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J Biol Chem, Vol. 274, Issue 36, 25953-25957, September 3, 1999

Homer 1b Regulates the Trafficking of Group I Metabotropic Glutamate Receptors^*

Katherine W. Roche^§, Jian Cheng Tu^¶, Ronald S. Petralia, Bo Xiao^¶, Robert J. Wenthold, and Paul F. Worley^¶

From the  Laboratory of Neurochemistry, NIDCD, National Institutes of Health, Bethesda, Maryland 20892 and the ^¶ Department of Neuroscience, The Johns Hopkins University, Baltimore, Maryland 21205

	ABSTRACT

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS AND DISCUSSION REFERENCES

The molecular basis for glutamate receptor trafficking to the plasma membrane is not understood. In the present study, we demonstrate that Homer 1b (H1b), a constitutively expressed splice form of the immediate early gene product Homer (now termed Homer 1a) regulates the trafficking and surface expression of group I metabotropic glutamate receptors. H1b inhibits surface expression of the metabotropic glutamate receptor mGluR5 in heterologous cells, causing mGluR5 to be retained in the endoplasmic reticulum (ER). In contrast, mGluR5 alone or mGluR5 coexpressed with Homer 1a successfully travels through the secretory pathway to the plasma membrane. In addition, point mutations that disrupt mGluR5 binding to H1b eliminate ER retention of mGluR5, demonstrating that H1b affects metabotropic receptor localization via a direct protein-protein interaction. Electron microscopic analysis reveals that the group I metabotropic receptor mGluR1 is significantly enriched in the ER of Purkinje cells, suggesting that a similar mechanism may exist in vivo. Because H1b is found in dendritic spines of neurons, local retention of metabotropic receptors within dendritic ER provides a potential mechanism for regulating synapse-specific expression of group I metabotropic glutamate receptors.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS AND DISCUSSION REFERENCES

The responsiveness of a neuron to neurotransmitter released from a presynaptic cell is determined by the type and amount of receptor expressed on the postsynaptic membrane. Glutamate is the most prevalent excitatory neurotransmitter in the mammalian central nervous system, and it has multiple receptor subtypes that have unique distributions throughout the brain. The complex and distinct synaptic localization of different glutamate receptors in central nervous system neurons (1, 2) requires highly selective intracellular targeting mechanisms. The distributions of individual receptors within neurons are not uniform, including differences related to specific synaptic populations (3-5) as well as pre- and postsynaptic locations (1, 2, 6, 7). Since the initial observation that PSD-95 interacts with N-methyl-D-aspartate receptors (8), it has been proposed that PDZ domain-containing proteins such as PSD-95 play a role in clustering and/or anchoring of glutamate receptors at synapses (9-12). The synaptic expression of glutamate receptors may be controlled exclusively by regulating their interactions with these anchoring proteins, or additional mechanisms may exist which regulate the intracellular targeting and availability of receptors for synaptic insertion.

Homer, now termed Homer 1a, was originally identified as a brain immediate early gene product based on its rapid and transient induction in hippocampal neurons following synaptic activation (13). Recently, we have reported a novel family of Homer-related proteins that possess a putative N-terminal EVH1 domain that confers the capacity to bind the C terminus of group I metabotropic receptors (14). Newly identified family members include two splice variants of the Homer 1 gene, termed Homer 1b and Homer 1c, as well as two different Homer genes, termed Homer 2 and Homer 3. Unlike the original immediate early gene form, all subsequently identified Homer family members are constitutively expressed in brain and encode an additional C-terminal coiled-coil motif. This motif mediates protein-protein interactions, including self-multimerization (14).

In the present study, we demonstrate that H1b,¹ unlike H1a, inhibits surface expression of mGluR5 when the two proteins are coexpressed in heterologous cells. Using immunofluorescent and biochemical techniques, we show that H1b causes mGluR5 to be retained within the ER. The effect of H1b on the trafficking of mGluR5 requires a direct interaction between mGluR5 and H1b and is not observed when mGluR5 is coexpressed with the immediate early gene form H1a, which binds metabotropic receptor but lacks the C-terminal coiled-coil domain. Although these experiments were performed in heterologous cells, we also observe enrichment of the group I metabotropic receptor mGluR1 in the ER of Purkinje cells. This suggests H1b-regulated ER retention may occur in vivo, possibly revealing a novel mechanism for synapse-specific receptor expression.

	EXPERIMENTAL PROCEDURES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS AND DISCUSSION REFERENCES

cDNA Constructs-- mGluR1, mGluR2, and mGluR5 cDNAs were generously provided by S. Nakanishi, and each was subcloned into the mammalian expression vector pRK5. H1a and H1b were also subcloned into pRK5 and included an N-terminal Myc epitope.

Cell Culture and Transfections-- HeLa cells were grown on 10-cm dishes for biochemical analyses and on glass coverslips in 6-well tissue culture dishes for immunofluorescence microscopy. HeLa cells were transfected with mGluR5, mGluR1, or mGluR2 cDNAs with empty vector, H1a, or H1b cDNAs (10 µg total/10-cm dish or 5 µg total/well of 6-well dish) using the calcium phosphate coprecipitation method (15). Transfected cells were analyzed 36 h after transfection.

Antibodies-- Antibodies against mGluR5 (13), H1b (14), mGluR2/3 (7), mGluR1 (16), and Myc (17) (9E10) have been characterized previously. The BiP mouse monoclonal antibodies were obtained from StressGen Biotechnologies Corp. The N-mGluR5 antibodies were generated by immunizing rabbits with the synthetic peptide RLEGFAQENSKYNKTC (mGluR5 amino acids 365-380).

Surface Labeling-- Transfected HeLa cells grown on coverslips were washed in PBS, incubated with primary antibodies (6 µg/ml affinity-purified N-mGluR5 antibodies in PBS containing 3% NGS) on ice for 1.5 h, washed in PBS, and fixed in 4% paraformaldehyde in PBS for 20 min. The coverslips were then washed in PBS, incubated in secondary antibodies (Cy3 anti-rabbit diluted 1:500 in PBS containing 1% bovine serum albumin and 1% saponin) at room temperature for 30 min, and then washed in PBS and mounted onto slides using Vectashield mounting media.

Immunocytochemistry-- Transfected HeLa cells grown on coverslips were washed in PBS, fixed in 4% paraformaldehyde in PBS for 20 min, washed in PBS, and permeabilized in 0.25% Triton X-100 in PBS for 5 min. The coverslips were washed in PBS and incubated with primary antibodies (mGluR5 antisera, 1:1000; Myc, 1:1000; mGluR1 antibodies, 1 µg/ml; mGluR2/3 affinity-purified antibodies, 1 µg/ml; or BiP ascites, 1:500 in PBS containing 3% NGS) for 1-2 h at room temperature, washed in PBS, and incubated with secondary antibodies (Cy3 anti-rabbit or Cy3 anti-mouse diluted 1:500 in PBS containing 3% NGS for single label or fluorescein isothiocyanate anti-mouse and rhodamine anti-rabbit diluted 1:500 in PBS containing 3% NGS for double label) for 30 min at room temperature, washed in PBS, and then mounted with Vectashield mounting media.

Glycosidase Treatment-- Transfected HeLa cells grown on 10-cm dishes were washed in TBS (2 mM EDTA, 0.1 mM AEBSF 4-(2-aminoethyl)benzenesulfonyl fluoride), 1 µg/ml leupeptin, 5 mM iodoacetamide), collected in lysis buffer without detergent, and sonicated, and total membranes were collected. The membranes were resuspended in 50 µl of lysis buffer with 1% SDS and heated at 100 °C for 5 min. Then 5 volumes of cold 1% octyl glucoside was added, and the samples were divided three ways. The samples were incubated alone, with endoglycosidase H (Endo H), or with N-glycosidase F (6 units/ml) for 4-5 h at 37 °C, then frozen until further analysis. Samples were thawed, sample buffer was added, and the samples were incubated at 100 °C for 5 min before loading on the gel. The proteins were resolved by SDS-polyacrylamide gel electrophoresis on 4-20% (Fig. 4A) or 4-12% (Fig. 3) gradient gels. The proteins were transferred to polyvinylidene difluoride membranes, immunoblotted with mGluR5 antisera diluted 1:1000, and visualized with enhanced chemiluminescence.

Generation of Point Mutations-- Point mutations within the Homer binding site of mGluR5 were generated using the QuikChange method (Stratagene) and are described in Tu et al. (22).

EM Analysis-- The postembedding immunogold method has been described previously (18-20) and is modified from the method of Matsubara et al. (21). Male Sprague-Dawley rats were perfused with 4% paraformaldehyde plus 0.5% glutaraldehyde in 0.1 M phosphate buffer. Parasagittal sections of the rostral cerebellum (folia III-V) were cryoprotected in 30% glycerol, frozen in liquid propane in a Leica EM CPC, immersed in 1.5% uranyl acetate in methanol at 90 °C in a Leica AFS freeze-substitution instrument, infiltrated with Lowicryl HM 20 resin at 45 °C, and polymerized with UV light. Thin sections were incubated in 0.1% sodium borohydride plus 50 mM glycine in Tris-buffered saline/0.1% Triton X-100 (TBST), followed by 10% NGS in TBST, primary antibody in 1% NGS/TBST, 10 nm immunogold (Amersham Pharmacia Biotech) in 1% NGS/TBST plus 0.5% polyethylene glycol, and finally stained with uranyl acetate and lead citrate. mGluR1 antibody was used at a dilution of 1:25.

	RESULTS AND DISCUSSION

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS AND DISCUSSION REFERENCES

To determine whether different Homer family members have distinct functional roles in metabotropic receptor localization, we first investigated the effect of the Homer 1 gene products, H1a and H1b, on cell surface expression of mGluR5. We chose to coexpress mGluR5 with H1a or with H1b in heterologous cells, to allow independent characterization of the different Homer splice variants. To specifically label surface-expressed receptors, we used an antibody (N-mGluR5) raised against an extracellular epitope of mGluR5 to stain live cells for microscopic analysis. mGluR5 was expressed on the surface of HeLa cells when expressed alone or when coexpressed with H1a. Surprisingly, mGluR5 did not reach the cell surface when coexpressed with the alternatively spliced long form of Homer 1, H1b (Fig. 1). Importantly, both immunofluorescent analysis of permeabilized cells and Western blot analysis of cell extracts revealed that total mGluR5 expression was not affected by cotransfection with H1b (Figs. 2A and 4).

View larger version (43K): [in this window] [in a new window]   Fig. 1.   H1b inhibits surface expression of mGluR5. HeLa cells were transfected with mGluR5 and empty vector (top panel), mGluR5 and H1a (middle panel), or mGluR5 and H1b (bottom panel). In each condition, the cells were labeled live with N-mGluR5 antibodies on ice and fixed, and surface-expressed mGluR5 was visualized with Cy3-conjugated secondary antibodies.

View larger version (42K): [in this window] [in a new window]   Fig. 2.   mGluR5 is retained in the ER when coexpressed with H1b. A, distribution of mGluR5 expressed alone, with H1a, or with H1b. HeLa cells were transfected with mGluR5 and empty vector (top panel), mGluR5 and H1a (middle panel), or mGluR5 and H1b (bottom panel). In each condition, the cells were fixed, permeabilized, and labeled with mGluR5 antibodies. The cells were then visualized with Cy3-conjugated secondary antibodies. B, colocalization of the ER marker BiP with mGluR5 when mGluR5 is coexpressed with H1b. HeLa cells were cotransfected with mGluR5 and H1b and double-labeled with BiP (left panel) and mGluR5 antibodies (right panel).

View larger version (44K): [in this window] [in a new window]   Fig. 3.   H1b colocalizes with mGluR5 in the ER. HeLa cells were cotransfected with mGluR5 and H1b. The cells were then fixed, permeabilized, and double-labeled with mGluR5 (left panel) and Myc (right panel) antibodies. The cells were visualized with rhodamine-conjugated anti-rabbit and fluorescein isothiocyanate-conjugated anti-mouse secondary antibodies.

In an attempt to understand the mechanism by which H1b regulates mGluR5 surface expression, we characterized the intracellular distribution of mGluR5 using immunofluorescence of permeabilized cells. mGluR5 expressed alone or with H1a was distributed diffusely throughout the cell. In contrast, mGluR5 expressed with H1b had a dramatically different subcellular localization. mGluR5 was present in perinuclear organelles as well as extensive reticular staining present throughout the cell that strongly resembled that of the ER (Fig. 2A). To confirm that the effect of H1b was not restricted to mGluR5, we expressed H1b with another group I metabotropic glutamate receptor, mGluR1, and analyzed the intracellular distribution of the glutamate receptor. Like mGluR5, mGluR1 appeared to be localized to ER when coexpressed with H1b (data not shown).

To conclusively identify the intracellular compartment containing mGluR5, we double-labeled mGluR5/H1b cotransfected cells with antibodies recognizing mGluR5 and the ER-resident protein BiP. Staining with BiP antibodies revealed the extensive ER present in both transfected and untransfected cells. The mGluR5 antibodies only recognized transfected cells and revealed colocalization with the BiP-positive reticulum and the perinuclear organelles, confirming that mGluR5 was present within the ER and ER-derived structures (Fig. 2B). It should be noted that the perinuclear organelles were not present within nontransfected cells and therefore appear to be ER-derived structures unique to cells overexpressing mGluR5 and H1b. These observations suggest that H1b, but not H1a, causes mGluR5 to be retained in the ER.

We also performed double-labeling immunofluorescent microscopy of mGluR5 and H1b when these two proteins were coexpressed in HeLa cells. mGluR5 and H1b displayed considerable colocalization (Fig. 3) with bright immunoreactivity in the ER and perinuclear organelles. Although complete colocalization was common, we also often observed colocalization of the two proteins with excess plasma membrane localization of H1b.

As an additional assay for ER retention, we examined the status of the carbohydrates present on mGluR5 in cells expressing H1a or H1b. If H1b caused mGluR5 to be retained within the ER, then mGluR5 should contain immature, high mannose carbohydrates that are sensitive to digestion with the enzyme Endo H. Alternatively, if mGluR5 had successfully traveled through the ER and cis-Golgi, it would possess mature, complex carbohydrates that would be Endo H-resistant. Mature carbohydrates would be anticipated if mGluR5 was on the cell surface or if it was sequestered in a post-Golgi intracellular compartment such as endosomes. We prepared membranes from mock transfected cells, cells expressing mGluR5 alone, or cells coexpressing mGluR5 and H1a or H1b. These membranes were treated with no enzyme, Endo H, or N-glycosidase F, an enzyme that removes all Asn-linked carbohydrates. Immunoblotting with mGluR5 antibodies revealed that mGluR5 is Endo H-resistant when expressed alone or with H1a. However, when expressed with H1b, mGluR5 is Endo H-sensitive (Fig. 4), consistent with the hypothesis that expression of H1b leads to the retention of group I metabotropic glutamate receptors in the ER.

View larger version (13K): [in this window] [in a new window]   Fig. 4.   mGluR5 is Endo H-sensitive when coexpressed with H1b. HeLa cells were transfected with vector alone, mGluR5 with empty vector, mGluR5 with H1a, or mGluR5 with H1b. Membranes were collected and incubated alone, with Endo H or with N-glycosidase F, and resolved by SDS-polyacrylamide gel electrophoresis. Immunoblots were probed with mGluR5 antibodies.

We were surprised that coexpression of mGluR5 with H1b caused complete ER retention of mGluR5 as detected by surface staining (Fig. 1) and carbohydrate analysis (Fig. 4). To determine whether H1b was present at saturating concentrations when coexpressed with mGluR5, we varied the amount of H1b expressed with mGluR5 to determine whether there existed a dose-response effect of H1b on mGluR5 trafficking to the cell surface (Fig. 5). We transfected cells with constant amounts of mGluR5 (5 µg) and increasing amounts of H1b (0, 0.5, 1.5, and 5 µg) to evaluate the effect H1b had on mGluR5 localization. We found that low levels of H1b expression did not result in the ER retention of mGluR5, whereas higher concentrations did result in ER retention. All other experiments we conducted used equal amounts of mGluR5 and H1b cDNA and therefore reflect the effect H1b has on mGluR5 under saturating conditions.

View larger version (29K): [in this window] [in a new window]   Fig. 5.   ER retention of mGluR5 is dependent on H1b concentration. HeLa cells were transfected with 5 µg of mGluR5 cDNA and varying amounts of H1b cDNA (0, 0.5, 1.5, or 5 µg). The total amount of DNA used per transfection was held constant by the addition of empty vector. A, membranes were collected and incubated alone, with Endo H, or with N-glycosidase F. The samples were resolved by SDS-polyacrylamide gel electrophoresis, and immunoblots were probed with mGluR5 antibodies. B, total homogenates were resolved by SDS-polyacrylamide gel electrophoresis, and immunoblots were probed with Myc antibodies.

We next wanted to confirm that overexpression of H1b did not cause the nonspecific retention of proteins in the ER. The subcellular localization of the group II metabotropic glutamate receptor mGluR2 was the same whether expressed alone or with H1b (data not shown). In addition, we used a series of mGluR5 constructs containing point mutations within the Homer binding site and found that mutations that disrupt mGluR5/Homer interactions in vitro also prevent ER retention of mGluR5 coexpressed with H1b (Fig. 6). mGluR5 P1125L, which does not bind to Homer in vitro (22), was not retained in the ER when coexpressed with H1b. In contrast, mGluR5 S1126F, which does bind Homer in vitro, was ER-retained when coexpressed with H1b (Fig. 6A). Other point mutations in adjacent residues were analyzed, and the results were consistent with in vitro binding studies (summarized in Fig. 6B) (22), demonstrating that mGluR5 is retained within the ER by H1b only when its Homer binding site is intact.

View larger version (24K): [in this window] [in a new window]   Fig. 6.   H1b binding is required for ER retention of mGluR5. A, mGluR5 P1125L coexpressed with H1b is Endo H-resistant, but mGluR5 S1126F coexpressed with H1b is Endo H-sensitive. HeLa cells were transfected with mGluR5 wild type and empty vector, mGluR5 wild type and H1b, mGluR5 P1125L and H1b, or mGluR5 S1126F and H1b. Membranes were collected and incubated alone, with Endo H, or with N-glycosidase F, and immunoblots were probed with mGluR5 antibodies. B, summary of point mutations within the Homer binding site of mGluR5 and their importance for Homer-mGluR5 binding. Residues that are critical for binding in vitro (22) are also necessary for H1b-mediated ER retention of mGluR5. The Homer binding site of mGluR5 is shown with residues critical for binding indicated with bold type.

Although our results clearly demonstrate that H1b retains group I metabotropic receptors in the ER of heterologous cells, its role in neurons may be more complicated. Therefore, we used immunoelectron microscopy to determine the intracellular distribution of mGluR1, the group I metabotropic receptor expressed within Purkinje cells, which express high levels of H1b (14). In agreement with previous reports, mGluR1 is expressed at perisynaptic sites (20, 23) (Fig. 7, inset). Interestingly, we also find many examples in the cell body and dendrite of Purkinje cells where mGluR1 is concentrated in the ER (Fig. 7), indicating that a major pool of metabotropic glutamate receptors is retained in the ER. The retention in neurons is most apparent in ER of the cell body, although mGluR1 is also found associated with ER of the dendrite. A similar distribution for other metabotropic receptors or the ionotropic glutamate receptors has not been reported, although most receptors have a significant pool associated with a yet undefined membrane population. Thus it appears that group I metabotropic glutamate receptors, at least in some cell types, are enriched in the ER of neurons.

View larger version (128K): [in this window] [in a new window]   Fig. 7.   mGluR1 immunolabeling is concentrated in the endoplasmic reticulum in Purkinje cell bodies and in perisynaptic areas of parallel fiber synapses (inset). m, mitochondrion; n, nucleus; p, presynaptic terminal. Line scale is 0.2 µm.

In the present study, we investigated the role of H1b in the trafficking and organization of group I metabotropic receptors. Our results show that H1b inhibits the surface expression of mGluR5 when the two proteins are coexpressed in heterologous cells. Immunofluorescent microscopy and carbohydrate analysis of mGluR5 definitively reveal that the impaired surface expression is due to retention of mGluR5 in the ER. We also demonstrate that this ER retention requires a direct interaction between H1b and mGluR5. It is important to note that the ER retention of mGluR5 is specific for H1b because H1a has no effect on mGluR5 localization even though it possesses the same mGluR5 binding site. This finding implicates the C-terminal coiled-coil domain of H1b in ER retention. The coiled-coil domain mediates homomultimerization of H1b and produces a multivalent complex that could cross-link mGluRs to other proteins such as the IP3 receptor (22), which is located in the ER. Although other receptor-binding proteins have been shown to alter receptor distributions in heterologous cells (24-29), to our knowledge, this is the first demonstration of the ER retention of an integral membrane protein upon coexpression with a cytosolic binding protein. This novel finding supports the idea that proteins of the Homer family are involved in the transport and localization of group I metabotropic receptors in neurons.

Activity-regulated mechanisms have been implicated in the surface expression of mGluRs previously (30). Using synaptosomes prepared from the visual cortex, it was found that functional group I metabotropic glutamate receptors were developmentally regulated in normal animals but not in dark-reared animals. The activity-dependent increase in the expression of functional phosphatidylinositol-linked metabotropic glutamate receptors almost precisely parallels the expression of H1a observed in light-exposed versus dark-reared animals (13). These data are consistent with a model in which activity-regulated increases in H1a expression disrupt binding of H1b to mGluRs, leading to increased trafficking of metabotropic receptors to the plasma membrane and additional functional surface receptors.

In addition to the regulation of receptor transport, H1b could also form a functional link between the perisynaptic mGluRs and inositol 1,4,5-trisphosphate receptors in the dendritic ER (22). Recent findings demonstrate that H1b binds to inositol 1,4,5-trisphosphate receptors. Furthermore, it was demonstrated that H1a injected into Purkinje cells causes a reduction in calcium released from intracellular stores, consistent with the long Homer isoforms coupling metabotropic receptors to inositol 1,4,5-trisphosphate receptors (22). This is a provocative idea because the ER is the functional target of phosphatidylinositol-linked receptors such as group I metabotropic glutamate receptors. In both cases, H1a, the immediate early gene isoform of Homer, is anticipated to act locally to compete with the actions of the constitutively expressed Homer proteins, thereby regulating the function of group I metabotropic receptors. In support of this proposal, it was recently demonstrated that overexpression of H1a in transgenic mice disrupts mGluR5 binding to H1b (14), providing compelling evidence that H1a competes with longer isoforms for receptor binding in vivo. We believe competitive regulation of ER retention close to the postsynaptic membrane could therefore provide a sensitive mechanism for the regulation of synapse-specific surface expression of glutamate receptors.

	ACKNOWLEDGEMENT

We thank Dr. Y.-X. Wang for assistance in the immunogold immunocytochemistry.

	FOOTNOTES

^* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^§ To whom correspondence should be addressed: Laboratory of Neurochemistry, NIDCD, Bldg. 36, Rm. 5D08, National Institutes of Health, Bethesda, MD 20892. Tel: 301-496-3800; Fax: 301-480-3242; E-mail: rochek@nidcd.nih.gov.

	ABBREVIATIONS

The abbreviations used are: H1, Homer 1 protein; NGS, normal goat serum; PBS, phosphate-buffered saline; Endo H, endoglycosidase H; ER, endoplasmic reticulum.

	REFERENCES

TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS AND DISCUSSION REFERENCES

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				N. Breysse, C. Baunez, W. Spooren, F. Gasparini, and M. Amalric Chronic But Not Acute Treatment with a Metabotropic Glutamate 5 Receptor Antagonist Reverses the Akinetic Deficits in a Rat Model of Parkinsonism J. Neurosci., July 1, 2002; 22(13): 5669 - 5678. [Abstract] [Full Text] [PDF]

				A. Serge, L. Fourgeaud, A. Hemar, and D. Choquet Receptor Activation and Homer Differentially Control the Lateral Mobility of Metabotropic Glutamate Receptor 5 in the Neuronal Membrane J. Neurosci., May 15, 2002; 22(10): 3910 - 3920. [Abstract] [Full Text] [PDF]

				A. Oksche, G. Leder, S. Valet, M. Platzer, K. Hasse, S. Geist, G. Krause, A. Rosenthal, and W. Rosenthal Variant Amino Acids in the Extracellular Loops of Murine and Human Vasopressin V2 Receptors Account for Differences in Cell Surface Expression and Ligand Affinity Mol. Endocrinol., April 1, 2002; 16(4): 799 - 813. [Abstract] [Full Text] [PDF]

				A. Francesconi and R. M. Duvoisin Alternative Splicing Unmasks Dendritic and Axonal Targeting Signals in Metabotropic Glutamate Receptor 1 J. Neurosci., March 15, 2002; 22(6): 2196 - 2205. [Abstract] [Full Text] [PDF]

				J. J. Miret, L. Rakhilina, L. Silverman, and B. Oehlen Functional Expression of Heteromeric Calcitonin Gene-related Peptide and Adrenomedullin Receptors in Yeast J. Biol. Chem., February 22, 2002; 277(9): 6881 - 6887. [Abstract] [Full Text] [PDF]

				T. T. Diagana, U. Thomas, S. N. Prokopenko, B. Xiao, P. F. Worley, and J. B. Thomas Mutation of Drosophila homer Disrupts Control of Locomotor Activity and Behavioral Plasticity J. Neurosci., January 15, 2002; 22(2): 428 - 436. [Abstract] [Full Text] [PDF]