General Procedures-- SDS-polyacrylamide gel electrophoresis analysis was performed as described by Laemmli (38). Protein content was measured by the Markwell-modified Lowry assay with bovine serum albumin (BSA) as the standard (39). HOBr free of Br and bromate was prepared from liquid bromine the day of use as described (40). HOBr was quantified spectrophotometrically (₃₃₁ = 315 M¹ cm¹) (41) as its conjugate base, hypobromite (OBr), immediately prior to use. The concentration of reagent H₂O₂ (₂₄₀ = 39.4 M¹ cm¹) (42) was determined spectrophotometrically. Production of H₂O₂ by the glucose/glucose oxidase system was determined by oxidation of Fe(II) and formation of a Fe(III)-thiocyanate complex (25). Western blot analysis of nitrated proteins was determined as described using immunopurified rabbit polyclonal antibody against nitrotyrosine (Upstate Biotechnology, Inc., Lake Placid, NY) (43). The specificity of the primary antibody was confirmed by blocking immunoreactivity in coincubations with either 10 mM nitrotyrosine or 1 mM of the tripeptide Gly-nitro-Tyr-Ala as described (43). o,o'-Dityrosine, pulcherosine, trityrosine, and isodityrosine were prepared and quantified as described previously (44). 3-Bromotyrosine and 3,5-dibromotyrosine standards were synthesized from L-tyrosine and isolated by preparative HPLC as recently described (21). [¹³C₆]Ring-labeled analogs of 3-bromotyrosine and 3,5-dibromotyrosine were similarly prepared using L-[¹³C₆]tyrosine as starting material. UV-visible studies on authentic and EPO-generated 3-nitrotyrosine were performed on a  Bio Spectrophotometer (Perkin-Elmer). 3-Nitro-[¹³C₆]tyrosine, 3-chloro-[¹³C₆]tyrosine, and o, o'-[¹³C₁₂]dityrosine standards for gas chromatography-mass spectrometry (GC/MS) identification of products were synthesized and quantified as described previously (45).

5,5'-Dithiobis(2-nitrobenzoic acid) (DTNB) Formation by EPO-- 5-Thio-2-nitrobenzoic acid (TNB) was prepared fresh by reduction of 1 mM DTNB in 100 ml of sodium phosphate buffer (50 mM, pH 7.0) with 4 µl of 2-mercaptoethanol (46). EPO (14.2 nM) was then incubated at 37 °C in sodium phosphate buffer (50 mM, pH 7.0) with TNB (53 µM) in the absence or presence of the indicated concentrations of Br, SCN, NO₂, or Cl. Oxidation of TNB to DTNB was initiated by the addition of H₂O₂ (30 µM) and followed spectrophotometrically at 412 nm (₄₁₂ = 27,200 M¹ cm¹) (46).

Oxidation of Free L-Tyrosine and Protein-bound Tyrosyl Residues-- Unless otherwise specified, reactions were initiated by the addition of oxidant (H₂O_2, HOBr or glucose to the glucose/glucose oxidase system) and performed in sodium phosphate buffer (20 mM, pH 7.0) at 37 °C for 60 min under the conditions described in the figure and table legends. Reactions were stopped by the addition of phenol (1% final) and catalase (30 nM; for glucose/glucose oxidase studies) to the reaction mixture. The pH dependence of 3-nitrotyrosine formation was assayed in phosphate buffer (50 mM final) composed of mixtures of phosphoric acid and monobasic and dibasic sodium phosphate. The pH of each reaction mixture was determined at the end of the incubation and did not change by more than 0.2 pH units over the course of the reaction. Experiments utilizing the glucose/glucose oxidase system for H₂O₂ generation were performed in the presence of 100 µg/ml glucose and 20 ng/ml glucose oxidase (grade II; Roche Molecular Biochemicals) at 37 °C for the indicated times. Preliminary studies demonstrated that under these conditions, a constant flux of H₂O₂ (0.18 µM/min) was generated.

Protein Hydrolysis-- Preliminary studies confirmed that removal of NO₂ prior to acidification of proteins was essential to avoid artifactual nitration of proteins. Proteins oxidized in vitro were therefore prepared for analysis by first precipitating and desalting them in a single-phase extraction mixture composed of H₂O/methanol/H₂O-saturated diethyl ether (1:3:7, v/v/v) as described (47). Proteins were typically hydrolyzed by incubating the desalted protein pellet with 6 N HCl (0.5 ml) supplemented with 1% phenol for 24 h (47). Prior to initiating hydrolysis, acid mixtures were degassed under vacuum and then sealed under a blanket of argon. When chlorotyrosine and/or bromotyrosine determinations were performed, formation of trace levels of halogenated tyrosine analogs during acid hydrolysis was avoided by hydrolysis in 4 N methane sulfonic acid (0.5 ml) supplemented with 1% phenol for 24 h at 100 °C under a blanket of argon (21). Control experiments utilizing proteins supplemented with L-[²H₄]tyrosine as internal standard added prior to acid hydrolysis and then analyzed by GC/MS (48) demonstrated no detectable intrapreparative formation of 3-nitro-[²H₃]tyrosine, 3-chloro-[²H₃]tyrosine, or 3-bromo-[²H₃]tyrosine under these conditions.

Reverse Phase HPLC Quantification of Tyrosine Oxidation Products-- Quantification of oxidation products from free L-tyrosine (3-nitrotyrosine, 3-bromotyrosine, 3,5-dibromotyrosine, 3-chlorotyrosine, and o,o'-dityrosine) was performed on a Beckman Gold HPLC system equipped with diode array and fluorescence detectors. Separations were performed on a C18 column (Beckman Ultrasphere, 5 µm, 4.6 × 250 mm) equilibrated with solvent A (0.1% trifluoroacetic acid, pH 2.5). L-Tyrosine and its oxidation products were eluted at a flow rate of 1 ml/min with a linear gradient generated with solvent B (0.1% trifluoroacetic acid in methanol, pH 2.5) as follows: 0% solvent B for 5 min, 0-100% solvent B over 30 min, 100% solvent B for 10 min. Products were quantified employing standard curves constructed with authentic synthetic standards. N-acetyl-L-tyrosine-derived oxidation products were isolated, and their structures were confirmed by on-line electrospray ionization mass spectrometric analysis as described below. Routine quantification of N-acetyl-L-tyrosine oxidation products was determined by reverse phase HPLC with UV detection (A₂₈₀) as above, utilizing external calibration curves constructed with known amounts of their nonacetylated counterparts (and assuming identical extinction coefficients). The retention times of N-acetyl-L-tyrosine analogs of tyrosine oxidation products were determined using authentic standards isolated at the time of on-line liquid chromatography/mass spectrometric analysis (LC/MS).

3-Nitrotyrosine, 3-bromotyrosine, 3,5-dibromotyrosine, o,o'-dityrosine, and 3-chlorotyrosine in protein hydrolysates were routinely quantified by reverse phase HPLC with electrochemical (coulometric) detection on an ESA (Cambridge, MA) CoulArray HPLC instrument equipped with UV detector and electrochemical cells (six channels) arranged in series and set to increasing specified potentials: channel 1 (300 mV); channel 2 (350 mV); channel 3 (480 mV); channel 4 (650 mV); channel 5 (800 mV); channel 6 (880 mV). Chromatographic separation of amino acids was performed on a a Projel TSK ODS-80 TM column (5 µm, 4.6 × 250 mm) equilibrated with mobile phase A (15 mM lithium phosphate, 3 mg/liter lithium dodecyl sulfate, pH 3.2). Products were eluted at a flow rate of 1 ml/min with a nonlinear gradient generated with mobile phase B (50% methanol, 15 mM lithium phosphate, 3 mg/liter lithium dodecyl sulfate, pH 3.2) as follows: isocratic elution at 0% mobile phase B for 10 min, 0-15% mobile phase B over 10 min, isocratic elution at 15% mobile phase B for 10 min, 15-20% mobile phase B over 10 min, isocratic elution at 20% mobile phase B for 10 min, 20-100% mobile phase B over 20 min, isocratic elution at 100% mobile phase B for 10 min. Peak identity was established by demonstrating the appropriate retention time, redox potential, and ratio of integrated currents in adjacent channels and by the method of standard additions for each analyte. L-Tyrosine, 3-nitrotyrosine, 3-bromotyrosine, 3,5-dibromotyrosine, o,o'-dityrosine, and 3-chlorotyrosine standards (0.5-50 pmol each on column) were also dissolved together and used to generate external calibration curves. The experimental error in data obtained by this assay is less than ±5%. Control experiments comparing quantitation of the tyrosine analogues by stable isotope dilution GC/MS (45) versus reverse phase with electrochemical detection demonstrated comparable results.

Mass Spectrometry-- LC/MS was performed using electrospray ionization (ESI) and detection with a Quatro II triple quadrupole mass spectrometer (Micromass, Inc.) interfaced with an HP 1100 high performance liquid chromatograph (Hewlett Packard, Wilmington, DE) equipped with diode array detector. L-Tyrosine oxidation products were resolved on an Ultrasphere C18 column (Beckman; 5 µm, 4.6 × 250 mm) at a flow rate of 1 ml/min and a linear gradient between H₂O (with 0.3% formic acid) and methanol (with 0.3% formic acid) over 30 min. Column eluent was split (970 µl/min to diode array, 30 µl/min to mass detector) and analyzed in the positive ion mode with a cone potential of 30 eV. GC/MS analyses of HPLC-isolated L-tyrosine oxidation products were performed following derivatization to their n-propyl perheptafluorylbutyryl or n-propyl perpentafluorylproprionyl derivatives (49). 3-Nitrotyrosine was also analyzed by GC/MS analysis as its n-propyl perheptafluorylbutyryl derivative following reduction to 3-aminotyrosine (50). Negative ion chemical ionization GC/MS studies were performed utilizing a Perkin Elmer (Norwalk, CT) TurboMass spectrometer equipped with chemical ionization probe.

	RESULTS

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

3-Nitrotyrosine and Tyrosyl Radical Addition Products Are Formed during L-Tyrosine Oxidation by the Eosinophil Peroxidase-H₂O₂-NO₂ System-- The addition of purified EPO and H₂O₂ to reaction buffer (20 mM sodium phosphate, 100 µM diethylenetriaminepentaacetic acid, pH 7.0) supplemented with L-tyrosine generated several new species with distinct retention times on reverse phase HPLC analysis with diode array detection (Fig. 1, upper panel, peaks a-c). The ultraviolet absorption maximum of each peak was nearly indistinguishable from L-tyrosine (_max 275-285 nm). LC/MS analyses were consistent with formation of the tyrosyl radical addition products o,o'-dityrosine and isodityrosine (peak a; m/z 361 (M + H)⁺), pulcherosine (peak b; m/z 540 (M + H)⁺), and trityrosine (peak c; m/z 540 (M + H)⁺) (44). Likewise, HPLC analysis with UV and fluorescence detection demonstrated co-migration with authentic synthetic standards prepared as described under "Experimental Procedures." Following the addition of NO₂, the reaction mixture developed an intense yellow color. Reverse phase HPLC analysis demonstrated the formation of an additional major (peak I) and minor (peak d) product (Fig. 1, bottom panel) as well as enhanced production of the tyrosyl radical addition products (peaks a-c). The UV-visible spectra of the new products (peaks I and d) both illustrated similar absorbance maxima in the visible wavelength range (_max 350-355 nm), consistent with nitration of the aromatic amino acid (51). Use of heat-inactivated EPO or omission of either L-tyrosine or H₂O₂ from the reaction mixture both resulted in no detectable production of the products. The reaction products formed (peak I and peaks a-d) were stable to treatment with acid (4 N HCl at 100 °C for 24 h), incubation at 37 °C with H₂O₂, and the addition of a molar excess of either reducing agents or nucleophilic scavengers (e.g. NaCNBH₃, methionine, 2-mercaptoethanol, taurine, ammonium acetate).

View larger version (16K): [in this window] [in a new window]   Fig. 1.   Reverse-phase HPLC separation of L-tyrosine oxidation products generated by eosinophil peroxidase. Top, L-Tyrosine (500 µM) was incubated with eosinophil peroxidase (57 nM) and H2O2 (500 µM) in sodium phosphate buffer (20 mM, pH 7.0) at 37 °C for 60 min. Products were subsequently analyzed by reverse phase HPLC as described under "Experimental Procedures." Bottom, L-tyrosine (500 µM) was incubated with eosinophil peroxidase (57 nM), H2O2 (500 µM), and NO2 (1 mM) in sodium phosphate buffer (20 mM, pH 7.0) at 37 °C for 60 min. Products were then analyzed by reverse phase HPLC as described under "Experimental Procedures."

To characterize the structures of the L-tyrosine oxidation products generated by the EPO-H₂O₂-NO₂ system, a variety of analytical techniques were employed. The pH dependence of the UV-visible absorbance spectrum of HPLC-isolated peak I is illustrated in Fig. 2 (upper panel) and is identical to that observed with authentic 3-nitrotyrosine. To confirm the structure of the major L-tyrosine oxidation product (peak I) as 3-nitrotyrosine, LC/MS analysis was performed. The retention time and positive ion mass spectrum of peak I was identical to that of authentic 3-nitrotyrosine and demonstrated a m/z of 227 (M + H)⁺ (Fig. 2, lower panel). LC/MS analysis of the minor products generated during oxidation of L-tyrosine by the complete EPO-H₂O₂-NO₂ system was consistent with the prior structural assignments of peaks a-c and suggested that peak d was composed of a nitrated analog of o,o'-dityrosine (e.g. 3-nitro-o,o'-dityrosine, m/z 406 (M + H)⁺). These results, combined with the chemical stability and fluorescence spectra (data not shown) of the compounds formed, suggest that the major compounds generated during oxidation of free L-tyrosine by EPO and H₂O₂ are tyrosyl radical addition products. In contrast, in the presence of EPO, H₂O₂, and NO₂, 3-nitrotyrosine is the major product formed.

View larger version (18K): [in this window] [in a new window]   Fig. 2.   UV-visible spectra and positive ion electrospray ionization mass spectrum of 3-nitrotyrosine generated by the EPO-H2O2-NO2 system. Top, the major L-tyrosine oxidation product generated by the EPO-H2O2-NO2 system (peak I, Fig. 1, bottom) was isolated by reverse phase HPLC as described under "Experimental Procedures." The isolated product was dried, resuspended at the indicated pH, and then analyzed by UV-visible spectroscopy (200-600 nm). Bottom, the major L-tyrosine oxidation product generated by the EPO-H2O2-NO2 system (peak I in Fig. 1, bottom) was analyzed by LC/MS as described under "Experimental Procedures." The positive ion ESI mass spectrum is shown. The structure and predicted m/z of protonated 3-nitrotyrosine is also shown (inset).

Characterization of Reaction Requirements for EPO-dependent Formation of 3-Nitrotyrosine-- The reaction requirements for EPO-catalyzed nitration of L-tyrosine yielding 3-nitrotyrosine are illustrated in Fig. 3. At pH 7.0 and 100 µM L-tyrosine and NO₂, 3-nitrotyrosine synthesis by EPO (following the bolus addition of 100 µM H₂O₂) was linear over the first 15 min and reached a plateau within 30 min (Fig. 3A). At higher L-tyrosine concentrations, the yield of 3-nitrotyrosine decreased, consistent with the competing bimolecular mechanism of o,o'-dityrosine formation. EPO-catalyzed nitration was optimal between pH 6 and 6.5, where approximately 0.35 mol of product was formed for each mole of oxidant consumed (Fig. 3B). A dose-dependent increase in the synthesis of 3-nitrotyrosine was observed over a (patho)physiologically relevant range of NO₂ concentrations (Fig. 3C). The H₂O₂ concentration dependence for 3-nitrotyrosine production by EPO yielded a similarly shaped plot and overall yield (Fig. 3D). At high concentrations of H₂O₂, however, the total amount of 3-nitrotyrosine formed decreased, consistent with either substrate inhibition or an interaction between H₂O₂ and an intermediate in 3-nitrotyrosine formation (data not shown).

View larger version (16K): [in this window] [in a new window]   Fig. 3.   Reaction characteristics of 3-nitrotyrosine formation by the eosinophil peroxidase. Reactions (0.5 ml) were performed at 37 °C for 60 min in sodium phosphate buffer (50 mM, pH 7.0) supplemented with L-tyrosine (100 µM), H2O2 (100 µM), NO2 (100 µM) and eosinophil peroxidase (57 nM) (unless otherwise indicated). Reactions were terminated by the addition of phenol (1%), and the extent of 3-nitrotyrosine formation was determined as described under "Experimental Procedures." Results represent the mean values from two or three independent experiments.

3-Nitrotyrosine Is a Preferred Product of Eosinophil Peroxidase at Physiologically Relevant Concentrations of L-Tyrosine, Halides, and NO₂-- Thiocyanate (SCN) and bromide (Br) are reported to be preferred substrates for EPO (13-16, 18). To estimate the relative substrate preferences of isolated EPO for NO₂ versus other reported substrates for the enzyme, we examined the initial rate of DTNB formation in reaction mixtures containing EPO, TNB, H₂O₂, and various concentrations of anionic substrates (SCN, Br, NO₂, Cl) at neutral pH. The relative rates of TNB oxidation observed by EPO were as follows: SCN > Br > NO₂ Cl (Fig. 4). Although EPO does not conform to Michaelis-Menten kinetics (e.g. high concentrations of H₂O₂ inhibit tyrosine nitration), a measure of the relative ability of EPO to discriminate in favor of a particular substrate in the presence of a mixture of competing substrates can be calculated as an apparent specificity constant (k_x) for each substrate (33). Because high levels of H₂O₂ can inhibit oxidant production, we estimated the kinetic parameters under conditions that minimize inhibition and mimic physiological conditions (e.g. neutral pH and low concentrations of H₂O₂) (Table I). SCN was the preferred substrate for EPO, demonstrating an approximately 2.7-fold higher apparent specificity constant than Br, the next best substrate examined. EPO preferred Br approximately 4-fold more than NO₂ (Table I). Implicit in this type of kinetic analysis is the assumption that the reactive nitrogen species formed by the EPO-H₂O₂-NO₂ system oxidize TNB at a rate and stoichiometry comparable with that of other hypohalous acids formed by the enzyme.

View larger version (15K): [in this window] [in a new window]   Fig. 4.   Eosinophil peroxidase-dependent oxidation of TNB in the presence of Br, NO2, Cl, or SCN. Eosinophil peroxidase (14.2 nM) was mixed with 53 µM TNB and the indicated concentrations of Br (), NO2 (), Cl(), or SCN (). Reactions were initiated by the addition of 30 µM H2O2, and DTNB formation was monitored spectrophotometrically at 412 nm as described under "Experimental Procedures." Rates of DTNB formation were corrected for TNB oxidation in the absence of anionic substrate (0.26 µM/min). Data represent the mean values for three independent experiments.

View this table: [in this window] [in a new window]   Table I Kinetic parameters for substrates for eosinophil peroxidase The apparent Vmax and Km values for chloride, nitrite, bromide, and thiocyanate were determined by linear regression analysis of double reciprocal plots for the rate of TNB formation versus anion concentration for experiments performed at neutral pH and 30 µM H2O2 as in Fig. 4. The catalytic rate constants (kobs) were calculated by dividing Vmax values by the concentration of EPO. The specificity constants (kx) were determined by dividing kobs by Km.

As an alternative means of assessing the potential significance of EPO-dependent tyrosine nitration, we performed product analyses of the major L-tyrosine oxidation products formed by the enzyme system (Table I). When plasma levels of L-tyrosine and halides were incubated with isolated EPO, H₂O₂, and concentrations of NO₂ as might be observed in inflammatory tissues or fluids (tyrosine = Br = NO₂ = 100 µM, Cl = 100 mM; Refs. 25 and 52-56), 3-nitrotyrosine was formed in high yield (Table II). In fact, 3-nitrotyrosine was the major product formed and was present at 2-3-fold greater levels than either 3-bromotyrosine or o,o'-dityrosine (Table II). Despite that vast molar excess of Cl, no detectable formation of 3-chlorotyrosine was observed under all of the conditions examined, consistent with the low apparent specificity constant observed (Tables I and II). The highest yields of 3-nitrotyrosine production were observed during incubations in the absence of Br, consistent with the unusual substrate preference of EPO for this halide.

View this table: [in this window] [in a new window]   Table II L-Tyrosine oxidation by eosinophil peroxidase in the presence of physiological concentrations of Cl, Br, and NO2 L-tyrosine (100 µM) was incubated with eosinophil peroxidase (57 nM), H2O2 (100 µM), and the indicated anionic substrates (Br = NO2 = 100 µM, Cl = 100 mM) in 1 ml of sodium phosphate buffer (20 mM, pH 7.0) at 37 °C for 60 min. The quantities of o,o'-dityrosine, 3-bromotyrosine, 3-nitrotyrosine, and 3-chlorotyrosine formed were then determined by reverse phase HPLC with UV detection as described under "Experimental Procedures." Results are the mean ± S.D. of three independent experiments. The limit of detection for these analyses is <0.1 nmol for each analyte examined.

3-Nitrotyrosine Formation by Eosinophil Peroxidase Occurs in the Presence of Nucleophilic Scavengers and Is Inhibited by Ascorbate and Urate-- Reactive oxidants and diffusible radical species generated by peroxidases in vivo will encounter a variety of nucleophilic scavengers and antioxidant compounds. To characterize the nature of the reactive halogenating and nitrating intermediates generated by EPO, we incubated the enzyme with H₂O₂, Br, and NO₂ under a variety of conditions and then quantified 3-bromotyrosine and 3-nitrotyrosine production. Again, when L-tyrosine was incubated with EPO, H₂O₂ (100 µM), and equivalent amounts of Br and NO₂ (100 µM each), 3-nitrotyrosine was the major oxidation product formed and was produced in 2-3-fold higher yield than 3-bromotyrosine (Fig. 5). The addition of methionine, a potent scavenger of reactive halogenating species, completely ablated 3-bromotyrosine production. In contrast, the addition of the thiol ether-containing amino acid to the reaction mixture had no significant effect on 3-nitrotyrosine production (Fig. 5). Likewise, the addition of reduced thiol compounds such as glutathione totally inhibited tyrosine bromination but only partially blocked aromatic nitration by the peroxidase. The addition of either ascorbic acid or uric acid potently inhibited both 3-bromotyrosine and 3-nitrotyrosine production. The addition of primary amine-containing species (e.g. N-acetyl lysine) to the reaction mixture did not attenuate either 3-bromotyrosine or 3-nitrotyrosine production. These results are consistent with our recent report that N-monobromamines serve as brominating intermediates for tyrosine (21). They also suggest that the nitrating intermediate formed by the EPO-H₂O₂-NO₂ system is not scavenged by amine groups. Finally, these results suggest that aromatic nitration reactions may be mediated by EPO in inflammatory fluids and tissues where NO₂ levels are increased and antioxidants such as ascorbate and urate may be depleted.

View larger version (16K): [in this window] [in a new window]   Fig. 5.   Effect of nucleophilic scavengers and antioxidants on the yield of eosinophil peroxidase-mediated nitration and bromination of L-tyrosine. L-Tyrosine (100 µM) was incubated with eosinophil peroxidase (57 nM), H2O2 (100 µM), NO2 (100 µM), Br (100 µM), and the indicated reagents (100 µM) in sodium phosphate buffer (20 mM, pH 7.0) at 37 °C for 60 min. The content of 3-nitrotyrosine and 3-bromotyrosine formed was then determined by reverse phase HPLC as described under "Experimental Procedures."

Eosinophil Peroxidase Nitrates Protein Tyrosyl Residues in High Yield-- The ability of EPO to nitrate protein tyrosyl residues was first examined by incubating a target protein (e.g. BSA) with the complete EPO-H₂O₂-NO₂ system and then performing SDS-polyacrylamide gel electrophoresis analysis with Western blot analysis using affinity-purified polyclonal antibodies specific for 3-nitrotyrosine (43). A NO₂-dependent increase in the intensity of staining was observed following exposure of the protein to the enzymatic system (Fig. 6). To confirm that 3-nitrotyrosine was produced in proteins exposed to the EPO-H₂O₂-NO₂ system, protein hydrolysates were directly analyzed for 3-nitrotyrosine production by GC/MS in the negative-ion chemical ionization mode employing synthetic 3-nitro-[¹³C₆]tyrosine as an internal standard, as described under "Experimental Procedures." Ions with the appropriate mass-to-charge ratio (m/z 464 (M) and 444 (M  HF)) and identical retention time to that observed with their corresponding stable isotope-labeled fragment ions were readily observed for the n-propyl-perheptafluorylbutyryl derivative of 3-nitrotyrosine. Moreover, reduction of the products prior to analysis by GC/MS demonstrated ions with identical retention time and appropriate m/z for that of the n-propyl perheptafluorylbutyryl derivative of 3-aminotyrosine (m/z 806 (M  HF), m/z 762 (M  HF  CO₂) and m/z 628 (M  heptafluorylbutyryl)). Collectively, these results unambiguously confirm that EPO catalyzes nitration of protein tyrosyl residues in the presence of the co-substrates, H₂O₂ and NO₂.

View larger version (23K): [in this window] [in a new window]   Fig. 6.   Nitration of protein tyrosine residues by eosinophil peroxidase. BSA (0.4 mg/ml) was incubated with eosinophil peroxidase (57 nM), H2O2 (100 µM), and the indicated concentrations of NO2 in sodium phosphate buffer (20 mM, pH 7.0) at 37 °C for 60 min. After incubation, 1.2 µg of protein was loaded on 10% SDS-polyacrylamide gels for electrophoresis, and proteins were transferred and immunostained using a rabbit polyclonal antibody against 3-nitrotyrosine (top) or stained with Coomassie Blue (bottom).

To characterize the significance of EPO-dependent protein nitration, the content of protein-bound 3-nitrotyrosine produced by the enzyme was determined by reverse phase HPLC with electrochemical detection as described under "Experimental Procedures." BSA was incubated with the complete EPO-H₂O₂-NO₂ system, while the concentrations of either NO₂ (Fig. 7, upper panel) or H₂O₂ (Fig. 7, lower panel) were varied. Nitration of protein tyrosyl residues occurred readily in a concentration-dependent manner for both co-substrates. The overall yield of EPO-catalyzed nitration of protein tyrosyl residues as a function of each substrate is shown in Fig. 8. The highest yields (10-20%) of protein nitration were noted when NO₂ concentrations were high and H₂O₂ concentrations were low. These results suggest that protein nitration would be highest under conditions where H₂O₂ production occurs at a low rate, similar to the conditions that are likely to be present in vivo.

View larger version (11K): [in this window] [in a new window]   Fig. 7.   Characterization of NO2 and H2O2 concentration dependences of eosinophil peroxidase-mediated nitration of protein tyrosyl residues. Top, BSA (0.4 mg/ml) was incubated with eosinophil peroxidase (57 nM), H2O2 (50 µM total, added in 10 µM increments at 10-min intervals), and the indicated concentrations of NO2 in sodium phosphate buffer (20 mM, pH 7.0) for 60 min at 37 °C. Oxidation products were then desalted and subjected to acid hydrolysis, and the content of 3-nitrotyrosine was determined by reverse phase HPLC with electrochemical detection as described under "Experimental Procedures." Bottom, BSA (0.4 mg/ml) was incubated with eosinophil peroxidase (57 nM), NaNO2 (100 µM), and the indicated concentrations of H2O2 (added in 10 µM increments at 10 min intervals) in sodium phosphate buffer (20 mM, pH 7.0) for 60 min at 37 °C. 3-Nitrotyrosine production was then determined as described under "Experimental Procedures." Data represent the mean ± S.D. of results from three independent experiments.

View larger version (11K): [in this window] [in a new window]   Fig. 8.   Overall yield of protein tyrosyl residue nitration by eosinophil peroxidase at physiological concentrations of NO2 and H2O2. The overall yield of EPO-dependent nitration of protein tyrosyl residues for the experiments described in Fig. 8 was calculated as the moles of 3-nitrotyrosine formed divided by the moles of oxidant (H2O2) added to the reaction mixture. Data represent the mean results from three independent experiments.

To more fully examine the potential physiological significance of protein nitration by EPO, we quantified the levels of formation of multiple tyrosyl residue oxidation products (3-nitrotyrosine, 3-bromotyrosine, 3-chlorotyrosine, and o,o'-dityrosine) in proteins incubated with isolated EPO, a hydrogen peroxide-generating system (glucose/glucose oxidase), and concentrations of halides and NO₂ as might be observed in inflammatory fluids (i.e. 100 mM Cl, 100 µM Br, and 100 µM NO₂). Again, EPO nitrated protein tyrosyl residues to a greater extent than that of all other tyrosine oxidation products examined (Table III). Under the conditions employed, only minimal amounts of o,o'-dityrosine and no detectable levels of 3-chlorotyrosine were formed (Table III). EPO-catalyzed bromination of protein tyrosyl residues occurred, albeit at a level approximately one-third that of tyrosine nitration. Generation of the tyrosine oxidation products demonstrated an absolute requirement for EPO and the H₂O₂-generating system. Collectively, these results demonstrate that EPO uses NO₂ as a preferred substrate to form a reactive nitrogen intermediate(s) even in the presence of physiological levels of halides.

View this table: [in this window] [in a new window]   Table III Oxidation of protein tyrosyl residues by eosinophil peroxidase in the presence of physiological concentrations of Cl, Br, and NO2 BSA (0.4 mg/ml) was incubated with eosinophil peroxidase (57 nM), glucose (100 µg/ml), glucose oxidase (20 ng/ml), and the indicated anionic substrates (Br = NO2 = 100 µM, Cl = 100 mM) in sodium phosphate buffer (20 mM, pH 7.0) at 37 °C for 4 h. Following reaction, protein was desalted and subjected to acid hydrolysis, and the content of 3-nitrotyrosine, 3-bromotyrosine, o,o'-dityrosine, and 3-chlorotyrosine was determined by reverse phase HPLC with electrochemical detection as described under "Experimental Procedures." Results are the mean ± S.D. of three independent experiments. The limit of detection for these analyses is <0.05 mmol/mol tyrosine for each analyte examined.

The pseudohalide SCN is a preferred substrate of EPO (18-20). We were therefore interested in examining the potential influence of SCN levels on EPO-catalyzed nitration. Extracellular fluid and tissue levels of this unusual anion have not been reported; however, plasma levels of SCN vary considerably (normal values are <69 µM) depending upon diet and smoking habits in normal individuals (57). In a separate set of experiments, either free L-tyrosine (Fig. 9, left) or BSA (Fig. 9, right) was incubated with NO₂ (100 µM) and plasma levels of halides (100 mM Cl and 100 µM Br) in the absence and presence of varying amounts of SCN, and the content of 3-nitrotyrosine was determined. EPO-mediated nitration was attenuated by the addition of SCN over the reported range of plasma levels of SCN (Fig. 9). In the presence of equivalent levels of NO₂ and SCN (100 µM each), 3-nitrotyrosine formation was significantly inhibited; however, nitration of both free and protein-bound tyrosine residues was still detectable.

View larger version (16K): [in this window] [in a new window]   Fig. 9.   Inhibitory effect of SCN on 3-nitrotyrosine production by eosinophil peroxidase. Left, L-tyrosine (100 µM) was incubated with eosinophil peroxidase (57 nM), H2O2 (100 µM), and NO2 (100 µM) in the presence of the indicated concentrations of NaSCN in sodium phosphate buffer (20 mM, pH 7.0) at 37 °C for 1 h. The content of 3-nitrotyrosine formed was then determined as described under "Experimental Procedures." Data represent the mean ± S.D. of results from three independent experiments. Right, BSA (0.4 mg/ml) was incubated with eosinophil peroxidase (57 nM), glucose (100 µg/ml), glucose oxidase (20 ng/ml), and NO2 (100 µM) in the presence of the indicated concentrations of NaSCN in sodium phosphate buffer (20 mM, pH 7.0) at 37 °C overnight. The content of protein-bound nitrotyrosine formed was then determined as described under "Experimental Procedures." Data represent the mean ± S.D. of results from three independent experiments.

Eosinophil Peroxidase Is More Effective Than Myeloperoxidase at Generating Nitrotyrosine in the Presence of NO₂, H₂O₂, and Plasma Levels of Halides-- Since MPO-dependent formation of nitrotyrosine may also contribute to protein nitration at sites of inflammation (25-28, 58, 59), we sought to compare the ability of isolated MPO and EPO to nitrate target proteins. BSA was incubated with equal concentrations of each peroxidase in the presence of plasma levels of halides (100 mM Cl and 100 µM Br) and an H₂O₂-generating system (glucose/glucose oxidase) to more closely mimic the low steady flux of peroxide that might be formed in vivo. Kinetic analyses comparing the ability of each peroxidase to use NO₂ as substrate for protein nitration demonstrated that EPO formed at least 4-fold more 3-nitrotyrosine on target proteins than MPO at all times examined (Fig. 10, left). Moreover, regardless of the concentration of NO₂ examined (0-100 µM), EPO was significantly more effective than MPO at nitrating protein tyrosyl residues (Fig. 10, right). Thus, at sites of eosinophilic inflammatory disorders, EPO may contribute to 3-nitrotyrosine formation.

View larger version (12K): [in this window] [in a new window]   Fig. 10.   Comparison of protein nitration by eosinophil peroxidase and myeloperoxidase. Left, BSA (0.4 mg/ml) was incubated with equivalent amounts (57 nM each) of either EPO or MPO, NaNO2 (100 µM), glucose (100 µg/ml), and glucose oxidase (20 ng/ml) in sodium phosphate buffer (50 mM, pH 7.0) supplemented with NaCl (100 mM) and NaBr (100 µM) at 37 °C for the indicated periods of time. Under these conditions, a constant flux of H2O2 (0.18 µM/min) is generated by the glucose/glucose oxidase system. Reactions were stopped by the addition of phenol (1% final concentration) and then immediate delipidation/desalting of proteins by a single phase extraction mixture as described under "Experimental Procedures." The content of 3-nitrotyrosine was then determined by reverse phase HPLC with electrochemical detection as described under "Experimental Procedures." Right, BSA (0.4 mg/ml) was incubated with 57 nM of either EPO or MPO, glucose (100 µg/ml), and glucose oxidase (20 ng/ml) in the presence of the indicated concentrations of NO2 in sodium phosphate buffer (50 mM, pH 7.0) supplemented with NaCl (100 mM) and NaBr (100 µM) for 12 h at 37 °C. The content of 3-nitrotyrosine was then determined by reverse phase HPLC with electrochemical detection. Data represent the mean ± S.D. of results from three independent experiments.

The Mechanism of Eosinophil Peroxidase-dependent Production of 3-Nitrotyrosine Involves Formation of a Tyrosyl Radical Intermediate-- To examine the mechanism of protein tyrosyl residue nitration by the EPO-H₂O₂-NO₂ system, we initially performed a variety of experiments utilizing N-acetyl-L-tyrosine, a low molecular weight surrogate for a protein-bound tyrosyl residue. In the absence of NO₂, incubation of N-acetyl-L-tyrosine with EPO and H₂O₂ resulted in formation of the N-acetyl dityrosine analog (Table IV). The addition of Br to the reaction mixtures resulted in production of the N-acetyl-L-tyrosine analogs of 3-bromotyrosine and 3,5-dibromotyrosine but no detectable production of the N-acetyl-L-tyrosine analog of the tyrosyl radical-addition product o,o'-dityrosine. In contrast, the addition of NO₂ to N-acetyl-L-tyrosine and the EPO-H₂O₂ system formed predominantly the N-acetyl-L-tyrosine analog of 3-nitrotyrosine and modest levels of the N-acetyl-L-tyrosine analog of o,o'-dityrosine (Table IV). The addition of both NO₂ and Br to N-acetyl-L-tyrosine and the EPO-H₂O₂ system formed all of the expected tyrosine oxidation products (N-acetyl-L-tyrosine analogs of 3-nitrotyrosine, o,o'-dityrosine, 3-bromotyrosine, and 3,5-dibromotyrosine). Thus, in every instance where tyrosine nitration occurred, concurrent formation of tyrosyl radical addition products was observed. In contrast, during tyrosine bromination (in the absence of NO₂), no formation of tyrosyl radical addition products (i.e. N-acetyl-L-tyrosine analog of o,o'-dityrosine) could be detected. These results are consistent with 3-nitrotyrosine production through an addition reaction between an intermediate tyrosyl radical and reactive nitrogen species such as ^·NO₂, the one electron oxidation product of NO₂.

View this table: [in this window] [in a new window]   Table IV Oxidation of N-acetyl tyrosine by eosinophil peroxidase-generated oxidants and by HOBr with or without NO2 N-Acetyl tyrosine (N-Ac-Y, 250 nmol) was mixed with the indicated additions in 0.5 ml of sodium phosphate buffer (20 mM, pH 7.0). Reactions were initiated by the addition of 250 nmol of oxidant (H2O2 or HOBr) and incubated at 37 °C for 1 h. The quantities of the N-acetyl derivatives of o,o'-dityrosine (N-Ac-di-Y), 3-nitrotyrosine (N-Ac-3-NO2-Y), 3-bromotyrosine (N-Ac-3-Br-Y), and 3,5-dibromotyrosine (N-Ac-3,5-diBr-Y) formed were then determined by reverse phase HPLC with UV absorbance (A280) detection as described under "Experimental Procedures." Product identity was confirmed by concomitant on-line ESI-MS analyses as described under "Experimental Procedures." The concentrations of the following reagents (when present) were as follows: eosinophil peroxidase (57 nM), NaBr (1 mM), NaNO2 (1 mM). Results shown are the mean ± S.D. of three independent experiments. The limit of detection for these analyses is <0.1 nmol for each analyte examined.

Co-incubation of HOCl and NO₂ has been reported to form a nitrating and chlorinating intermediate, presumably NO₂Cl (58). Moreover, we observed modest increases in the levels of free and protein-bound nitrotyrosine formed during incubation with the EPO-H₂O₂ system in the presence of NO₂ and Cl compared with NO₂ alone (Tables II and III). However, the mechanism for the increase does not appear to involve reaction of HOCl with NO₂, since comparable increases in tyrosine nitration were observed in the presence of Cl during incubations that contained excess methionine, a potent scavenger of HOCl and other halogenating intermediates (data not shown). To determine if EPO might indirectly promote protein nitration by forming a nitrating intermediate by oxidation of NO₂ with HOBr, we performed the following experiment. N-Acetyl-L-tyrosine was incubated with HOBr in the presence and absence of NO₂, and the extent of tyrosine nitration was determined. No detectable formation of the 3-nitrotyrosine analog was observed, even in the presence of 1 mM concentrations of both HOBr and NO₂ (Table IV). Similar results were observed in reactions performed under acidic (pH 4) conditions (data not shown). Collectively, these results suggest that the nitrating intermediate formed by EPO arises from direct oxidation of NO₂ and not by secondary oxidation of NO₂ by HOCl, HOBr, or some other halogenating agent.

In a final series of experiments, we sought to further explore the potential role of a tyrosyl radical intermediate in 3-nitrotyrosine formation by monitoring the extent of aromatic nitration using tyrosine analogs that do not form tyrosyl radicals. Because of its acidic character, the phenoxyl hydrogen of L-tyrosine is the preferred site of hydrogen atom abstraction from tyrosine; consequently, O-methyl-L-tyrosine is resistant to formation of tyrosyl radical (60). If 3-nitrotyrosine production by the EPO-H₂O₂-NO₂ system instead occurred by an electrophilic addition reaction (e.g. through a NO₂⁺ intermediate), use of the O-methyl-L-tyrosine analog should not significantly effect the yield of 3-nitrotyrosine formation. Incubation of the complete EPO-H₂O₂-NO₂ system with L-tyrosine readily formed 3-nitrotyrosine; in contrast, no nitrated products were formed from O-methyl-L-tyrosine at all concentrations of NO₂ examined, as determined by reverse phase HPLC with on-line UV and ESI/MS (Fig. 11). Collectively, these results strongly support the hypothesis that the EPO-H₂O₂-NO₂ system nitrates tyrosyl residues through a tyrosyl radical intermediate.

View larger version (12K): [in this window] [in a new window]   Fig. 11.   Examination of eosinophil peroxidase-mediated nitration of L-tyrosine versus O-methyl-L-tyrosine. Either L-tyrosine (100 µM) or O-methyl-L-tyrosine (100 µM) were incubated with eosinophil peroxidase (57 nM), H2O2 (100 µM), and the indicated concentrations of NO2 in sodium phosphate buffer (20 mM, pH 7.0) at 37 °C for 60 min. The content of 3-nitrotyrosine and O-methyl-3-nitrotyrosine formed was then quantified by reverse phase HPLC as described under "Experimental Procedures." Results represent the mean of triplicate determinations from a typical experiment performed in duplicate.

	DISCUSSION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

Eosinophils play an essential role in tissue surveillance and host defense mechanisms (1-3). These cells have evolved enzymatic mechanisms to inflict oxidative damage upon invading parasites, pathogens, and cancer cells. The reactive species they form, however, also have the potential to harm host tissues and contribute to inflammatory tissue injury. Microbicidal and cytotoxic oxidants generated by the EPO-H₂O₂ system are thought to participate in promoting many of these functions (2-8). However, despite the numerous links between EPO, oxidant production, and tissue injury in eosinophilic inflammatory disorders, structural identification of oxidation products formed by the action of EPO on target proteins is lacking.

The present studies identify aromatic nitration reactions as a new category of oxidation reactions mediated by EPO. EPO readily used NO₂, a major end product of ^·NO metabolism, as substrate to generate a reactive intermediate that nitrates free and protein-bound tyrosyl residues in high yield. Multiple independent analytical methods confirmed the structure of the major tyrosine oxidation product formed by the EPO-H₂O₂-NO₂ system as 3-nitrotyrosine: (i) studies employing LC/MS with electrospray ionization detection demonstrated that the retention time and mass spectrum of the oxidation product were identical to that of authentic 3-nitrotyrosine; (ii) multiple distinct derivatives of the major tyrosine oxidation product formed by the EPO-H₂O₂-NO₂ system demonstrated identical GC retention times and negative ion electron impact mass spectra to that of similarly derivatized authentic 3-nitrotyrosine and co-migrated with synthetically prepared 3-nitro-[¹³C₆]tyrosine (both in native form and following reduction of both to 3-aminotyrosine); (iii) the UV-visible absorbance spectra of the tyrosine oxidation product was identical to that of authentic 3-nitrotyrosine at acidic, neutral, and basic pH; (iv) the retention time and electrochemical properties (i.e. hydrodynamic voltamogram) of the EPO-generated product were identical to those of authentic 3-nitrotyrosine when analyzed by reverse phase HPLC with electrochemical (coulometric array) detection; and finally (v) antibodies raised against 3-nitrotyrosine specifically recognize protein oxidized by the EPO-H₂O₂-NO₂ system. Collectively, these results unambiguously identify 3-nitrotyrosine as a product formed by action of the EPO-H₂O₂-NO₂ system on free and protein-bound tyrosine residues.

The present results also provide the first evidence for tyrosyl radical production by EPO. EPO-dependent formation of this diffusible radical species is strongly supported by the structural identification of multiple EPO-generated phenolic addition products of tyrosyl radical (e.g. o,o'-dityrosine, isodityrosine, pulcherosine, and trityrosine). Moreover, the mechanism of aromatic nitration reactions mediated by the EPO-H₂O₂-NO₂ system is consistent with formation of a tyrosyl radical intermediate. Thus, tyrosyl radical-dependent oxidative damage of biological targets represents another potential pathway that EPO may contribute to inflammatory tissue injury.

One of the remarkable features of protein nitration by the EPO-H₂O₂-NO₂ system is the overall high yield of 3-nitrotyrosine formation. In the presence of plasma levels of halides and levels of NO₂ that approximate those found in inflammatory tissues and fluids, 3-nitrotyrosine was a preferred product formed with both free and protein-bound tyrosine residues as targets (e.g. Tables II and III). Moreover, tyrosine nitration was optimal in a physiological pH range (6.0-7.0) and accounted for between 10 and 20% of the H₂O₂ consumed during oxidation of proteins by the EPO-H₂O₂-NO₂ system at neutral pH. Finally, common nucleophilic scavengers such as primary amines, thiols, and thiol ethers only modestly attenuated nitrotyrosine formation; however, physiological levels of glutathione, ascorbate, and SCN are anticipated to have a major impact on the extent of nitrotyrosine formation by EPO. Taken together, these results suggest that EPO-dependent nitration of protein tyrosyl residues is likely to occur in vivo, particularly at sites where eosinophilic infiltration and ^·NO (and hence NO₂) production are enhanced and ascorbate, glutathione, and SCN levels are limiting. Indeed, recent immunohistochemical studies using antibodies raised against nitrotyrosine demonstrated intense staining over eosinophils and throughout eosinophil-rich tissues of individuals with severe asthma (22), a condition where eosinophils are implicated in promoting inflammatory tissue injury and enhanced oxidant stress and ^·NO production occur (2-6, 61-64). Although the authors interpreted the 3-nitrotyrosine immunostaining as evidence of peroxynitrous acid production (22), the current results suggest that an alternative source may be the EPO-H₂O₂-NO₂ system of eosinophils.

It is interesting to note that the extent of 3-nitrotyrosine formation by EPO is modulated by physiologically relevant levels of SCN. Thiocyanate ion is produced following the enzymatic hydrolysis of certain plant glycosides. Thus, eating certain vegetables (e.g. cabbage) results in a transient increase in the concentration of thiocyanate in blood and urine (57). Normal plasma levels of SCN are under 70 µM (57), but higher levels may be observed following excessive tobacco smoking or prolonged intravenous administration of sodium nitroprusside, a potent vasodilator used in critically ill patients (57, 65). Eosinophils perform their biological functions in a large variety of tissues and fluids whose concentration of halides, NO₂, and SCN may differ considerably from plasma. Indeed, our kinetic studies (Table I) suggest that at plasma concentrations of substrates (SCN, Br, NO₂, and Cl), EPO is far from saturated. Thus, the relative contribution of EPO to 3-nitrotyrosine formation in vivo may be tissue-specific, even at sites of inflammation characterized by eosinophil recruitment and activation.

The formation of reactive nitrogen species by direct peroxidase-dependent oxidation of NO₂, or through oxidation of NO₂ by oxidants such as HOCl, has recently been reported (25-28, 58, 59). Indeed, the ability of MPO secreted from activated neutrophils to catalyze aromatic nitration reactions has suggested that nitrotyrosine formation at sites of inflammation may arise from pathways independent of peroxynitrous acid generation (26). However, whether EPO could catalyze similar reactions given the unusual substrate preferences, distinct prosthetic heme group, and unique physical properties of this highly cationic protein was unclear. The results of the present studies demonstrate that aromatic nitration reactions are indeed a preferred activity of the enzyme. Moreover, the nitrating capacity of EPO was shown to exceed that of the related leukocyte-derived peroxidase, MPO, by at least 4-fold at physiological concentrations of halides and every concentration of NO₂ examined. Finally, it should be noted that O₂ and H₂O₂ production by activated eosinophils is severalfold greater than that of an equal number of comparably stimulated neutrophils (9, 10) and that the total content of EPO in eosinophils is reported to be 2-4-fold higher than that of MPO in neutrophils (e.g. 30-33 pmol of MPO per 1 × 10⁶ neutrophils versus 70-135 pmol of EPO per 1 × 10⁶ eosinophils) (16, 66, 67). Thus, EPO-dependent formation of reactive nitrogen intermediates is likely to occur in vivo, particularly during inflammatory disorders characterized by the presence of eosinophils.

Another interesting distinction between EPO- and MPO-dependent nitration reactions arises from their differing halide specificity. Although MPO generates chlorinating oxidants at physiological concentrations of halides (46, 68-70), and HOCl is reported to react with NO₂ to form a nitrating intermediate (58), we found no evidence for a parallel reaction between NO₂ and HOBr, the primary halogenating intermediate formed by EPO at plasma levels of halides. Moreover, although EPO-dependent nitration of tyrosine was optimal in the presence of both NO₂ and plasma levels of Cl (e.g. see Tables II and III), the addition of methionine, a potent scavenger of halogenating intermediates (46, 71, 72), failed to attenuate nitrotyrosine formation by EPO. The mechanism of the modest but reproducible increase in both free and protein-bound 3-nitrotyrosine formation observed in the presence versus absence of Cl is unclear. However, the inability of methionine to attenuate tyrosine nitration by EPO in the presence of either Br or Cl suggests that indirect oxidation of NO₂ by an EPO-generated hypohalous acid does not contribute significantly to tyrosine nitration.

One interesting question is the chemical nature of the reactive nitrogen species formed by EPO-catalyzed oxidation of NO₂. Peroxidases catalyze both one- and two-electron oxidation reactions, and both the one-electron (i.e. nitrogen dioxide) and two-electron (e.g. peroxynitrous acid) oxidation products of NO₂ have been suggested as potential intermediates formed by MPO (25, 28). Both intermediates can form nitrotyrosine, as well as abstract a phenoxyl hydrogen atom to form tyrosyl radical intermediates. Future studies aimed at elaborating the oxidant(s) formed and the mechanisms of free and protein-bound tyrosine nitration mediated by peroxidase-dependent oxidation of NO₂ are warranted.

Eosinophils are typically rare leukocytes with a limited circulating life span (t_1/2 ~ 2 h) before they take residence in tissues (1). However, they do play a prominent role in certain forms of inflammation (e.g. allergic), where their numbers in tissues can greatly exceed those of other leukocytes (1-6). Indeed, some of the most devastating inflammatory disorders in humans are associated with intense eosinophilic infiltration (1-6, 73, 74). Identifying the mechanisms and products of EPO-dependent oxidative damage is a critical step toward development of targeted interventions designed to interrupt oxidative tissue injury in eosinophilic inflammatory disorders.

	ACKNOWLEDGEMENTS

ESI/MS experiments were performed at the Cleveland Mass Spectrometry Core Facility. We thank Mary Katherine Fraser for assistance in the preparation of this manuscript.

	FOOTNOTES

^* This work was supported in part by a Scientist Development Grant from the American Heart Association and by National Institutes of Health Grants HL61878 and HL62526.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Cleveland Clinic Foundation, Lerner Research Institute, Dept. of Cell Biology, 9500 Euclid Ave., NC-10, Cleveland, OH 44195. Tel.: 216-445-9763; Fax: 216-444-9404; E-mail: hazens@ccf.org.

	ABBREVIATIONS

The abbreviations used are: EPO, eosinophil peroxidase; DTNB, 5,5'-dithiobis(2-nitrobenzoic acid); ESI, electrospray ionization; GC, gas chromatography; HOBr, hypobromous acid; HOCl, hypochlorous acid; HPLC, high performance liquid chromatography; H₂O₂, hydrogen peroxide; LC, liquid chromatography; MPO, myeloperoxidase; MS, mass spectrometry; NO, nitrogen monoxide; NO₂, nitrite; TNB, 5-thio-2-nitrobenzoic acid; BSA, bovine serum albumin.

	REFERENCES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES

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